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  • Open Access

    ARTICLE

    Enhancement of Drug Sensitivity by Knockdown of HIF-1α in Gastric Carcinoma Cells

    Qun Zhao, Bi-Bo Tan, Yong Li, Li-Qiao Fan, Pei-Gang Yang, Yuan Tian

    Oncology Research, Vol.23, No.3, pp. 129-136, 2015, DOI:10.3727/096504015X14500513118029

    Abstract In this study, the effects of hypoxia-inducible factor-1α (HIF-1α) on gastric carcinoma (GC) drug resistance through apoptosis-related genes are investigated. First, HIF-1α-specific siRNA was synthetized and transfected into drug-resistant GC cell line OCUM-2MD3/L-OHP. Then MTT assay was applied to test the inhibition rate of GC cells by 5-fluorouracil (5-FU) and oxaliplatin (L-OHP). After that, flow cytometry (FCM) was applied to measure apoptosis rate. qPCR and Western blot assay were employed to detect HIF-1α and apoptosis-related genes. Results showed that HIF-1α in OCUM-2MD3/L-OHP cells was higher than that in OCUM-2MD3 and gastric epithelial cells. After HIF-1α-siRNA More >

  • Open Access

    ARTICLE

    HSP27 Knockdown Increases Cytoplasmic p21 and Cisplatin Sensitivity in Ovarian Carcinoma Cells

    Hao Lu1, Chaoyang Sun1, Ting Zhou, Bo Zhou, Ensong Guo, Wanying Shan, Meng Xia, Kezhen Li, Danhui Weng, Li Meng, Xiaoyan Xu, Junbo Hu, Ding Ma, Gang Chen

    Oncology Research, Vol.23, No.3, pp. 119-128, 2015, DOI:10.3727/096504015X14496932933656

    Abstract Drug resistance is the leading cause of chemotherapy failure in the treatment of ovarian cancer. So far, little is known about the mechanism of chemoresistance in ovarian cancer. In this study, we explored the mechanism that HSP27 was involved in cisplatin resistance of ovarian cancer both in vitro and clinically. HSP27 protein was found to be upregulated and expressed in cisplatin-resistant ovarian cancer cell line C13*, and HSP27 siRNA transfection reversed the chemoresistance of C13*. We found that HSP27 exerted its chemoresistant role by inhibiting p21 transferring from the nucleus to the plasma through the More >

  • Open Access

    ARTICLE

    ERK Signaling Pathway Is Involved in HPV-16 E6 but not E7 Oncoprotein-Induced HIF-1α Protein Accumulation in NSCLC Cells

    Fei Liu*1, Bihua Lin*1, Xin Liu*, Wenzhang Zhang*, Erying Zhang*, Liang Hu*, Yuefan Ma*, Xiangyong Li*, Xudong Tang*†

    Oncology Research, Vol.23, No.3, pp. 109-118, 2015, DOI:10.3727/096504015X14496932933610

    Abstract Extracellular signal-regulated kinase (ERK)1/2 signaling pathway plays a critical role in regulating tumor angiogenesis. Our previous studies have demonstrated that HPV-16 oncoproteins enhanced hypoxia-inducible factor-1α (HIF-1α) protein accumulation and vascular endothelial growth factor (VEGF) and interleukin-8 (IL-8) expression in non-small cell lung cancer (NSCLC) cells, thus contributing to angiogenesis. In this study, we further investigated the role of ERK1/2 signaling pathway in HPV-16 oncoprotein-induced HIF-1α, VEGF, and IL-8 expression and in vitro angiogenesis in NSCLC cells. Our results showed that HPV-16 E6 and HPV-16 E7 oncoproteins promoted the activation of ERK1/2 signaling pathway in A549 More >

  • Open Access

    ARTICLE

    Long Noncoding RNA H19-Derived miR-675 Enhances Proliferation and Invasion via RUNX1 in Gastric Cancer Cells

    Gao Liu*, Tian Xiang, Quan-Feng Wu, Wei-Xing Wang*

    Oncology Research, Vol.23, No.3, pp. 99-107, 2015, DOI:10.3727/096504015X14496932933575

    Abstract The lncRNA H19 and its mature product miR-675 have recently been shown to be upregulated and promote the progression of gastric cancer. However, the detailed function and underlying molecular mechanism of H19/miR- 675 in the carcinogenesis of gastric cancer remains unclear. In this study, we found that H19 depended on miR- 675 to enhance the proliferation and invasion of gastric cancer AGS cells, and the expression of miR-675 was positively correlated with H19 in patients with gastric cancer. Subsequently, the tumor-suppressor runt domain transcription factor 1 (RUNX1) was confirmed to be a downstream molecule of… More >

  • Open Access

    ARTICLE

    Inhibition of Migration and Invasion by Tet-1 Overexpression in Human Lung Carcinoma H460 Cells

    Si Jun Park*1, Bo Ram Lee*1, Hyeng-Soo Kim*, Young Rae Ji*, Yong Hun Sung*, Kwang ShikChoi*, Hum Dai Park, Sung-Hyun Kim*, Myoung Ok Kim, Zae Young Ryoo*

    Oncology Research, Vol.23, No.3, pp. 89-98, 2015, DOI:10.3727/096504015X14496932933539

    Abstract In the present study, we found that lung cancer cell line (H460 cells) expressing Tet1 showed higher levels of adhesion, and Tet1 inhibited H460 cell proliferation. In addition, these cells showed a significantly reduced ability of collagen degradation and Smad2/3 phosphorylation compared to controls. Furthermore, vimentin was found to be highly expressed in larger metastatic cancer area. Tet1 overexpression was reduced in the epithelial marker E-cadherin. Moreover, Tet1 repressed cancer cell metastasis in nude mice. Collectively, these findings suggest that Tet1 expression plays a critical role in metastasis of lung cancer cells by suppression of More >

