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  • Open Access

    ARTICLE

    Effects of desiccation on Euterpe edulis Martius seeds

    VÍCTOR PANZA1,2, VERÓNICA LÁINEZ2 , SARA MALDONADO1,2, HORACIO L. MARODER3

    BIOCELL, Vol.31, No.3, pp. 383-390, 2007, DOI:10.32604/biocell.2007.31.383

    Abstract Information on desiccation sensitivity of Euterpe edulis seeds under two drying rates is presented. The sensitivity was studied during the course of germination and normal germination. The water content was evaluated for both seeds and embryos. Results showed the following: (a) For both drying treatments and for both germination and normal germination, desiccation sensitivity values were higher for measurements based on the water content of the embryo than for those of the seed. (b) For both drying treatments, desiccation sensitivity were higher for normal germination than for germination based on both the embryo and seed water contents. (c) Under the… More >

  • Open Access

    ARTICLE

    Oxidative stress in Microcystis aeruginosa as a consequence of global climate change

    Marcelo HERNANDO1, Christian HOUGHTON1, Leda GIANNUZZI2, Bernd KROCK3, Darío ANDRINOLO2, Gabriela MALANGA4,*

    BIOCELL, Vol.40, No.1, pp. 23-26, 2016, DOI:10.32604/biocell.2016.40.023

    Abstract Cyanobacteria are phototrophic organisms with great ecological and economical importance. Species of the genus Microcystis are known for their potential ability to synthesize toxins, notably microcystins. There is a growing interest in the evaluation of oxidative stress in relation to the impact of global climate change on natural ecosystems in different trophic levels. Several studies have focused on the analysis of organismal responses to mitigate the damage by controlling the generation of reactive oxygen species. Variations in environmental factors caused by climate change generate a situation of oxidative damage in Microcystis aeruginosa as a direct or indirect consequence. In this… More >

  • Open Access

    ARTICLE

    Estrogen receptors in mast cells from arterial walls

    S. NICOVANI , M.I. RUDOLPH

    BIOCELL, Vol.26, No.1, pp. 15-24, 2002, DOI:10.32604/biocell.2002.26.015

    Abstract We examined the presence of estrogen receptors (ER) in vascular mast cells and a possible genomic effect of estrogens on the expression of mast cell (MC) mediators such as chymase, TNFα, NOS and IL-10, which are known to affect the course of atherosclerosis. Immunocytochemical detection of mast cell tryptase and the co-localization of ERs in MCs from abdominal aortic vessels from 10 fertile woman, 10 postmenopausal women and 15 men was performed. The genomic expression of IL-10, TNFα, and NOS was analyzed by RT-PCR and chymase activity by spectrophotometry after 24 h incubation with 17-β estradiol (0.2-0.5 ng/mL) in rat… More >

  • Open Access

    ARTICLE

    Micropropagation of Prosopis chilensis (Mol.) Stuntz from young and mature plants

    L.A. CARO1, P.A. POLCI1 , L.I. LINDSTRÖM1 , C.V. ECHENIQUE1,2, L.F. HERNÁNDEZ1,3

    BIOCELL, Vol.26, No.1, pp. 25-33, 2002, DOI:10.32604/biocell.2002.26.025

    Abstract Prosopis chilensis (Mol.) Stuntz (Algarrobo de Chile) is an important native tree species that can be grown in arid and semiarid regions for wood and forage production and environmental protection. Developing a simple and reliable in vitro protocol for cloning it would enable to improve it genetically. Explants of P.chilensis were taken from 4 months-old plants grown in the greenhouse or from adult trees grown in a natural environment. Nodal segments 1 – 2 cm long containing an axillary bud were selected from elongating shoots. These cuttings were aseptically cultured on two agar-solid basal media, MS or BTMm, and treated… More >

  • Open Access

    ARTICLE

    Adjuvant effects of Lactobacillus casei added to a renutrition diet in a malnourished mouse model

    PAOLA GAUFFIN CANO1 , GRACIELA AGÜERO3, GABRIELA PERDIGON1,2

    BIOCELL, Vol.26, No.1, pp. 35-48, 2002, DOI:10.32604/biocell.2002.26.035

    Abstract Nutritional deficiencies are associated with impaired immune response, affecting the body’s defence mechanisms. It is also known that Lactic Acid Bacteria (LAB) and fermented products such us yogurt have immunopotentiator activity and nutritional properties, and could thus be used as a valuable supplement in a renutrition diet. The aim of this study was to determine, in a non-severe malnutrition model, the effective dose of Lactobacillus casei (L.casei), which when is used as an adjuvant in a renutrition diet, would modulate the mucosal immune system and induce recovery of the integrity of the intestinal barrier. The experiments were performed on groups… More >

