Junkai Chang^*1, Weibo Xu^*1, Guangchao Liu^†, Xinyi Du^*, Xiaodong Li^*

Oncology Research, Vol.25, No.2, pp. 241-248, 2017, DOI:10.3727/096504016X14742891049118

Abstract Rab23, a novel member of the Rab GTPase family, was found to be implicated in the progression of some human cancers. However, what role Rab23 plays in prostate cancer (PCa) remains to be illustrated. In the present study, we investigated the expression pattern and roles of Rab23 in PCa. The study results showed that Rab23 was upregulated in PCa tissues and cell lines. Moreover, downregulation of Rab23 remarkably suppressed the proliferation, migration, and invasion of PCa cells. In addition, downregulation of Rab23 significantly downregulated the protein expression levels of Shh and Gli1. Furthermore, we found More >

Min Li^*†, Mao Zhang^*, Zhi-lang Zhang^*, Ning Liu^*, Xiao-yu Han^*, Qin-cheng Liu^*, Wei-jun Deng^†, Cai-xian Liao^*

Oncology Research, Vol.25, No.2, pp. 233-239, 2017, DOI:10.3727/096504016X14742891049073

Abstract Hepatocellular carcinoma (HCC) is highly resistant to traditional chemotherapeutic approaches, which causes difficulty in the development of effective drugs for the treatment of HCC. Berberine, a major ingredient of Rhizoma coptidis, is a natural alkaloid used in traditional Chinese medicine. Berberine exhibits potent antitumor activity against HCC due to its high efficiency and low toxicity. In the present study, we found that berberine sensitized HepG cells to NF-kB-mediated apoptosis. Berberine exhibited a significant antiproliferation effect on the HepG2 cells and promoted apoptosis. Both qRT-PCR and immunofluorescence staining revealed that berberine reduced the NF-kB p65 levels in More >

Jianpeng Liu^*, Wei Li^†, Shunshun Liu^*, Xu Zheng^*, Lin Shi^*, Weitao Zhang^*, Hongfa Yang^*

Oncology Research, Vol.25, No.2, pp. 225-232, 2017, DOI:10.3727/096504016X14732772150587

Abstract Collagen triple helix repeat containing 1 (CTHRC1), an extracellular matrix-related protein, has been found to be upregulated in many solid tumors and contributes to tumorigenesis. We found that CTHRC1 is overexpressed in glioblastoma tissues and cells. By using the technique of RNA interference, the expression of CTHRC1 in the human glioblastoma U-87MG cell line was downregulated, and the proliferation and migration of U-87MG cells were examined. The results showed that the knockdown of CTHRC1 exerts inhibitory effects on the proliferation and migration ability of U-87MG cells. Knockdown of CTHRC1 expression in U-87MG cells resulted in More >

Xiangrui Meng^*1, Xiaoqi Chen^†1, Peng Lu^‡, Wang Ma^*, Dongli Yue^*, Lijie Song^*, Qingxia Fan^*

Oncology Research, Vol.25, No.2, pp. 215-223, 2017, DOI:10.3727/096504016X14732772150541

Abstract Esophageal squamous cell carcinoma (ESCC) is one of the most common malignant cancers with high mortality around the world. However, the regulatory mechanism of ESCC carcinogenesis is not completely known. Here we demonstrate the novel role of miR-202 in regulating ESCC cell apoptosis. The analysis of data obtained from the GEO database showed that the expression of miR-202 is aberrantly decreased in tumor tissue from ESCC patients and cultured ESCC cell lines. After transfection with miR-202 mimic or inhibitor, the apoptotic capacity of ESCC cells was significantly increased by miR-202 overexpression but reduced by miR-202… More >

Ziliang Zhou^*†1, Lijie Zhou^*‡1, Fangfang Jiang^*, Binghui Zeng^*, Changbo Wei^*, Wei Zhao^*, Dongsheng Yu^*

Oncology Research, Vol.25, No.2, pp. 207-214, 2017, DOI:10.3727/096504016X14732772150460

Abstract Previous studies have shown that miR-222 targets the p53 upregulated modulator of apoptosis (PUMA) to regulate cell biological behavior in some human malignancies. We hypothesized that there was a negative regulation, which might induce apoptosis, between miR-222 and PUMA in adenoid cystic carcinoma (ACC). In this study, the expression levels of miR-222 and the PUMA gene after transfection with antisense miR-222 (As-miR-222) were evaluated by RT-PCR and Western blot assays. Cell proliferation and migratory abilities were detected by CCK-8 and Transwell assays. Cell cycle and apoptosis were analyzed by flow cytometry. Our results showed that, More >

