Home / Advanced Search

  • Title/Keywords

  • Author/Affliations

  • Journal

  • Article Type

  • Start Year

  • End Year

Update SearchingClear
  • Articles
  • Online
Search Results (21,909)
  • Open Access

    ARTICLE

    A cryopreservation protocol for immature zygotic embryos of species of Ilex (Aquifoliaceae)

    LUIS A. MROGINSKI*, PEDRO A. SANSBERRO, ADRIANA M. SCOCCHI, CLAUDIA LUNA, HEBE Y. REY

    BIOCELL, Vol.32, No.1, pp. 33-39, 2008, DOI:10.32604/biocell.2008.32.033

    Abstract Tropical Ilex species have recalcitrant seeds. This work describes experiments demonstrating the feasibility of long-term conservation of Ilex brasiliensis, I. brevicuspis, I. dumosa, I. intergerrima, I. paraguariensis, I. pseudoboxus, I. taubertiana, and I. theezans through cryopreservation of zygotic rudimentary embryos at the heart developmental stage. The embryos were aseptically removed from the seeds and precultured (7 days) in the dark, at 27± 2ºC on solidified (0.8% agar) 1/4MS medium, [consisting of quarterstrength salts and vitamins of Murashige and Skoog (1962) medium] with 3% sucrose and 0.1 mg/l Zeatin. The embryos were then encapsulated in 3% calcium alginate beads and pretreated… More >

  • Open Access

    ARTICLE

    Antioxidant capacity of vitamin C in mouse liver and kidney tissues

    ALICIA WEYERS, LAURA I. UGNIA, HUGO GARCÍA OVANDO, NORA B. GORLA*

    BIOCELL, Vol.32, No.1, pp. 27-31, 2008, DOI:10.32604/biocell.2008.32.027

    Abstract In the present study, the antioxidant capacity of vitamin C was examined in the liver and the kidney tissues of mice with or without ciprofloxacin (CFX) treatment. The antioxidant capacity of the vitamin was evaluated in terms of lipid hydroperoxides (LOOH) and thiobarbituric acid reactive substances (TBARs). The experimental design was 15 days of water (control and CFX groups) or vitamin C (vitamin C and vitamin C plus CFX groups) in drinking water. One dose of CFX was injected, 15 minutes before sacrifice, in the corresponding mice.
    The initial nmol of lipid hydroperoxides/g of tissue were 137 ± 11… More >

  • Open Access

    ARTICLE

    Models for the recent evolution of protocadherin gene clusters

    MARCOS MORGAN

    BIOCELL, Vol.32, No.1, pp. 9-26, 2008, DOI:10.32604/biocell.2008.32.009

    Abstract The clustered protocadherins (Pcdhs) are single-pass transmembrane proteins that constitute a subfamily within the cadherin superfamily. In mammals, they are arranged in three consecutive clusters named α, β, and γ. These proteins are expressed in the nervous system and are targeted to mature synapses. Interestingly, different neurons express different subsets of isoforms; however, little is known about the functions and expression of the clustered Pcdhs.
    Previous phylogenetic analyses that compared rodent and human clusters postulated the recent occurrence of gene duplication events. Using standard phylogenetic methods, I confirmed the prior observations, and I show that duplications are likely to… More >

  • Open Access

    REVIEW

    Review : A tridimensional view of the organization of actin filaments in the central nervous system by use of fluorescent photooxidation

    FRANCISCO CAPANI1, EZEQUIEL SARACENO1, VALERIA ROMINA BOTI1, LAURA AON-BERTOLINO1, JUAN CARLOS FERNÁNDEZ1, FERNANDO GATO1, MARIA SOL KRAUSE2, LISANDRO GIRALDEZ3, MARK H. ELLISMAN4, HÉCTOR COIRINI1,2

    BIOCELL, Vol.32, No.1, pp. 1-8, 2008, DOI:10.32604/biocell.2008.32.001

    Abstract Cellular and subcellular organization and distribution of actin filaments have been studied with various techniques. The use of fluorescence photo-oxidation combined with phalloidin conjugates with eosin has allowed the examination of the precise cellular and subcellular location of F-actin. Correlative fluorescence light microscopy and transmission electron microscopy studies of F-actin distribution are facilitated with this method for morphological and physiological studies. Because phalloidin-eosin is smaller than other markers, this method allows the analysis of the three-dimensional location of F-actin with high-resolution light microscopy, three-d serial sections reconstructions, and electron tomography. The combination of selective staining and three-dimensional reconstructions provide a… More >

  • Open Access

    ARTICLE

    Brief Note : Hydrogen peroxide in micropropagation of Lilium. A comparison with a traditional methodology

    NÉSTOR CURVETTO11,2, PABLO MARINANGELI1,2, GABRIELA MOCKEL2

    BIOCELL, Vol.30, No.3, pp. 497-500, 2006, DOI:10.32604/biocell.2006.30.497

    Abstract The micropropagation of Lilium longiflorum requires adequate equipment which may not be afforded by small laboratories or producers. In this work we compared traditional methodology with a protocol that included easily available elements to sterilize materials and culture media, together with addition of hydrogen peroxide (H2O2 ) into the nutrient media as chemical sterilizer. A series of H2O2 concentrations (0.005, 0.010, 0.015 and 0.020% p/v) was used to control contamination during in vitro establishment and subsequent cultivation; the explant organogenic response was also examined and compared to the traditional micropropagation technique. The level of culture contamination was within acceptable limits… More >

