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Journal of Renewable Materials
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DOI: 10.32604/jrm.2022.017848

ARTICLE

The Use of Aloe vera as Natural Coagulant in Algerian Drinking Water Treatment Plant

Abderrezzaq Benalia1,2, Kerroum Derbal2,*, Amel Khalfaoui1, Antonio Pizzi3 and Ghouti Medjahdi4

1Faculty Process Engineering, University of Constantine 3, Constantine, 25000, Algeria
2National Polytechnic School of Constantine, Constantine, 25000, Algeria
3LERMAB-ENSTIB, University of Lorraine, Epinal, 88000, France
4Institut Jean Lamour, Université de Lorraine, CNRS, Nancy, 54011, France
*Corresponding Author: Kerroum Derbal. Email: derbal_kerroum@yahoo.fr
Received: 11 June 2021; Accepted: 29 July 2021

Abstract: The purpose of this work is the study the ability of the plant material Aloe vera to act as natural coagulant using raw water obtained from a drinking water treatment plant (Mila, Algeria). Different solvents such as: NaCl; NaOH and HCl were used as chemical activators to extract the active components from the Aloe vera plant, and different coagulation-flocculation experiments were conducted in a jar test apparatus to evaluate the performance of the extracted coagulant. Also, the effect of coagulant dose on some water parameters such as turbidity, pH, total alkalinity and organic matter were investigated. The results showed that the use of coagulants obtained by using different solvents increases the coagulation efficiency compared to the coagulant obtained from the raw material, for example the maximum turbidity removal efficiency was 28.23, 78.07, 83.46 and 85.15% when using powdered Aloe vera (raw material), AV-NaCl (0.5 M), AV-NaOH (0.05 M) and AV-HCl (0.05 M), respectively. The results defined, that the residual turbidity obtained in this work, where the Aloe vera was used (after treatment by solvents) produced a turbidity lower than the Algerian standard (5 NTU) with initial turbidity (13 NTU). In this study, the infrared spectrum study and analysis has revealed the presence of different functional groups, which are responsible for the coagulation process.

Keywords: Actives components; coagulant; colloidal particles; infrared spectrum; solvent

1  Introduction

The colloidal particles present in natural waters cannot precipitate and form sediments because of their small size. The operation of coagulation-flocculation aims for the growth of particles (which are essentially colloidal) by destabilization of suspended particles and then the formation of flocs by adsorption and aggregation [1]. The destabilization of suspended entities is achieved through adsorption phenomena [2], explained by the double layer theory. It explains how colloids are treated by coagulation [3,4]. The experimental realization of coagulation flocculation aims to determine the optimal concentration of coagulant necessary for a maximum removal of water turbidity [5].

As some studies have shown, coagulants based on aluminum, iron and even synthetic polymers have a significant disadvantage: their proven toxicity to the environment [6] and production of large sludge volumes [7]. Moreover, aluminum is a neurotoxicant and may contribute to Alzheimer’s disease [8]. This has led some researchers to study the possibility of using natural coagulants, biodegradable, non-toxic and available to carry out the coagulation-flocculation process [9].

Most plant-based coagulants cannot feasibly used due to their cost and insufficient worldwide availability, making them difficult to use for water treatment. In addition, year-round availability is an important factor to consider.

The use of natural coagulants has been discouraged in developed countries on the grounds that they have never been subjected to rigorous scientific evaluation [10]. In the developing countries, their development has continued so that today they are beginning to take an interest in this alternative [11]. Traditionally, organic coagulants have been used at the household level. Women in rural Sudan treat their water with Moringa oleifera before using it as drinking water [12].

The natural coagulants like Moringa oleifera, Banana pith, Dillenia indica, Lentil, Chitosan, lime, Cactus have been applied to remove the turbidity [13–15], color [16], heavy metals [17], and improve the chemical oxygen demand (COD) [18,19], remove total suspended solids (TSS) [20], nitrates and sulfates [15,17], alkalinity [15], Conductivity [21], biological oxygen demand (BOD) [22], total phosphor [19], total organic carbon [23], sulfide [24], phosphate (PO43–) [22], Escherichia coli and total coliforms [25–27]. The turbidity removal efficiency is significantly influenced by the active components from natural coagulants. Therefore, these active substances are responsible for coagulation, for instance: proteins [23,28]; polyphenols [29], polysaccharide [30], starches [31,32] and cellulose [33].

Various solvents have been used in the process of extraction of active components to enhance the coagulation, namely distilled water [14,34], KCl [14,35] NaCl [34,35], KNO3 and NaNO3 [36], HCl [34], BaCl2 [37], NaOH [34], NH4Cl [7]. Tab. 1 shows the use of some organic coagulant on turbidity removal using several chemicals.

In a test on the extraction of active coagulating agents, different solvents have to be tested and compared, that’s why we have used three different solvents: a salt (NaCl), a base (NaOH) and an acid (HCl) to improve the coagulation capacity of Aloe vera and thus turbidity removal efficiency. Aloe vera as a natural based coagulant acts differently than the chemical coagulants. The long chains of proteins catch the colloidal pollutants and suspended solids to form flocs that settle down by gravity. Aloe vera can be an alternative coagulant, which can be used for drinking water treatment by natural coagulation process [38].

