BIOCELL, Vol.39, Suppl.S, pp. 1-2, 2015

Abstract This article has no abstract. More >

Orlando J. CASTEJÓN^*

BIOCELL, Vol.39, No.2-3, pp. 33-40, 2015, DOI:10.32604/biocell.2015.39.033

Abstract Twenty one days old Swiss albino mice that received diphenylhydantoin (25 mg/kg, i.p., daily for 15 days) progressively developed gait alterations, changes of behavior and cerebellar ataxia. Cerebellar slices were processed by conventional transmission electron microscopy. The body of Purkinje cells exhibited fragmented limiting plasma membranes, dilated nuclear envelopes, swelling and disassembly of nuclear pores, enlargement of rough and smooth endoplasmic reticulum and a notable detachment of membrane associated ribosomes, together with distorted vacuoles of smooth endoplasmic reticulum, bizarre shaped and swollen mitochondria with dilated cristae, as well as disrupted limiting lysosomal membranes. Degenerated… More >

Weiyu FU¹, Hongyu LU², Li LI³, Kefeng WU³, Yanping LI³, Yi LIU^3,*, Jianli HU^4,*

BIOCELL, Vol.39, No.2-3, pp. 25-32, 2015, DOI:10.32604/biocell.2015.39.025

Abstract Objective: To assess the diagnostic accuracy of serum GPC3 versus alpha-fetoprotein (AFP) for HCC by using the method of system review.
Methods: PubMed and EMBASE were searched from its inception to 20, April 2014 for studies that compared diagnostic accuracy of serum GPC3 with AFP for HCC. Sensitivity, specificity and other measures were pooled using random-effects models. Summary receiver operating characteristic curve analysis was used to summarize the overall test performance.
Results: Fourteen studies were included in this meta-analysis. Summary estimates for serum GPC3 and AFP in diagnosing HCC were as follows: sensitivity, 69% (95% confidence… More >

Gonzalo RODRÍGUEZ-LOMBARDI¹, Luciana REYNA², María Susana VITALI¹, Susana GENTI-RAIMONDI²

BIOCELL, Vol.39, No.2-3, pp. 15-24, 2015, DOI:10.32604/biocell.2015.39.015

Abstract A competitive internal control (IC) adapted to RT-PCR in-house assay was developed for HCV RNA detection in human pooled plasma. Also, it was applied in a multiplex RT-PCR for the HIV-1 and HCV RNA screening in human pooled plasma and plasma-derived products. A 258-bp PCR product from the 5´noncoding region of HCV genome was obtained. A competitive IC template was constructed by inserting a 52-bp double strand sequence into the NheI site of the 258-bp amplicon. This sequence was cloned and the obtained plasmid was used to generate a synthetic RNA. The IC/RNA was incorporated… More >

Anna PAŃCZYSZYN^{1 *}, Anna KROP-WATOREK^1,2, Maciej WIECZOREK¹

BIOCELL, Vol.39, No.2-3, pp. 1-4, 2015, DOI:10.32604/biocell.2015.39.001

Abstract Carcinoembryonic antigen (CEA) is a surface glycoprotein expressed in human epithelial cells and is released from their surface, especially during colorectal cancer. Frequently, colorectal cancer is accompanied by inflammation, where tumor-infiltrating neutrophils play an important role. CEA was also found to be a strong chemotactic agent for neutrophils. The purpose of this study was to find out if CEA can enhance neutrophil priming and activation. Primed neutrophils were activated by N-formyl-methionyl-leucyl-phenylalanine (formyl-MLP) and the resulting oxidative burst was measured luminometrically. Unexpectedly, in vitro priming of neutrophils by CEA, alone or preceded by LPS, inhibited subsequent More >

Odin RAMÍREZ-FERNÁNDEZ^1,*, Rafael GODÍNEZ¹, Esmeralda ZUÑIGA-AGUILAR¹, Luis E. GÓMEZ-QUIROZ², María C. GUTIÉRREZ-RUIZ², Juan MORALES³, Roberto OLAYO³

