Yandong Lu^*1, Fangguo Li^*1, Tao Xu^†, Jie Sun^*1

Oncology Research, Vol.23, No.4, pp. 155-163, 2015, DOI:10.3727/096504016X14519157902681

Abstract Chondrosarcoma (CHS) is the second most common malignant bone sarcoma with increased risk of invasion and metastasis. However, the regulatory mechanisms of CHS tumorigenesis remain unknown. Here we investigated the novel role of miR-497 in regulating chondrosarcoma cell growth and cell cycle arrest. RT-PCR analysis showed that the expression of miR-497 is aberrantly downregulated in human chondrosarcoma samples and cells. After transfection with miR-497 mimic or antagomir, the proliferation and apoptosis of JJ012 and OUMS-27 chondrosarcoma cells were determined by CCK-8 assay and flow cytometric analysis, respectively. Results showed that the proliferation capacity of JJ012… More >

Zhong Yu, Xu Zai-Chun, Han Wun-Lun, Zhu Yun-Yun

Oncology Research, Vol.23, No.4, pp. 147-154, 2015, DOI:10.3727/096504016X14519157902645

Abstract Bone morphogenetic protein-7 (BMP-7) inhibited the pathogenesis of renal injury in response to a variety of stimuli. However, little is known about the molecular regulation and mechanism of endogenous BMP-7 and its renoprotective functions. This study examined the regulation of BMP-7 and its role in the fibronectin secretion and apoptosis of NRK-52E cells resulting from transforming growth factor-β1 (TGF-β1) in vitro. Results showed that TGF-β1 promoted factor-associated suicide (FAS), FAS ligand (FASL), fibronectin (FN), and miRNA-21 expression, while it downregulated phospho-Smad1 (pSmad1), pSmad5, and pSmad8 expressions in NRK-52E cells. In contrast, BMP-7 alleviated TGF-β1-induced cell More >

Ming Liu, Dan Wang, Ning Li

Oncology Research, Vol.23, No.5, pp. 257-266, 2015, DOI:10.3727/096504016X14562725373752

Abstract Osteosarcoma (OS) is the most common malignant primary bone tumor disease. HIF-1α was predicted to be the target gene of microRNA-20b (miR-20b). The present study was designed to illustrate the effect of miR- 20b in regulating osteosarcoma via targeting HIF-1α. In this study, we found that the expression of HIF-1α was significantly increased, while miR-20b obviously decreased in OS patients and OS cell lines compared with healthy controls. Moreover, the luciferase report confirmed the targeting reaction between miR-20b and HIF-1α. Additionally, the overexpression of miR-20b suppressed the invasion and growth of both MG63 and U2OS More >

Hongtao Ren^*, Zhongwei Wang^*, Shuqun Zhang^*, Hongbing Ma^*, Yali Wang^*, Lijun Jia^*, Yiming Li^†

Oncology Research, Vol.23, No.5, pp. 249-256, 2015, DOI:10.3727/096504016X14562725373716

Abstract Interleukin-17A (IL-17A) plays a significant role in many inflammatory diseases and cancers. The aim of this study is to investigate the effect of IL-17A on the invasiveness of colorectal cancer. In the study, we found that IL-17A could promote the migration and invasion of colorectal cancer cells. Furthermore, after being treated with IL-17A, the expression and activity of matrix metalloproteinase 2 (MMP-2) and MMP-9 were upregulated. Moreover, the nuclear/overall fractions and DNA-binding activity of p65 and p50 were dramatically elevated by IL-17A. Pretreatment with a nuclear factor-kB (NF-kB) inhibitor (PDTC) or PI3K/AKT inhibitor (LY294002) was More >

Hongwei Zhao, Yubao Zhang, Jianmin Sun, Chao Zhan, Liang Zhao

Oncology Research, Vol.23, No.5, pp. 237-248, 2015, DOI:10.3727/096504016X14562725373671

Abstract Raltitrexed (RTX) is an antimetabolite drug used as a chemotherapeutic agent for treating colorectal cancer, malignant mesothelioma, and gastric cancer. The antitumor capacity of RTX is attributed to its inhibitory activity on thymidylate synthase (TS), a key enzyme in the synthesis of DNA precursors. The current study is aimed at investigating the potential antitumor effects of RTX in liver cancer. Using the HepG2 cell line as an in vitro model of liver cancer, we evaluated the effects of RTX on cell proliferation employing both a WST-8 assay and a clone formation efficiency assay. In addition,… More >