Chun-ming Yang^*, Shan Ji^†, Yan Li^‡, Li-ye Fu^‡, Tao Jiang^‡, Fan-dong Meng^‡

Oncology Research, Vol.25, No.2, pp. 195-205, 2017, DOI:10.3727/096504016X14732772150424

Abstract Renal cell carcinoma (RCC) represents one of the most resistant tumors to radiation and chemotherapy. Current therapies for RCC patients are inefficient due to the lack of diagnostic and therapeutic markers. The expression of novel tumor-associated kinases has the potential to dramatically shape tumor cell behavior. Identifying tumor-associated kinases can lend insight into patterns of tumor growth and characteristics. In the present study, we investigated the receptor tyrosine kinase-like orphan receptor 2 (Ror2), a new tumor-associated kinase, in RCC primary tumors and cell lines. Knockdown of Ror2 expression in RCC cells with specific shRNA significantly More >

Haiyang Wang^*, Chongyang Tang^*, Meng Na^*, Wei Ma^*, Zhenfeng Jiang^*, Yifei Gu^*, Guizhen Ma^†, Haitao Ge^*, Hong Shen^*, Zhiguo Lin^*

Oncology Research, Vol.25, No.2, pp. 187-194, 2017, DOI:10.3727/096504016X14732772150389

Abstract Glioma is a common type of malignant brain tumor characterized by aggressive metastasis capability. Recent evidence has suggested that noncoding RNAs, including microRNAs, have important functions in the pathophysiology of glioma development. In this study, we investigated the biological function of miR-422a in human glioma. We found that miR-422a was downregulated in glioma tissues. We also demonstrated that expression of miR-422a in glioma cells markedly suppressed cell proliferation, migration, and invasion. In addition, we identified insulin-like growth factor 1 (IGF1) and IGF1 receptor (IGF1R) as inhibitory targets of miR-422a in glioma cells. We established that More >

Hongsheng Dang, Wuzhou Wu, Bo Wang, Cao Cui, Juwei Niu, Jie Chen, Ziqiu Chen, Yi Liu

Oncology Research, Vol.25, No.2, pp. 177-186, 2017, DOI:10.3727/096504016X14732772150343

Abstract CXCL5, a CXC-type chemokine, is an important attractant for granulocytic immune cells by binding to its receptor CXCR2. Recently, CXCL5/CXCR2 has been found to play an oncogenic role in many human cancers. However, the exact role of CXCL5 in osteosarcoma cell migration and invasion has not been revealed. Here we found that the protein expression of CXCL5 was significantly increased in osteosarcoma tissues compared with that in matched adjacent nontumor tissues. Moreover, the expression of CXCL5 was significantly associated with advanced clinical stage and metastasis. Further investigation showed that the CXCL5 expression levels were also… More >

Jie Huang^*, Li Guo^†

Oncology Research, Vol.25, No.2, pp. 167-176, 2017, DOI:10.3727/096504016X14732772150307

Abstract Sex-determining region Y (SRY)-box 9 (SOX9) is a member of the SOX transcription factor family. Increasing evidence has reported that SOX9 plays different roles in various types of malignancies. However, the role of SOX9 in papillary thyroid cancer (PTC) is still unclear. The aim of this study was to investigate the role of SOX9 in PTC. Our results showed that SOX9 was upregulated in PTC tissues and cell lines. In addition, knockdown of SOX9 significantly inhibited PTC proliferation, colony formation, migration, and invasion, as well as epithelial–mesenchymal transition (EMT) phenotype in TPC-1 and BCPAP cells. More >

Xiangfei He, Fuguang Sun, Fengfu Guo, Kai Wang, Yisheng Gao, Yanfei Feng, Bin Song, Wenzhi Li, Yang Li

Oncology Research, Vol.25, No.2, pp. 157-166, 2017, DOI:10.3727/096504016X14719078133203

Abstract Renal cell carcinoma (RCC) is one of the most common kidney cancers worldwide. Although great progressions have been made in the past decades, its morbidity and lethality remain increasing. Long noncoding RNAs (lncRNAs) are demonstrated to play significant roles in the tumorigenesis. This study aimed to investigate the detailed roles of lncRNA FTX in RCC cell proliferation and metastasis. Our results showed that the transcript levels of FTX in both clinical RCC tissues and the cultured RCC cells were significantly upregulated and associated with multiple clinical parameters of RCC patients, including familial status, tumor sizes, More >