De-Zhong Zhang^*, Bing-He Chen^*, Lan-Fang Zhang^†, Ming-Kun Cheng^‡, Xiang-Jie Fang^*, Xin-Jun Wu^*

Oncology Research, Vol.25, No.9, pp. 1453-1462, 2017, DOI:10.3727/096504017X14886494526344

Abstract Gastric cancer (GC) is the most common epithelial malignancy worldwide. Basic transcription factor 3 (BTF3) plays a crucial role in the regulation of various biological processes. We designed experiments to investigate the molecular mechanism underlying the role of BTF3 in GC cell proliferation and metastasis. We confirmed that BTF3 expression was decreased in GC tissues and several GC cell lines. Lentivirus-mediated downregulation of BTF3 reduced cell proliferation, induced S and G₂/M cell cycle arrest, and increased apoptosis. Knockdown of BTF3 significantly reduced the expression of Forkhead box M1 (FOXM1). Upregulation of FOXM1 significantly inhibited the decrease… More >

Enrico Mini^*, Ida Landini^*, Laura Lucarini^†, Andrea Lapucci^*, Cristina Napoli^‡, Gabriele Perrone^*, Renato Tassi^*, Emanuela Masini^†, Flavio Moroni^†, Stefania Nobili^‡

Oncology Research, Vol.25, No.9, pp. 1441-1451, 2017, DOI:10.3727/096504017X14926854178616

Abstract The poly(ADP-ribose) polymerase (PARP) enzymes play a key role in the regulation of cellular processes (e.g., DNA damage repair, genomic stability). It has been shown that PARP inhibitors (PARPIs) are selectively cytotoxic against cells having dysfunctions in genes involved in DNA repair mechanisms (synthetic lethality). Drug-induced PARP inhibition potentiates the activity of anticancer drugs such as 5-fluorouracil in enhancing DNA damage, whose repair involves PARP-1 activity. The aim of this study was to evaluate the inhibitory effects of a novel PARPI, HYDAMTIQ, on growth in human tumor cell lines characterized by different features with regard… More >

Huimin Wang^*, Hexin Chen^†, Hang Zhou^*, Wenfa Yu^*, Zhenmin Lu^*

Oncology Research, Vol.25, No.9, pp. 1431-1440, 2017, DOI:10.3727/096504017X14835311718295

Abstract Nasopharyngeal carcinoma (NPC) is a common malignancy of the head and neck that arises from the nasopharynx epithelium and is highly invasive. Cyclin-dependent kinase inhibitor 3 (CDKN3) belongs to the dualspecificity protein phosphatase family, which plays a key role in regulating cell division. Abnormal expression of CDKN3 has been found in numerous types of cancer. In the current study, we explored the possible role of CDKN3 in cell proliferation, ability to invade, and radiosensitivity in NPC cells. We reported that CDKN3 was upregulated and p27 was downregulated in NPC tissues and is associated with a… More >

Shi Ying^*, Huang Jianjun^†, Yi Xue^*, Yu Shuwei^*, Zhang Liyuan^*, Wang Jie^*, Cheng Lixian^*

Oncology Research, Vol.25, No.9, pp. 1421-1430, 2017, DOI:10.3727/096504016X14826089198805

Abstract MicroRNAs are a class of small noncoding RNAs that function as critical gene regulators through targeting mRNAs for translational repression or degradation. In this study, we showed that the miR-133b expression level was decreased while the Sirt1 mRNA expression level was increased in osteosarcoma tissue and cell lines. A low expression of miR-133b was significantly associated with tumor size, distant metastasis, and advanced clinical stage. In addition, osteosarcoma patients with a low miR-133b expression showed a worse prognosis when compared to those with a high level of miR-133b expression. Thus, we identified Sirt1 as a More >

Rui Jiang^*, Chao Zhang^†, Guangyao Liu^*, Rui Gu^*, Han Wu^*

Oncology Research, Vol.25, No.8, pp. 1409-1419, 2017, DOI:10.3727/096504017X14882829077237

Abstract Osteosarcoma is the most common primary bone malignancy manifested predominantly in children and young adults. Studies indicate that miR-107 is involved in the pathogenesis of osteosarcoma and that tropomyosin 1 (TPM1) acts as a tumor suppressor in many types of cancer. In this study, we analyzed the effect of miR-107 on human osteosarcoma cells and investigated the mechanism in which TPM1 is involved. miR-107 expression in human osteosarcoma tissues and cells was analyzed in quantitative real-time PCR (qRT-PCR). Human osteosarcoma (U2OS) cells were transfected with miR-107 mimic, inhibitor, or scramble controls to evaluate the effect… More >

