García-Zambrano EA¹, MC Ojeda-Zacarías¹, JG Gutiérrez-Soto¹, SR Sinagawa-García¹, A Gutiérrez-Díez¹, J Martínez-de la Cerda¹, JA Pedroza-Flores¹, C Gallegos-Vázquez², JI García-Zambrano³

Phyton-International Journal of Experimental Botany, Vol.87, pp. 87-93, 2018, DOI:10.32604/phyton.2018.87.087

Abstract The technologies based on molecular markers of DNA are the most advanced and possibly the most effective and accurate means to identify genetic variation. The objective of this study was to use molecular genetic markers namely AFLP as a tool for the molecular differentiation of 36 accessions of xoconostle collected in the Centro Regional Universitario Centro Norte of the Universidad Autónoma de Chapingo (CRUCEN-UACh.). DNA extraction was performed by the method of cellular ruptor using FastDNA^® Kit, and quantification of purity and yield of DNA was carried out through the Nanodrop 2000. It was observed that the DNA concentrations ranged… More >

Rodrigues Costa A^1,2, PS Pereira³, MK Alves de Sousa^1,2, FC Rodrigues⁵, VR Duarte Mendes¹, KR Rodrigues Lima¹, L Marivando Barros^1,4, CM Rocha Sousa¹, MS Alcântara¹, AB Leite Cavalcante⁴, R Cruz Macedo⁵, JP Kamdem³, AE Duarte^1,2

Phyton-International Journal of Experimental Botany, Vol.87, pp. 79-86, 2018, DOI:10.32604/phyton.2018.87.079

Abstract The species Rhaphiodon echinus, known as betonica or creeping mint, is considered an infesting plant species, typical of the caatinga biome. Morphoanatomy is a very important tool to study the structure of plants, both its external and internal morphology, opening the knowledge about the production of secondary metabolites. These compounds are of great importance for scientific research, which may present themselves as the best antioxidants. Taking into account a lack of data in the literature on the essential oil of R. echinus, this study aimed to demonstrate its antioxidant activity and toxicity. In order to test this hypothesis, tests were… More >

S.L. MARUÑAK^*, L. LEIVA^**, M.E. GARCIA DENEGRI^*, P. TEIBLER^*, O. ACOSTA DE PÉREZ^*

BIOCELL, Vol.31, No.3, pp. 355-364, 2007, DOI:10.32604/biocell.2007.31.355

Abstract A phospholipase A₂ has been isolated from Bothrops jararacussu venom from snakes that inhabit the northeast region of Argentina. The present study describes in vivo and in vitro biological activities of phospholipase A₂ from B. jararacussu as well as isolation details of its. Venom was obtained by milking of adult snakes which were housing in wood reptile cages of varying dimensions in heated (20–30ºC) rooms. Snakes received a weekly diet of mice and water was available ad libitum for drinking and soaking. The enzyme was purified by gel filtration on a Sephadex G-75 column followed by ion exchange chromatography on… More >

Villa-Hernández JM¹, B García-Ocón², E del C Sierra-Palacios², C Pelayo-Zaldivar³, F Díaz de León-Sánchez¹, LJ Pérez-Flores¹, JA Mendoza-Espinoza²

Phyton-International Journal of Experimental Botany, Vol.87, pp. 72-78, 2018, DOI:10.32604/phyton.2018.87.072

Abstract Mexico bears witness to a long-standing tradition in the use of plants for medicinal purposes; nevertheless, standardized methodological approaches for the proper identification of these are still needed. The problem is especially noticeable during the validation of ingredients in herbal preparations, as so many of them are sold as powders. It is for these reasons that the present study aims to compare the results of classic taxonomical assessments with those obtained using more modern molecular techniques (e.g. PCR-amplified 18S ribosomal RNA gene analysis) in one ethnobotanical case-study carried out in the “Desierto de los Leones” National Park in Mexico City,… More >

Chel-Guerrero LD¹, R Gómez-Cansino², SL Gúzman-Gutierrez³, MG Campos-Lara⁴, E Saury-Duch¹, F Díaz de León Sánchez⁵, R Reyes-Chilpa⁶, JA Mendoza-Espinoza⁷

Phyton-International Journal of Experimental Botany, Vol.87, pp. 68-71, 2018, DOI:10.32604/phyton.2018.87.068

Abstract The objective of this study was to investigate extracts from the peels of Annona squamosa L. (purple sugar apple), Annona reticulata L. (custard apple), Chrysophyllum cainito L. (green star apple), and Melicoccus bijugatus Jacq. (mamoncillo), as potential sources of anti-HIV-1 agents. Methanolic extracts from the peels of the aforementioned tropical fruits were obtained by maceration. Antiviral activity was evaluated through HIV-1 RT inhibition by extracts, using a non-radioactive immuno/colorimetric assay (Lenti RT Activity Assay, Cavidi Tech). Assay was performed according to the protocol provided by the manufacturer. Extracts from the peels of A. squamosa, A. reticulata, and C. cainito showed… More >

