ARTURO D. JORGE^*, LUIS S. GUTIERREZ^**, OLIVER JORGE^*, MARIO H. BURGOS^**

BIOCELL, Vol.26, No.2, pp. 253-262, 2002, DOI:10.32604/biocell.2002.26.253

Abstract The aim of this paper is to establish whether there are cytochemical or ultrastructural alterations in the hepatocytes of patients with primary biliary cirrhosis (PBC) at stages I and II compared with the biopsies from individuals with normal liver. Cytochemical technique with ATP as substrate, transmission electron microscopy (TEM) and freeze fracture were used for the studies.
In the normal liver biopsies the ultrastructural cytochemical localization of the enzymatic activity was clearly shown in the bile canaliculi. In the PBC biopsies, the enzymatic activity is increased in the bile canaliculi and is also present in the lateral membranes of… More >

Analia G. Karadayian¹, Juanita Bustamante², Silvia Lores-Arnaiz^{1, *}

BIOCELL, Vol.40, No.1, pp. 31-34, 2016, DOI:10.32604/biocell.2016.40.031

Abstract Alcohol hangover (AH) is defined as the temporary state after alcohol binge-like drinking, starting when EtOH is absent in plasma. Results from our laboratory have shown behavioral impairments and mitochondrial dysfunction in an experimental model of AH in mice. Our model consisted in a single i.p. injection of EtOH (3.8 g/kg BW) or saline solution in male and female mice, sacrificing the animals 6 hours after injection. Motor and affective behavior together with mitochondrial function and free radical production were evaluated in brain cortex and cerebellum during AH. Results showed that hangover animals exhibited a significant reduction in neuromuscular coordination,… More >

MARÍA TERESA PONCE, MÓNICA ELIZABETH GUIÑAZÚ, RICARDO TIZIO

BIOCELL, Vol.26, No.2, pp. 263-266, 2002, DOI:10.32604/biocell.2002.26.263

Abstract The goal of this study was to determine the effect of putrescine, added to the culture medium, on the in vitro development of stenospermic grape (Vitis vinifera L) embryos. The cross breedings of Perlón x G.C88552 and Perlón x Argentina were used. 0 (control), 2 and 4 mM of putrescine were added to the immature seed’s culture medium. In Perlón x Argentina, 2mM of putrescine statistically increased the percentage of total embryos, direct germination, polyembryos and normal plants. In Perlón x G.C88552, only 2 mM of putrescine increased all the variables considered, eventually tripling the percentage of normal plants obtained.… More >

Afet Golayoğlu Fatullayev¹, Canan Köroğlu²

CMES-Computer Modeling in Engineering & Sciences, Vol.86, No.1, pp. 39-52, 2012, DOI:10.3970/cmes.2012.086.039

Abstract In this work we solve numerically a boundary value problem for second order fuzzy differential equations under generalized differentiability in the form y''(t) = p(t)y'(t) + q(t)y(t) + F(t) y(0) = γ, y(l) = λ where t ∈T = [0,l], p(t)≥0, q(t)≥0 are continuous functions on [0,l] and [γ]^α = [γ_{_α},γ⁻_α], [λ]^α = [λ_{_α},λ^¯_α] are fuzzy numbers. There are four different solutions of the problem (0.1) when the fuzzy derivative is considered as generalization of the H-derivative. An algorithm is presented and the finite difference method is used for solving obtained problems. The applicability of presented… More >

Darío E. IGLESIAS^*, Silvina S. BOMBICINO, Alberto BOVERIS, Laura B. VALDEZ

BIOCELL, Vol.40, No.1, pp. 27-30, 2016, DOI:10.32604/biocell.2016.40.027

Abstract Complex III plays a central role in the mitochondrial respiratory chain transferring electrons from ubiquinol to cytochrome c and pumping protons to the intermembrane space, contributing to the protonmotive force. Furthermore, complex III can act as a source of O₂ •- in the presence of ubiquinol and antimycin, an expermiental condition in which the oxidation of the cytochrome b hemes is blocked. The O₂ •- dismutation catalyzed by superoxide dismutase produces H₂O₂, a known second messenger in redox signalling. Results from our laboratory have shown that NO, released from GSNO or from SPER -NO or generated by mtNOS, inhibits electron… More >

