Yan Li¹, Wei Guo¹, Shen Liu, Bin Zhang, Bing-Bing Yu, Bo Yang, Shun-Li Kan, Shi-Qing Feng

Oncology Research, Vol.25, No.6, pp. 1021-1026, 2017, DOI:10.3727/096504016X14821477992177

Abstract Transmembrane protein 45B (TMEM45B) is a member of the TMEM family of proteins and has been reported to be expressed abnormally in different kinds of human tumors. However, the biological function of TMEM45B in osteosarcoma remains unclear. The objective of this study was to investigate the role of TMEM45B in regulating the biological behavior of osteosarcoma cells. Our results demonstrated that the expression of TMEM45B at both the protein and mRNA levels was dramatically upregulated in human osteosarcoma cell lines. Knockdown of TMEM45B significantly suppressed the proliferation, migration, and invasion of U2OS cells in vitro. More >

Zhenfeng Jiang^*1, Lifen Yao^†1, Hongge Ma^†, Panpan Xu^†, Zhiyan Li^†, Mian Guo^‡, Jianhang Chen^*, Hongbo Bao^§, Shupei Qiao^¶, Yufang Zhao^¶, Jia Shen^#, Minwei Zhu^*, Carolyn Meyers^**, Guizhen Ma^††, Chuncheng Xie^*, Li Liu^*, Haiyang Wang^*, Wang Zhang^*, Qi Dong^†, Hong Shen^*, Zhiguo Lin^*

Oncology Research, Vol.25, No.6, pp. 1009-1019, 2017, DOI:10.3727/096504016X14813859905646

Abstract Pyroptosis is a type of proinflammatory programmed cell death mediated by caspase 1 activity and occurs in several types of eukaryotic tumor cells, including gliomas. MicroRNAs (miRNAs), small endogenous noncoding RNAs, have been demonstrated to be advantageous in glioma therapy. However, the question of whether miRNAs regulate pyroptosis in glioma remains unknown. The current study found that caspase 1 expression was substantially increased in both glioma tissues and glioma cell lines, U87 and T98G, while miR-214 expression was significantly downregulated. Luciferase reporter assay recognized caspase 1 as a target gene of miR-214. These findings demonstrate More >

Nan Zhu¹, Mahui Si¹, Ning Yang, Yingying Jing, Yong Fu, Xijun Zhao, Zhipeng Lin, Guangshun Yang

Oncology Research, Vol.25, No.6, pp. 1001-1008, 2017, DOI:10.3727/096504016X14796039599926

Abstract Ras-association domain family 6 (RASSF6), a member of the RASSF family, is frequently downregulated in various types of cancer. However, the roles of RASSF6 in human hepatocellular carcinoma (HCC) are still unclear. In this study, we investigated the biological functions and related molecular mechanisms in HCC. Our results found that RASSF6 is expressed in low amounts in HCC tissues and cell lines. Overexpression of RASSF6 obviously inhibited the proliferation, invasion, and EMT process in HCC cells. Furthermore, overexpression of RASFF6 greatly downregulated the protein levels of phosphorylated focal adhesion kinase (FAK), MMP-2, and MMP-9 in More >

Yufeng Liu^*, Zhengliang Cheng^†, Feng Pan^‡, Weigang Yan^§

Oncology Research, Vol.25, No.6, pp. 989-999, 2017, DOI:10.3727/096504016X14813867762123

Abstract Spinal osteosarcoma (OS) has been proven to be more difficult to treat owing to potently malignant metastasis. The present study aimed to explore the functional role of microRNA (miR)-373 in cell growth and invasion of OS cells, as well as its underlying mechanism. The expression of miR-373 was analyzed in spinal OS tissues and cell lines. MG-63 cells were transfected with the miR-373 mimic or inhibitor and/or treated with the phosphoinositide 3-kinase (PI3K) (LY294002) inhibitor or Ras-related C3 botulinum toxin substrate 1 (Rac) guanosine triphosphate (GTPase) (NSC23766) inhibitor, and then the impact of miR-373 aberrant… More >

Wenliang Liu^*, Peng Xiao^†, Han Wu^*, Li Wang^*, Demiao Kong^*, Fenglei Yu^*

Oncology Research, Vol.25, No.6, pp. 975-988, 2017, DOI:10.3727/096504016X14791726591124