Di Yin^*†1, Yang Li^*1, Baofeng Guo^‡, Zhewen Liu^*, Yang Xu^§, Xiaoqin Wang^*, Yanwei Du^*, Libo Xu^*, Yan Meng^*, Xuejian Zhao^*, Ling Zhang^*

Oncology Research, Vol.23, No.5, pp. 229-236, 2015, DOI:10.3727/096504016X14550280421449

Abstract RNA interference (RNAi) has been used for cancer gene therapy in recent years. However, the application of RNAi is hindered in the absence of safe and efficient gene delivery. In this article, a novel vehicle of graphene oxide functionalized with polyethylenimine and polyethylene glycol (GO-PEI-PEG) was successfully synthetized and then used to deliver plasmid-based Stat3 siRNA. The carrier can readily bind plasmid with high transfection efficiency. Moreover, molecular biology studies reveal that Stat3-related gene and protein expressions were significantly inhibited, suggesting that the formation of GO-PEI-PEG complexes could be utilized as a promising gene delivery More >

Yaohua Fan^*†1, Xiyan Zhu^*1, Qiuping Lan^*, Fang Lou^*, Yu Zheng^*, Haizhou Lou^*, Yong Fang^*, Wei Jin^*, Hongming Pan^*, Kaifeng Wang^*‡

Oncology Research, Vol.23, No.5, pp. 219-228, 2015, DOI:10.3727/096504016X14567549091260

Abstract Radiofrequency ablation (RFA) is a minimally invasive technology for the treatment of liver malignancies and is used as an adjuvant therapy in patients with colorectal liver metastasis (CLM). This study enrolled a total of 49 CLM patients who underwent RFA treatment. Univariate and multivariate analyses were performed using the log-rank test and Cox proportional hazard model, respectively. Univariate analysis showed that OS was closely correlated with tumor size, frequency of RFA treatment, resection of the liver lesion, and CEA levels before RFA (p<0.05). Multivariate analysis revealed that resection of CLM lesions after RFA, frequency of RFA More >

Juan Li, Hui Meng, Yun Bai, Kai Wang

Oncology Research, Vol.23, No.5, pp. 205-217, 2015, DOI:10.3727/096504016X14549667334007

Abstract Metastasis is the primary cause of cancer-related death all over the world. Metastasis is a process by which cancer spreads from the place at which it first arose to distant locations in the body. It is well known that several steps are necessary for this process, including cancer cell epithelial–mesenchymal transition (EMT), cell migration, resistance to anoikis, and angiogenesis. Therefore, investigating the molecular mechanism of regulating cancer metastasis progress may provide helpful insights in the development of efficient diagnosis and therapeutic strategy. Recent studies have indicated that long noncoding RNAs (lncRNAs) play important roles in More >

Huijie Jia^*†1, Tiesuo Zhao^‡§1, Di Zou^¶, Xiaolong Jia^§, Ji Gao^#, Xiangfeng Song^‡§

Oncology Research, Vol.23, No.6, pp. 321-326, 2015, DOI:10.3727/096504016X14570992647041

Abstract Synthetic CpG oligodeoxynucleotides (ODNs), as TLR9 agonists, have been found to play a possible role in antitumor effect. In order to determine the effect of YW002, known as a C-type CpG ODN, on the treatment of hepatocellular carcinoma (HCC), which is one of the most aggressive carcinomas, we chose to inject YW002 at the doses of 12.5 μg and 25 μg per mouse 7 days post-tumor challenge. The survival rate of mice was recorded every day. On day 14 postinjection, five mice in each group were bled and randomly sacrificed. The level of IFN-γ or… More >

Bin Xiao^*1, Chao Liu^*†1, Bing-tong Liu^*1, Xuan Zhang^*, Rong-rong Liu^*, Xue-Wu Zhang^*

Oncology Research, Vol.23, No.6, pp. 311-320, 2015, DOI:10.3727/096504016X14567549091341

Abstract Previous studies have shown that 5,2',4'-trihydroxy-6,7,5'-trimethoxyflavone (TTF1) is the primary anticancer constituent of the traditional Chinese medicinal plant Sorbaria sorbifolia (SS), which has been applied to treat cancer in China. In this study, we investigated the in vitro and in vivo antitumor effects and biological mechanisms of small-molecule TTF1 nanoparticles (TTF1-NPs). The effects of TTF1-NPs on cell growth and apoptosis were investigated using human hepatoma cells. The molecular changes associated with the effects of TTF1-NPs were analyzed by immunocytochemistry and Western blot analysis. The in vivo effect of TTF1-NPs was investigated using the HepG2 tumor xenograft More >