VÍCTOR PANZA^1,2, VERÓNICA LÁINEZ² , SARA MALDONADO^1,2, HORACIO L. MARODER³

BIOCELL, Vol.31, No.3, pp. 383-390, 2007, DOI:10.32604/biocell.2007.31.383

Abstract Information on desiccation sensitivity of Euterpe edulis seeds under two drying rates is presented. The sensitivity was studied during the course of germination and normal germination. The water content was evaluated for both seeds and embryos. Results showed the following: (a) For both drying treatments and for both germination and normal germination, desiccation sensitivity values were higher for measurements based on the water content of the embryo than for those of the seed. (b) For both drying treatments, desiccation sensitivity were higher for normal germination than for germination based on both the embryo and seed water contents. (c) Under the… More >

Marcelo HERNANDO¹, Christian HOUGHTON¹, Leda GIANNUZZI², Bernd KROCK³, Darío ANDRINOLO², Gabriela MALANGA^4,*

BIOCELL, Vol.40, No.1, pp. 23-26, 2016, DOI:10.32604/biocell.2016.40.023

Abstract Cyanobacteria are phototrophic organisms with great ecological and economical importance. Species of the genus Microcystis are known for their potential ability to synthesize toxins, notably microcystins. There is a growing interest in the evaluation of oxidative stress in relation to the impact of global climate change on natural ecosystems in different trophic levels. Several studies have focused on the analysis of organismal responses to mitigate the damage by controlling the generation of reactive oxygen species. Variations in environmental factors caused by climate change generate a situation of oxidative damage in Microcystis aeruginosa as a direct or indirect consequence. In this… More >

S. NICOVANI , M.I. RUDOLPH

BIOCELL, Vol.26, No.1, pp. 15-24, 2002, DOI:10.32604/biocell.2002.26.015

Abstract We examined the presence of estrogen receptors (ER) in vascular mast cells and a possible genomic effect of estrogens on the expression of mast cell (MC) mediators such as chymase, TNFα, NOS and IL-10, which are known to affect the course of atherosclerosis. Immunocytochemical detection of mast cell tryptase and the co-localization of ERs in MCs from abdominal aortic vessels from 10 fertile woman, 10 postmenopausal women and 15 men was performed. The genomic expression of IL-10, TNFα, and NOS was analyzed by RT-PCR and chymase activity by spectrophotometry after 24 h incubation with 17-β estradiol (0.2-0.5 ng/mL) in rat… More >

L.A. CARO¹, P.A. POLCI¹ , L.I. LINDSTRÖM¹ , C.V. ECHENIQUE^1,2, L.F. HERNÁNDEZ^1,3

BIOCELL, Vol.26, No.1, pp. 25-33, 2002, DOI:10.32604/biocell.2002.26.025

Abstract Prosopis chilensis (Mol.) Stuntz (Algarrobo de Chile) is an important native tree species that can be grown in arid and semiarid regions for wood and forage production and environmental protection. Developing a simple and reliable in vitro protocol for cloning it would enable to improve it genetically. Explants of P.chilensis were taken from 4 months-old plants grown in the greenhouse or from adult trees grown in a natural environment. Nodal segments 1 – 2 cm long containing an axillary bud were selected from elongating shoots. These cuttings were aseptically cultured on two agar-solid basal media, MS or BTMm, and treated… More >

PAOLA GAUFFIN CANO¹ , GRACIELA AGÜERO³, GABRIELA PERDIGON^1,2

BIOCELL, Vol.26, No.1, pp. 35-48, 2002, DOI:10.32604/biocell.2002.26.035

Abstract Nutritional deficiencies are associated with impaired immune response, affecting the body’s defence mechanisms. It is also known that Lactic Acid Bacteria (LAB) and fermented products such us yogurt have immunopotentiator activity and nutritional properties, and could thus be used as a valuable supplement in a renutrition diet. The aim of this study was to determine, in a non-severe malnutrition model, the effective dose of Lactobacillus casei (L.casei), which when is used as an adjuvant in a renutrition diet, would modulate the mucosal immune system and induce recovery of the integrity of the intestinal barrier. The experiments were performed on groups… More >