  • Open Access

    ARTICLE

    Brief Note : Cytochemical localization of acid phosphatase in Stigeoclonium tenue (Chaetophorales, Chlorophyceae)

    KARINA M. MICHETTI1,2, PATRICIA I. LEONARDI1,3, EDUARDO J. CÁCERES1,4

    BIOCELL, Vol.30, No.3, pp. 491-496, 2006, DOI:10.32604/biocell.2006.30.491

    Abstract Nonspecific acid phosphatases are a group of enzymes whose activity increases the availability of exogenous and endogenous orthophosphate either through extra- or intracellular hydrolysis of phosphate compounds. Our study demonstrates the activity of acid phosphatases in the filamentous freshwater alga Stigeoclonium tenue. These enzymes were detected following a cerium-based method in which cerium was used as an orthophosphate-capture reagent. In thalli from S. tenue from the natural environment, acid phosphatases were found in the longitudinal cell wall, plasmalemma, and vacuole. In thalli from Bold’s Basal Medium culture, these enzymes were found mainly in the plasmalemma; they were scarce in the… More >

  • Open Access

    ARTICLE

    Cysteine proteinases of Trypanosoma cruzi: from digestive enzymes to programmed cell death mediators

    GREGOR KOSEC, VANINA ALVAREZ**¶, JUAN J. CAZZULO**

    BIOCELL, Vol.30, No.3, pp. 479-490, 2006, DOI:10.32604/biocell.2006.30.479

    Abstract Trypanosoma cruzi, the parasite causing Chagas disease, contains a number of proteolytic enzymes. The recent completion of the genome sequence of the T. cruzi CL Brener clone suggests the presence of 70 cysteine peptidases, 40 serine peptidases (none of them from the chymotrypsin family), about 250 metallopeptidases (most leishmanolysin homologues), 25 threonine peptidases, and only two aspartyl peptidases, none of them from the pepsin family. The cysteine peptidases belong to 7 families of Clan CA, 3 families of Clan CD, and one each of Clans CE and CF. In Clan CA, the C1 family is represented by cruzipains 1 and… More >

  • Open Access

    ARTICLE

    MDCK cells express serotonin-regulable 11ß-hydroxysteroid dehydrogenase type 2

    MARISA L. ZALLOCCHI*1, MARIA C. DAMASCO1, JUAN C. CALVO1,2, CARLOS P. LANTOS1, LAURA B. MATKOVIC1

    BIOCELL, Vol.30, No.3, pp. 469-477, 2006, DOI:10.32604/biocell.2006.30.469

    Abstract Prior to this work, we found that adrenal as well as extra-adrenal factors activate the response of renal 11ß-hydroxysteroid dehydrogenase 2 to stressful situations. These results -showing ways through which the organism hinders the pathological occupation of mineralocorticoid receptors by glucocorticoids leading to sodium retention and hypertension- prompted the present study on the nature of the above-mentioned extraadrenal factors. Serotonin was chosen because of its properties as a widely distributed neurohormone, known to interact with glucocorticoids at many sites, also exhibiting increased levels and effects under stressful situations. We studied serotonin effects on 11ß-hydroxysteroid dehydrogenase 2 activity in a cell… More >

  • Open Access

    ARTICLE

    Variation in worm assemblages associated with Pomacea canaliculata (Caenogastropoda, Ampullariidae) in sites near the Río de la Plata estuary, Argentina

    C. DAMBORENEA*, F. BRUSA*, A. PAOLA**

    BIOCELL, Vol.30, No.3, pp. 457-468, 2006, DOI:10.32604/biocell.2006.30.457

    Abstract Pomacea canaliculata is a common gastropod in freshwater habitats from Central and Northern Argentina, extending northwards into the Amazon basin. Several Platyhelminthes have been reported associated to P. canaliculata, sharing an intimate relationship with this gastropod host. The objectives of this study were to describe the symbiotic species assemblages associated to P. canaliculata in the study area, and to disclose differences among them. Samples were taken in three typical small streams and one artificial lentic lagoon, all connected with the Río de la Plata estuary. The 81.53% were infested with different symbiotic (sensu lato) species. Among the Platyhelminthes, the commensal… More >

  • Open Access

    ARTICLE

    Angiotensin II type 1 receptor A1166C GENE polymorphism and essential hypertension in San Luis

    ALICIA VIVIANA LAPIERRE, MARIA ELENA ARCE, JOSÉ RAUL LOPEZ, GLADYS MARÍA CIUFFO.

    BIOCELL, Vol.30, No.3, pp. 447-455, 2006, DOI:10.32604/biocell.2006.30.447

    Abstract Essential hypertension is considered a multifactorial trait resulting from a combination of environmental and genetic factors. The angiotensin II type 1 receptor mediates the vasoconstrictor and growthpromoting effects of Ang II. The A1166C polymorphism of the AT1 receptor gene may be associated with cardiovascular phenotypes, such as high arterial blood pressure, aortic stiffness, and increased cardiovascular risk. We investigated the association between this A1166C polymorphism and hypertension in hypertense and normotense subjects from San Luis (Argentina) by mismatch PCR-RFLP analysis. Hypertense patients exhibited significant increases in lipid related values and body mass index. The frequency of occurrence of the C1166More >

Displaying 15661-15670 on page 1567 of 21909. Per Page