2  Materials and Methods

2.1 Parameters Evaluation

Raw water and treated water by coagulation-floculation were characterized and the following parameters are defined in the next subdivision: total alkalinity (TA), organic matter were measured according to the standard titrimetric methods [47], pH was obtained by a multi-parameter instrument (Jenway model 3540, Camlab, Cambridge, UK), turbidity measured using HANNA turbidimeter (Code: HI 98713, Hanna instruments, Cluj-Napoca, Romania), which is expressed in nephelometric turbidity units (NTU).

2.2 Raw Water

The initial characterization of the raw water studied (Oued El Athmania drinking water treatment plant, Mila) shows that all parameters accomplish the Algerian drinking water regulation: the pH (7.94), total alkalinity (160 mg CaCO3/L) and organic matter (2.1 mg O2/L), except for turbidity (13 NTU), which exceeded the Algerian standard value (5 NTU) [48].

2.3 Extraction of Active Components from Aloe vera

Aloe vera was collected from a local region near a city located in the north east of Algeria called Mila. The choice of Aloe vera as a natural coagulant in this study was based on its effectiveness, thriftiness and its wide availability. The extraction of active coagulating agents from Aloe vera was carried according to the following steps:

● Drying: the Plant was Dried after Cleaning

The plant was harvested in the Mila region and dried in an oven at 60°C of temperature for 24 h.

● Grinding and Sieving

After drying, a mortar and pestle were used to grind the plant to obtain small diameter seeds. The powder obtained was sieved using a fine sieve to obtain the final powder form with a diameter less than 0.35 mm (Fig. 1).

25 g of the obtained powder was used as bio-coagulant after different treatments such as sodium chloride solution (NaCl) (0.25 M, 0.5 M, 1.0 M); base solution (NaOH) (0.025 M, 0.05 M, 0.1 M); and acid solution (HCl (0.025 M, 0.05 M, 0.1 M), noting that the volume of each solvent was 1,000 mL, after that the resulting solution was stirred for 20 min at 700 rpm (see Fig. 1).

● Filtration

The solution was then allowed to settle for 30 min. After filtration using standard filter (Porosity < 8 μm), the filtrate solution was kept in the cold room at ±4°C and used as coagulant. The different obtained extracts were characterized in terms of proteins [49] and carbohydrates [50] which are summarized in Tab. 2.

Figure 1: Extraction of actives components from Aloe vera

2.4 Experimental Design

A standard jar test apparatus (LI-JTA-125, LABARD, Labard instruments, Bengala, India) was used in the coagulation tests. Samples of the water (500 mL) were stirred at 160 rpm for 3 min and during this time the coagulants (AV-NaCl; AV-NaOH et AV-HCl) were added at different concentrations from 0.1 mL/L to 2.0 mL/L. The stirring speed was then lowered to 30 rpm for 20 min after which the samples were allowed to stand for 30 min.

The turbidity, pH, total alkalinity and organic matter of the supernatant liquors were then measured noting that all values of each parameter were accompanied by an error bar with percentage.

The turbidity was expressed in percentage removal:

TurbidityRemoval(%)=(TI−TR)×100TI (1)

where TI and TR are the initial and residual turbidity, respectively.

3  Results and Discussions

3.1 Characterization of Coagulant

The infrared spectrum of Aloe vera was recorded with a Fourier-Transform Infrared Spectrometer (CHIMATZU Code: HI 98713, Chimatzu, Cluj-Napoca, Romania) (see Fig. 2).

Figure 2: The infrared spectrum of Aloe vera

The active functional groups in Aloe vera are presented in Tab. 3.

The –NH-groups in the amides form intermolecular hydrogen bonds between the coagulant (Aloe vera) and the suspended matter to increase the efficiency of the coagulation process [51].

The presence of CH3 groups most likely indicates the presence of –COOH groups [52]. The carboxyl group provides adsorption sites for suspended solids during the coagulation process [53].

3.2 Effect of Various Solvents as Chemical Activator on Turbidity, pH, Total Alkalinity and Organic Matter

3.2.1 Effect of NaCl

The coagulant dose is the most important parameter in the turbidity removal process, because its effciency depends on the interaction between coagulant agent and cations present in water.

Fig. 3a shows the effect of coagulant dose on turbidity removal efficiency through the treament by NaCl: (AV-NaCl 0.25 M; AV-NaCl 0.5 M; AV-NaCl1 M), it clearly shows that the Aloe vera extract has an effect on turbidity removal.

Among the concentrations tested, 0.5 M NaCl was the most effective one, producing the highest removal of turbidity compared to 0.25 M NaCl and 1M NaCl. The improvement of the coagulation activity for a concentration lower than 0.5 M is due to salting as explained by the Debye-Huckel theory (increasing solvating power) [63], in this case, the ionic salt interacts directly with the active components (proteins) from the coagulant and increases the repulsion between the proteins, which reduces their solubility [7,34,44]. The decrease of the protein solubility in water with an increased salt concentration is known as the salting-out [63]. Salting-out is largely a function of the protein hydrophobicity, the addition of a higher concentrations than 0.5 M NaCl with high hydrophilicity leads to the sequestration of water molecules and will result in a decrease in protein solvation [7,34,44].