Zhuang Kun^*†, Guo Hanqing^*, Tang Hailing^*, Yan Yuan^*, Zhang Jun^†, Zhang Lingxia^*, Han Kun^*, Zhang Xin^*

Oncology Research, Vol.25, No.8, pp. 1399-1407, 2017, DOI:10.3727/096504016X14823648620870

Abstract Gastric cancer (GC) is one of the most frequent epithelial malignancies worldwide. The gastrointestinal (GI) peptide gastrin is an important regulator of the secretion and release of gastric acid from stomach parietal cells, and it also plays a vital role in the development and progression of GC. The aim of the current study was to investigate the role and underlying mechanism of gastrin and autophagy in regulating GC tumorigenesis. Gastrin-17 amide (G-17) was applied in the GC cell lines SGC7901 and MGC-803. The results showed that G-17 maintained the high viability of SGC7901 and MGC-803.… More >

Ya-Li Gao^*1, Zi-Shen Zhao^†1, Ming-Yun Zhang^*, Li-Jie Han^*, Yu-Jin Dong^‡, Bo Xu^‡

Oncology Research, Vol.25, No.8, pp. 1391-1398, 2017, DOI:10.3727/096504017X14881559833562

Abstract Emerging evidence suggests that the long noncoding RNA (lncRNA) plasmacytoma variant translocation 1 (PVT1) gene is involved in the pathogenesis of cervical cancer. However, the potential mechanism is rarely reported. Our study found that PVT1 was upregulated in cervical cancer tissue and cell lines. After transfecting PVT1 siRNA, the proliferation, migration, and invasion of cervical cancer cells were markedly decreased. miRNA expression profiles demonstrate that miR-424 was markedly downregulated in cervical cancer tissue. Bioinformatics analysis revealed that miR-424 was potentially targeted by PVT1, which was confirmed by dual-luciferase reporter assay. Pearson’s correlation analysis showed that More >

Xiaoyu Wu^*1, Jin Zhou^†1, Zhenfeng Wu^*, Che Chen^*, Jiayun Liu^*, Guannan Wu^*, Jing Zhai^*, Fukun Liu^*, Gang Li^‡

Oncology Research, Vol.25, No.8, pp. 1383-1390, 2017, DOI:10.3727/096504017X14879366402279

Abstract miR-101-3p has been identified as a tumor suppressor in several cancers, but its exact role in gastric adenocarcinoma is still largely unknown. In this study, we found that, compared with the RGM-1 human normal gastric epithelial cells, miR-101-3p was significantly downregulated in all six human gastric adenocarcinoma cell lines, including BGC-823, MNK-45, MGC-803, SGC-7901, AGS, and HGC-27. Overexpression of miR- 101-3p suppressed both the proliferation and invasion of AGS gastric adenocarcinoma cells, and knockdown of miR-101-3p displayed the opposite effect. In addition, miR-101-3p could directly target and suppress the expression of the serum response factor More >

Qingchun Li^*, Yuan Tian^†, Guangrui Hu^†, Yun Liang^†, Wei Bai^†, Hongjun Li^†

Oncology Research, Vol.25, No.8, pp. 1373-1382, 2017, DOI:10.3727/096504017X14878509668646

Abstract Long noncoding RNA (lncRNA) antisense noncoding RNA in the INK4 locus (ANRIL) is involved in several human cancers. However, the role of ANRIL in renal cell carcinoma (RCC) remains unclear. This study aimed to explore whether, and how, ANRIL affects the progression of RCC. First, the expression of ANRIL in clinical tumor tissues and four kinds of RCC cell lines was evaluated. After transfection, cell viability, colony number, apoptosis, migration, and invasion were assessed. The expression of proteins related to apoptosis, epithelial-tomesenchymal transition (EMT), and the β-catenin signaling pathway was then assessed. In addition, the… More >

Shuke Ge^*, Dan Wang^†, Qinglong Kong^‡, Wei Gao^*, Jiayi Sun^*

Oncology Research, Vol.25, No.8, pp. 1363-1371, 2017, DOI:10.3727/096504017X14878536973557

Abstract miR-152, as a tumor suppressor, has been reported to be downregulated in a number of cancer cell lines and tumor tissues, including breast cancer. This study aimed to investigate the role of miR-152 in human breast cancer and its underlying mechanisms. Human breast cancer cell line HCC1806 was transfected with hsa-miR- 152-3p mimic, inhibitor, or scrambled negative controls. The efficiency of miR-152-3p transfection was evaluated by quantitative real-time PCR, and the effects on cell viability and apoptosis as well as on the PI3K/AKT signaling pathway were investigated by MTT assay, flow cytometry, and Western blot… More >