Erkun Guo, Hongjiang Liu, Xiaopeng Liu

Oncology Research, Vol.25, No.3, pp. 437-444, 2017, DOI:10.3727/096504016X14747335734344

Abstract Signal peptide CUB EGF-like domain-containing protein 2 (SCUBE2), a member of the SCUBE family of proteins, was recently found to play an important role in cancer development. However, little is known regarding its biological function in glioma. In the present study, we investigated the effect of SCUBE2 on glioma and explored its relevant mechanisms. The study showed that SCUBE2 had a low expression in glioma tissue and cell lines. SCUBE2 overexpression inhibited glioma cell proliferation in vitro and in vivo as well as suppressed glioma cell migration and invasion in vitro. Furthermore, we found that More >

Xiaorui Liu^*†1, Jingjing Li^*1, Zujiang Yu^*, Juan Li^*, Ranran Sun^*, Quancheng Kan^‡

Oncology Research, Vol.25, No.3, pp. 427-435, 2017, DOI:10.3727/096504016X14747300207374

Abstract Hepatocellular carcinoma (HCC) is the most common cancer in the world. MicroRNAs (miRNAs) are a type of small noncoding RNA that can regulate the expression of target genes under physiological and pathophysiological conditions. Aberrant expression of microRNA-935 (miR-935) has been reported in cancer studies. However, its expression and mechanism in HCC remain unclear. In our study, we found that miR-935 was upregulated in liver cancer tissues and cells. Overexpression of miR-935 in liver cells promoted cell proliferation, tumorigenesis, and cell cycle progression, whereas inhibition of miR-935 reduced cell proliferation, tumorigenicity, and cell cycle progression. These… More >

Feng Jiang¹, Dengfeng Zhang¹, Guojun Li, Xiao Wang

Oncology Research, Vol.25, No.3, pp. 417-425, 2017, DOI:10.3727/096504016X14747253292210

Abstract DDX46, a member of the DEAD-box (DDX) helicase family, is involved in the development of several tumors. However, the exact role of DDX46 in osteosarcoma and the underlying mechanisms in tumorigenesis remain poorly understood. Thus, in the present study, we explored the role of DDX46 in osteosarcoma and the underlying mechanisms. Our results demonstrated that the expression levels of DDX46 in both mRNA and protein were greatly elevated in human osteosarcoma tissues and cell lines. Knockdown of DDX46 obviously inhibited osteosarcoma cell proliferation and tumor growth in vivo. In addition, knockdown of DDX46 also significantly More >

Cheng Xu^*1, Lu Cao^*1, Jianhua Liu^†1, Zhongrun Qian^‡, Yu Peng^§, Wenjing Zhu^§, Yongming Qiu^‡, Yingying Lin^‡¶

Oncology Research, Vol.25, No.3, pp. 407-415, 2017, DOI:10.3727/096504016X14743350548249

Abstract Elevated circulating asparaginyl endopeptidase (AEP), a novel lysosomal protease, has been found in breast cancer, and AEP is thus considered to be a prognostic factor in this disease. However, the pathological functions of circulating AEP in the development of breast cancer and the potential of AEP-targeted therapy remain unclear. We used MMTV-PyVmT transgenic mice, which spontaneously develop mammary tumors. Western blotting showed overexpression of AEP in both primary tumor tissue and lung metastases compared to their normal counterparts. Moreover, the concentration of circulating AEP gradually increased in the serum during the development of mammary tumors. More >

Yong Zhou, Chuandong Yang, Kunpeng Wang, Xuefeng Liu, Quan Liu

Oncology Research, Vol.25, No.3, pp. 397-405, 2017, DOI:10.3727/096504016X14743337535446

Abstract Recently, microRNA (miR)-33b has been demonstrated to act as a tumor suppressor in osteosarcoma. However, the regulatory mechanism of miR-33b in osteosarcoma cell proliferation and migration remains largely unknown. In this study, real-time PCR showed that miR-33b was significantly downregulated in osteosarcoma tissues compared to their matched adjacent nontumor tissues. Its expression was also decreased in several common osteosarcoma cell lines, including Saos-2, MG63, U2OS, and SW1353, when compared to normal osteoblast cell line hFOB. Overexpression of miR-33b suppressed U2OS cell proliferation and migration. HIF-1α was further identified as a target of miR-33b, and its… More >