  • Open Access

    ARTICLE

    FGF19 Contributes to Tumor Progression in Gastric Cancer by Promoting Migration and Invasion

    Shuang Wang*, Daqi Zhao, Ruihua Tian*, Hailong Shi*, Xiangming Chen*, Wenzhi Liu*, Lin Wei*

    Oncology Research, Vol.23, No.4, pp. 197-203, 2015, DOI:10.3727/096504016X14537290676919

    Abstract Gastric cancer is the fourth most common type of cancer and second leading cause of cancer-related death in the world. Since patients are often diagnosed at a late stage, very few effective therapies are left in the arsenal. FGF19, as a hormone, has been reported to promote tumor growth in various types of cancer; however, its function in gastric cancer remains unknown. In the current study, we showed that FGF19 is overexpressed in gastric cancer and is associated with depth of invasion, lymph node metastasis, and TNM stage. In addition, in vitro experiments demonstrated that More >

  • Open Access

    ARTICLE

    Clinical Outcome in Definitive Concurrent Chemoradiation With Weekly Paclitaxel and Carboplatin for Locally Advanced Esophageal and Junctional Cancer

    Vanita Noronha*, Kumar Prabhash*, Amit Joshi*, Vijay Maruti Patil*, Sanjay Talole, Dipti Nakti*, Arvind Sahu*, Srushti Shah, Sarbani Ghosh-Laskar§, Prachi S. Patil, Shaesta A. Mehta, Nirmala Jambhekar#, Abhishek Mahajan**, Nilendu Purandare††

    Oncology Research, Vol.23, No.4, pp. 183-195, 2015, DOI:10.3727/096504016X14537290676865

    Abstract There are little data on the efficacy and safety of taxane/platinum with definitive radiotherapy (RT) for esophageal/GEJ cancer. This article is a retrospective analysis of patients who received weekly paclitaxel 50 mg/ m2 and carboplatin AUC 2 with radical definitive RT for locally advanced esophageal/GEJ cancer. Between February 2011 and July 2014, 179 patients were included. The median age was 54 years. Ninety-two percent of patients had squamous histology. Mean RT dose was 58.7 Gy in 32 fractions over 53 days, with mean of six chemotherapy cycles. Fifty-six percent of patients developed ³grade 3 acute… More >

  • Open Access

    ARTICLE

    Inhibition of Liver Carcinoma Cell Invasion and Metastasis by Knockdown of Cullin7 In Vitro and In Vivo

    Donghui Zhang*1, Genling Yang†1, Xidong Li, Cheng Xu*, Honglei Ge§

    Oncology Research, Vol.23, No.4, pp. 171-181, 2015, DOI:10.3727/096504016X14519995067562

    Abstract Cullin7 is an E3 ubiquitin ligase. The Cullin7 protein family functions as a molecular scaffold to coordinate substrate ubiquitination in Skp, Cullin, and F-box-containing complex (SCF complex). Cullin7s control normal development and primary cellular processes and are characterized by a unique genomic network organization. Less is known about the involvement of Cullin7 with hepatocellular carcinoma (HCC). In this study, we found that Cullin7 showed a high expression in HCC tumor tissues, especially in metastatic HCC tumor tissues. Also, there was a negative correlation between Cullin7 expression and long survival. Silencing of Cullin7 in liver cancer More >

  • Open Access

    ARTICLE

    miR-544a Promotes Breast Cancer Cell Migration and Invasion Reducing Cadherin 1 Expression

    Pengwei Lu, Yuanting Gu, Lin Li, Fang Wang, Xinguang Qiu

    Oncology Research, Vol.23, No.4, pp. 165-170, 2015, DOI:10.3727/096504016X14519157902726

    Abstract Accumulating evidence has reported the significant role of miRNAs in the underlying biology of tumors, including breast cancer. The purpose for this study was to investigate the potential effects of miR-544a in breast cancer migration and invasion. The human normal breast Hs578Bst cells and the human breast cancer MCF-7 and MDA-MB-231 cells were used to analyze the expression of miR-544a by RT-PCR. The effects of miR-544a on the two kinds of breast cancer cell migration and invasion were analyzed using the Matrigel and Transwell assay, respectively. miR-544a expression on the cell metastasis-related protein expression was More >

  • Open Access

    ARTICLE

    miRNA-497 Negatively Regulates the Growth and Motility of Chondrosarcoma Cells by Targeting Cdc25A

    Yandong Lu*1, Fangguo Li*1, Tao Xu, Jie Sun*1

    Oncology Research, Vol.23, No.4, pp. 155-163, 2015, DOI:10.3727/096504016X14519157902681

    Abstract Chondrosarcoma (CHS) is the second most common malignant bone sarcoma with increased risk of invasion and metastasis. However, the regulatory mechanisms of CHS tumorigenesis remain unknown. Here we investigated the novel role of miR-497 in regulating chondrosarcoma cell growth and cell cycle arrest. RT-PCR analysis showed that the expression of miR-497 is aberrantly downregulated in human chondrosarcoma samples and cells. After transfection with miR-497 mimic or antagomir, the proliferation and apoptosis of JJ012 and OUMS-27 chondrosarcoma cells were determined by CCK-8 assay and flow cytometric analysis, respectively. Results showed that the proliferation capacity of JJ012… More >

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