BIOCELL, Vol.39, No.2-3, pp. 9-14, 2015, DOI:10.32604/biocell.2015.39.009

Abstract We have designed and evaluated a radial-flow bioreactor for three-dimensional liver carcinoma cell culture on a new porous coated scaffold. We designed a culture chamber where a radial flow of culture medium was continuously delivered through it. Once this system was established, flow was simulated using flow dynamics software based on numeric methods to solve Navier-Stockes flow equations. Perfusion within cell culture scaffolds was simulated using a flow velocity of 7 mL/min and found that cell culture medium was distributed unhindered in the bioreactor chamber. Afterwards, the bioreactor was built according to the simulated design More >

Rafael Martins de OLIVEIRA, Carolina Bernardi STEFANI, Angélica Aparecida Antoniellis SILVEIRA, Marcelo Lima RIBEIRO, José PEDRAZZOLI Jr., Alessandra GAMBERO^*

BIOCELL, Vol.39, No.2-3, pp. 5-8, 2015, DOI:10.32604/biocell.2015.39.005

Abstract Adenosine acts as a gastroprotective factor decreasing inflammation and reducing gastric acid secretion. Quantitative RT-PCR was used to determine the expression of the adenosine receptor genes (A1AR, A2AAR, A2BAR and A3AR) and that of the gastrin receptor B gene (CCKBR) in isolated, short-term cultured enterochromaffin-like (ECL) cells. Both the A1AR and the CCKBR genes were expressed at a level higher than the other genes. Also, the effect of 2-chloroadenosine, a stable agonist of A1 and A2A receptors, was explored on ECL cells, with a resulting inhibition of both basal and gastrin-stimulated histamine release. Also, dipropylcyclopentylxanthine (DPCPX), a More >

B. Šavija¹, E. Schlangen¹

CMC-Computers, Materials & Continua, Vol.48, No.3, pp. 181-202, 2015, DOI:10.3970/cmc.2015.048.181

Abstract In this paper, a novel approach for simulating in-plane vibration of thin plates is proposed. It is based on the spectral element method (SEM) used within a lattice modeling framework. First, derivation of a frequency dependent dynamic stiffness matrix for a spectral beam element is presented. Then, the lattice modeling concept is introduced. In the model, the two-dimensional plate is discretized as a set of (one dimensional) spectral beam elements connected at the ends. The proposed approach is then used for modal analysis of rectangular plates of different aspect ratios (1 and 2) and boundary More >

Zhengzheng Cao¹, Yuejin Zhou^1,2

CMC-Computers, Materials & Continua, Vol.48, No.2, pp. 77-90, 2015, DOI:10.3970/cmc.2015.048.077

Abstract Reasonable width design of narrow coal pillar is the key for ensuring stability of surrounding rock in the roadway driving along next goaf. Based on movement characteristics of overlying strata in gob-side entry, the paper establishes the mechanical model of surrounding rock structure for roadway driving along next goaf, and analyzes the interaction mechanism between key blocks and coal pillar. Besides, in allusion to the mechanical equilibrium of key block B in basic roof, the stability coefficients K₁ and K₂ of key block B and sensitive coefficient of influencing factor are introduced. When the stability coefficient K₁ is… More >

Cao Zhengzheng¹, Zhou Yuejin^1,2, Zhang Qi¹, Wang Erqian¹

CMC-Computers, Materials & Continua, Vol.47, No.3, pp. 203-215, 2015, DOI:10.3970/cmc.2015.047.203

Abstract Gas fracturing technology is the key to the exploration for unconventional petroleum resources and other engineering industries, so the research on the coupled gas-solid-thermal model during rock damage has the important significance to the development of gas fracturing technology. By introducing rock damage variable, the coupled gas-solid-thermal model during rock damage is established in this paper, besides, the rock damage constitutive is written with MATLAB software, which is embedded in the multi-physics coupling software COMSOL in the process of numerical computation. Based on this, the damage rule of rock mass around drilling under high pressure… More >