  • Open Access

    ARTICLE

    Prolactin-releasing activity of GHRP-5 (Momany peptide) on lactotrophs in vivo and in vitro

    ANA LUCÍA DE PAUL*, MÓNICA BONATERRA*, CLAUDIA GABRIELA PELLIZAS**, AGUSTÍN AOKI*, ALICIA INÉS TORRES*

    BIOCELL, Vol.26, No.1, pp. 49-60, 2002, DOI:10.32604/biocell.2002.26.049

    Abstract In the present study the in vivo and in vitro effects of GHRP-5 on the PRL-releasing activity in correlation with the morphological changes of lactotroph cells and their transcriptional activity were evaluated. The in vitro treatment (12 µg/100g BW/day for 3 days) of male rats with GHRP-5 does not induce any significant changes in serum PRL levels. In contrast, the addition of GHRP-5 to pituitary cell cultures increased significantly the release of PRL. This effect is enhanced in cell cultures of enriched lactotrophs, increasing significantly the secretion of PRL, the concentrations of which were 50% higher than that of untreated… More >

  • Open Access

    ARTICLE

    Changes of inducible nitric oxide synthase in aortic cells during the development of hypertension: Effect of angiotensin II

    M. Cruzado, C. Castro, N. Risler, R. Miatello

    BIOCELL, Vol.26, No.1, pp. 61-67, 2002, DOI:10.32604/biocell.2002.26.061

    Abstract Nitric oxide (NO) generation by inducible nitric oxide synthase (iNOS) in the vascular smooth muscle cells (VSMC), may play a role in blood vessel tone regulation. Lipopolysaccharide (LPS) induced iNOS activity and subsequent nitrite production by cultured aortic VSMC, from SHR with an established chronic blood pressure elevation (adult SHR) or during the period preceding the development of hypertension (young SHR) and from age-matched normotensive Wistar (W) rats were compared. Angiotensin II (Ang II) effect was also evaluated. Both basal LPS-induced iNOS activity and nitrite accumulation were significantly lower in young SHR VSMC compared to young W rat cells. In… More >

  • Open Access

    ABSTRACT

    Minireview: Lipoproteins from plasma and perivelline fluid of the apple snail Pomacea canaliculata

    H. Heras, R.J. Pollero

    BIOCELL, Vol.26, Suppl.S, pp. 111-118, 2002

    Abstract This article has no abstract. More >

  • Open Access

    ARTICLE

    Time course and mechanism of brain oxidative stress and damage for redox active and inactive transition metals overload

    Nidia FERRAROTTI1, Rosario MUSACCO-SEBIO2, Christian SAPORITO-MAGRIÑÁ2, Juan Manuel ACOSTA2, Marisa REPETTO2 *

    BIOCELL, Vol.40, No.1, pp. 19-22, 2016, DOI:10.32604/biocell.2016.40.019

    Abstract The objective of this work was to study the in vivo time course of biochemical processes of oxidative damage in the brain of Sprague-Dawley rats that received an acute overload of the redox active metals iron (Fe) and copper (Cu), and the redox inactive cobalt (Co) and nickel (Ni). Oxidative stress indicators (phospholipid and protein oxidation), glutathione (GSH), antioxidant enzymes and NADPH oxidase activities, and the plasma inflammatory cytokine (IL-6) were measured. The results showed that in brain oxidative mechanisms for both sets of metal are different, however in both cases are irreversible. The mechanism for Fe and Cu oxidative… More >

  • Open Access

    ARTICLE

    Morphological and histochemical study of the masseter muscle after occlusal alteration

    JOÃO PAULO MARDEGAN ISSA*, RODRIGO TIOSSI*, MAMIE MIZUSAKI IYOMASA**

    BIOCELL, Vol.31, No.3, pp. 375-382, 2007, DOI:10.32604/biocell.2007.31.375

    Abstract The aim of this study was to evaluate alterations in the masseter muscle of 30 male guinea-pigs submitted to occlusal alteration. The animals were divided into 2 equal groups, the control group (C) only submitted to surgical stress, and the occlusal altered group (T) submitted to teeth extraction. Each group was subdivided into 3 groups, with 5 animals, for the following studies: macroscopy and vessels distribution, light microscopy and histochemical analysis, with animals’ perfusion 2 months after surgery. Data were submitted to statistical analysis. Macroscopically, an anteroposterior orientation of the muscular fibers was found and statistical difference between C and… More >

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