Abstract MicroRNAs (miRs) have been demonstrated to be significantly associated with the development and progression of non-small cell lung cancer (NSCLC). However, the underlying mechanism of miR-98 in mediating the malignant phenotypes of NSCLC cells remains obscure. In this study, we found that miR-98 was significantly downregulated in NSCLC tissues compared to nontumor lung tissues. Downregulation of miR-98 was significantly associated with poor differentiation and advanced clinical stage. Restoration of miR-98 expression significantly decreased the proliferation, migration, and invasion of NSCLC A549 and H1229 cells. SALL4 was identified as a target gene of miR-98, and the… More >

Fen Liu^*†, Shaojun Liu^*, Feiyan Ai^*†, Decai Zhang^*†, Zhiming Xiao^*, Xinmin Nie^‡, Yunfeng Fu^§

Oncology Research, Vol.25, No.6, pp. 967-974, 2017, DOI:10.3727/096504016X14803476672380

Abstract Colorectal cancer (CRC) is one of the most common malignancies in the world, with a high incidence and a high mortality. However, the pathogenesis of CRC carcinogenesis is still unexplored. In this study, we investigated the role of miR-107 in the regulation of CRC cell proliferation and apoptosis. First, the expression of miR-107 was observed to be aberrantly increased in human CRC tumor tissues and cell lines when compared to the colonic control tissues and colon epithelial cells. Further study showed that the proliferative and apoptotic capacities of human CRC SW480 and LoVo cells were… More >

Jiateng Zhong^*†, Haijun Wang^*, Jian Yu^‡, Jinghang Zhang^‡, Hui Wang^†§

Oncology Research, Vol.25, No.6, pp. 959-965, 2017, DOI:10.3727/096504016X14803482769179

Abstract Forkhead box L1 (FOXL1) is a member of the Forkhead box (FOX) superfamily and was reported to be dysregulated in various types of cancers. However, its expression pattern and underlying cellular function in breast cancer remain largely unexplored. Thus, the aim of this study was to detect FOXL1 expression in breast cancer and to analyze its role in the progression of breast cancer. Our results demonstrated that FOXL1 expression at both the mRNA and protein levels was downregulated in breast cancer tissues and cell lines. Ectopic FOXL1 suppressed breast cancer cell proliferation, migration, and invasion More >

Yu Zhou, Yong Peng, Min Liu, Yugang Jiang

Oncology Research, Vol.25, No.6, pp. 947-957, 2017, DOI:10.3727/096504016X14791732531006

Abstract MicroRNAs (miRs), a class of noncoding RNAs that are 18–25 nucleotides in length, are able to suppress gene expression by targeting complementary regions of mRNAs and inhibiting protein translation. Recently, miR-181b was found to play a suppressive role in glioma, but the regulatory mechanism of miR-181b in the malignant phenotypes of glioma cells remains largely unclear. In this study, we found that miR-181b was significantly downregulated in glioma tissues when compared with normal brain tissues, and decreased miR-181b levels were significantly associated with high-grade pathology and a poor prognosis for patients with glioma. Moreover, miR-181b… More >

Xin Jin^*, Shenghua Tian^†, Pingping Li^‡

Oncology Research, Vol.25, No.6, pp. 939-946, 2017, DOI:10.3727/096504016X14809827856524

Abstract Hepatocellular carcinoma (HCC) is one of the most common malignant diseases in the world. Mutations, overexpression, and improper recruitment of HATs can lead to tumorigenesis. HAT1 is the first histone acetyltransferase identified and is related with developing HCC, but the mechanism is still unclear. Interestingly, we found that HAT1 was upregulated in HCC patient specimens and showed that its upregulation facilitates HCC cell growth in vitro and in vivo. Moreover, we demonstrated that HAT1 promoted glycolysis in HCC cells and knockdown of HAT1 sensitized HCC cells to apoptotic death induced by cisplatin. Our results suggest More >

Jianping Zhou^*, Fan Wang^†, Bingli Liu^‡, Lin Yang^*, Xueying Wang^*, Yu Liu^*

Oncology Research, Vol.25, No.6, pp. 931-937, 2017, DOI:10.3727/096504016X14772424117423

Abstract Pediatric glioma is a devastating brain tumor. Serine threonine tyrosine kinase 1 (STYK1) is a member of the protein tyrosine kinase family and plays a significant role in the formation of several malignant tumors. However, the expression pattern and role of STYK1 in glioma are not yet clear. The aim of this study was to investigate the role and molecular mechanism of STYK1 in glioma. The results showed that STYK1 was highly expressed in glioma cell lines. We also found that knockdown of STYK1 inhibited cell proliferation, migration, and invasion in vitro as well as More >