In this tests the percentage of turbidity removal was 67.38%, 78.07%, 64.3% when using AV-NaCl 0.25 M; AV-NaCl 0.5 M and AV-NaCl1 M, respectively.

The coagulant extracted from Aloe vera using salt solution NaCl (AV-NaCl 0.25 M; AV-NaCl 0.5 M; AV-NaCl 1M) influenced slightly the pH and total alkalinity of water [34], and can be linked to the nature of treatment considered (Figs. 3b and 3c).

Fig. 3d shows the effect of coagulant dosage on organic matter (AV-NaCl 0.25 M; AV-NaCl 0.5 M; AV-NaCl 1 M). It shows that the addition of coagulant increases the concentration of organic matter in the solution. This increase can be explained by the organic nature of the coagulant, which contains a high content of organic matter, part of which is soluble in water [34,64]. It is worth noting that the high organic matter value were obtained when using 1 M NaCl as solvent, this being around 12 mg O2/L.

Figure 3: Effect of coagulant dosage on: (a) turbidity removal; (b) pH; (c) total alkalinity; (d) organic matter (Effect of NaCl)

3.2.2 Effect of NaOH

Fig. 4a shows turbidity removal when using the Aloe vera coagulant extracted by NaOH at differents concentrations (0.025 M, 0.05 M and 0.1 M). The NaOH-extracted Aloe vera improved the water quality using the bioactive constituents from Aloe vera which gave 77.84%, 83.46% and 69.07% of turbidity removal for the coagulant AV-NaOH 0.025 M; AV-NaOH 0.05 M; AV-NaOH 0.1 M, respectively.

In this study, NaOH concentration of 0.05 M can be considered as the optimum concentration used for the extraction of active components from Aloe vera to improve the coagulation-flocculation process performance [44]. The decrease in turbidity removal for the coagulant AV-0.1 M NaOH is due to denaturation of some active components which are proteins and hence this reduces the Aloe vera coagulant solubility [34,44].

The characteristics of the water (pH, total alkalinity and organic matter) after the Aloe vera treatment with NaOH (AV-NaOH 0.025 M; AV-NaOH 0.05 M; AV-NaOH 0.1 M) are shown in Figs. 4b–4d.

The increase in pH and total alkalinity can be explained by the nature of the solvent used (NaOH) which causes a release of the hydroxide (OH–).

The organic nature of the coagulant used (AV-NaOH) can increase the level of the organic matter in water (Fig. 4d). Whereas, a high organic matter values was obtained for 0.1 M NaOH.

Figure 4: Effect of coagulant dosage on: (a) turbidity removal; (b) pH; (c) total alkalinity; (d) organic matter (Effect of NaOH)

3.2.3 Effect of HCl

Results of turbidity removal using various doses of Aloe vera are shown in Fig. 5a.

In this case, the turbidity removal of water was 68.3%, 85.15% and 59.23% after treatment by coagulant: AV-HCl 0.025 M; AV-HCl 0.05 M; AV-HCl 0.1 M, respectively. In this part, we have obtained a high solubility of active components at 0.05 M [34].

Furthermore, Fig. 5b shows the final pH values of water. The pH value is very important since the coagulation occurs within a specific pH range for the coagulant, the decrease in pH being related to the release of H+.

When using HCl, the decrease in total alkalinity is related to the reaction between H+ ions released by the HCl solvent and HCO3−, OH–, CO32− ions in water [34].

According to Fig. 5d, the final organic matter of water was increased gradually due to the increasing dosage of coagulant. AV-HCl 0.1M yielded the highest reading of organic matter at 12 mg O2/L.

Figure 5: Effect of coagulant dosage on: (a) turbidity removal; (b) pH; (c) total alkalinity; (d) organic matter (Effect of HCl)

4  Conclusion

In the present work, a study has been conducted to investigate the feasibility of natural coagulant for the treatment of drinking water. It was observed that the Aloe vera is able to reduce the initial turbidity.

The residual turbidity measurements indicate that all solvents used have a remarkable effect on the extraction of active components from Aloe vera, which are responsible for coagulation. When using the NaCl, NaOH and HCl solvents; the highest turbidity removal was 78.07%, 83.46%, 85.15%, respectively. Thus, it can be concluded that: (1) AV-NaCl, AV-NaOH and AV-HCl can be used as coagulants, (2) the HCl solvent was the best extracting medium for the active components from Aloe vera when compared to NaCl and NaOH.

The infrared spectrum confirms the presence of various functional groups, which are responsible for the coagulation process.

As perspectives: a comparative study of turbidity removal efficiency of different extraction methods of active coagulating agents from raw vegetable material. As well as the purification of the active components after extraction and used them in place of the raw form. Also a total characterization of raw vegetable material and the extracts obtained from this material.

Acknowledgement: This work was supported by the University Constantine 3.

Funding Statement: The authors received no specific funding for this study.

Conflicts of Interest: The authors declare that they have no conflicts of interest to report regarding the present study.

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