Xingtao Han^*†, Jinjian Yang^*, Zhankui Jia^*, Pengtao Wei^†, Han Zhang^†, Wenwei Lv^†, Jiantao Sun^†, Qingxiang Huo^†

Oncology Research, Vol.25, No.3, pp. 389-395, 2017, DOI:10.3727/096504016X14743324568129

Abstract The pre-mRNA splicing regulator serine–arginine protein kinase 1 (SRPK1), a member of the SR kinase family, plays an essential role in cancer development and various pathophysiological processes. However, its expression pattern and functions in renal cell carcinoma (RCC) remain unknown. Therefore, the aim of this study was to assess the role of SRPK1 in RCC. Our data showed that SRPK1 was significantly upregulated in human RCC tissues and cell lines. SRPK1 interference significantly inhibited the proliferation of RCC cells and inhibited tumor growth in vivo. In addition, SRPK1 interference also suppressed migration and invasion in More >

Gulijiahan Aierken¹, Ayinuer Seyiti¹, Mayinuer Alifu, Gulina Kuerban

Oncology Research, Vol.25, No.3, pp. 381-388, 2017, DOI:10.3727/096504016X14741511303522

Abstract The tripartite motif (TRIM) family of proteins is a class of highly conservative proteins that have been implicated in multiple processes. TRIM59, one member of the TRIM family, has now received recognition as a key regulator in the development and progression of human diseases. However, its role in human tumorigenesis has remained largely unknown. In this study, the effects of TRIM59 expression on cell proliferation and migration were investigated in human cervical cancer cells. The expression of TRIM59 in clinical cervical cancer tissues and cervical cancer cells was initially determined by RT-PCR and Western blot.… More >

Zhao Xiaoguang^*1,2, Liu Meirong^†1, Zhang Jingjing^‡, Zhang Ruishen^§, Zhang Qing^¶, Tan Xiaofeng^†2

Oncology Research, Vol.25, No.3, pp. 373-380, 2017, DOI:10.3727/096504016X14741486659473

Abstract The long noncoding CPS1 intronic transcript 1 (lncRNA CPS1-IT1) is a recently identified tumor suppressor in the lncRNA family of proteins. Whether this lncRNA plays any functional role in solid tumors remains largely unknown. The present study aimed to investigate the role of lncRNA CPS1-IT1 in human lung cancer. Expression of lncRNA CPS1-IT1 was initially assessed in human lung cancer and in a series of lung cancer cell lines. The effects of CPS1-IT1 overexpression on cell proliferation, migration, and invasion were examined in lung cancer cell lines A549 and 95D. It was found that lncRNA… More >

Zhenjiang Li^*1, Chenyang Xu^*1, Ming Gao^*, Bingqian Ding^*, Xinting Wei^†, Nan Ji^‡

Oncology Research, Vol.25, No.3, pp. 365-372, 2017, DOI:10.3727/096504016X14738135889976

Abstract Jumonji AT-rich interactive domain 2 (JARID2) is a member of the Jumonji family of proteins and has been proposed as an oncogene in several types of human cancer. However, the role of JARID2 in human glioma has not yet been understood. The present study was designed to determine the roles of JARID2 in the proliferation and migration in human glioma cells and the growth of glioma cells in nude mice. Our data indicate that JARID2 is upregulated in human glioma tissues and cell lines. Knockdown of JARID2 obviously inhibits the proliferation of U87MG cells and More >

Guojun Wang, Yang Fu, Guanghui Liu, Yanwei Ye, Xiefu Zhang

Oncology Research, Vol.25, No.3, pp. 355-364, 2017, DOI:10.3727/096504016X14738114257367

Abstract MicroRNAs (miRNAs) play an important role in carcinogenesis. miR-218 is one of the most known miRNAs and has been demonstrated to inhibit progression in gastric cancer. However, the underlying molecular mechanism is not established. In this study, qRT-PCR and Western blot indicated that miR-218 was downregulated in gastric cancer cell lines SGC7901 and BGC823 compared to that in normal gastric epithelial cell line GES-1. MTT and wound scratch assays suggested that overexpression of miR-218 markedly suppressed cell proliferation, migration, and EMT of gastric cancer cells. Furthermore, we proved that WASF3 was a direct target of More >