FEI XIE^1,2, BAOHONG ZHANG^1,2, WENFENG XU¹, XIAOLING LIAO^1,*, QIUHONG HUANG¹, BO LIU^2,*

BIOCELL, Vol.41, No.2-3, pp. 45-54, 2017, DOI:10.32604/biocell.2017.41.045

Abstract Focal adhesion kinase (FAK) plays a vital role in mediating the adaptability of tumor cells under mechanical stimuli. Previous studies revealed that FAK can locate to different cell compartments, and its regulation is highly dependent on its subcellular localization. However, the local FAK activities and its regulation mechanism in different cell compartments of tumor cells in response to fluid shear stress are still unclear. In this study, 5 dyn/cm² and 20 dyn/cm² of shear stress was applied to HeLa cells for 30 min. The activities of FAK targeting different subcellular compartments (lipids rafts, non-rafts, focal adhesions and cytoplasm) were investigated… More >

A. C. FARALDO¹, A. SÁ-NUNES², L. H. FACCIOLI², E. A. DEL BEL³, E. LELLO⁴

BIOCELL, Vol.31, No.2, pp. 205-211, 2007, DOI:10.32604/biocell.2007.31.205

Abstract Although insects lack the adaptive immune response of the mammalians, they manifest effective innate immune responses, which include both cellular and humoral components. Cellular responses are mediated by hemocytes, and humoral responses include the activation of proteolytic cascades that initiate many events, including NO production. In mammals, nitric oxide synthases (NOSs) are also present in the endothelium, the brain, the adrenal glands, and the platelets. Studies on the distribution of NO-producing systems in invertebrates have revealed functional similarities between NOS in this group and vertebrates. We attempted to localize NOS activity in tissues of naïve (UIL), yeast-injected (YIL), and saline-injected… More >

Gálvez Muñoz YA¹, E Martínez Moreno², S Ramírez Vera², L Latournerie Moreno³, JM Lesher Gordillo¹, G Castañón Nájera^1*

Phyton-International Journal of Experimental Botany, Vol.87, pp. 60-67, 2018, DOI:10.32604/phyton.2018.87.060

Abstract The research was conducted with the aim to identify the variability in situ of wild and semi-wild morphotypes of Capsicum spp. that were found growing in different places of Tabasco and the north of Chiapas States. Morphotypes included “Amashito” (five types), “Pico de paloma” (two types), “Garbanzo”, “Ojo de sapo”, “Ojo de cangrejo”, “Colmillo de lagarto” and “Corazón de pollo”. Such characterization is important because there is an extensive variability of forms cultivated in the country, resulting from a wide range of agroecological diversity as well as diverse forms, colours, flavors and sizes that constitute a valuable collection of genes… More >

BING QI¹, XINMING ZHENG², WENYONG LI³, QINGXIN WEI², QINGXUAN CHEN¹

BIOCELL, Vol.31, No.2, pp. 199-203, 2007, DOI:10.32604/biocell.2007.31.199

Abstract Although there is more evidence that shows that IFNs (interferons) plays a very important role in the early development of the embryo, the mechanism of IFNs is still unclear. Our study showed that IFRG is expressed from oocytes- through to the preimplantation embryo in rabbits. This finding provides some clues for better understanding the role of IFNs in the development of the embryo. The full length of rabbit IFRG cDNA (Accession No. AJ584672), with a 2794bp encoding 131 amino acid sequence, was cloned IFRG expression can be detected in 8 different tissues: ovary, heart, lung, liver, kidney, spleen, cerebra, and… More >

Ithurrart LS^1,2, CA Busso^1,2, YA Torres^1,3, DV Peláez^1,2,3, H Giorgetti⁴, G Rodríguez⁴, ML Ambrosino^2,5

Phyton-International Journal of Experimental Botany, Vol.87, pp. 51-59, 2018, DOI:10.32604/phyton.2018.87.051

Abstract Plant competition for soil resources is common in semiarid rangelands. Plants have various, alternative mechanisms to deal with soil resource acquisition. They include (1) length, weight and proliferation of roots, (2) root length density, and (3) root colonization by arbuscular mycorrhiza. In rangelands of Argentina, plants are exposed to herbivory after natural fires. As a result, knowledge on how these disturbances impact root traits is important for outlining guidelines focused on rangeland management and improvement. Our aim was to analyze the effects of defoliation after a controlled burning on root traits (1) to (3) on two preferred (Poa ligularis and… More >