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  • Open Access

    ARTICLE

    Experimental Study of Microalgae Cultivation under Selective Illumination by Ag/CoSO4 for Bioelectrode Materials Preparation

    Kai Zhu1, Hao Chen1,*, Shuang Wang1,*, Chuan Yuan1,2, Bin Cao3, Jun Ni1, Lujiang Xu4, Anqing Zheng5, Arman Amani Babadi1

    Journal of Renewable Materials, Vol.11, No.6, pp. 2849-2864, 2023, DOI:10.32604/jrm.2023.026317

    Abstract Microalgae biomass is an ideal precursor to prepare renewable carbon materials, which has broad application. The bioaccumulation efficiency (lipids, proteins, carbohydrates) and biomass productivity of microalgae are influenced by spectroscopy during the culture process. In this study, a bilayer plate-type photobioreactor was designed to cultivate Chlorella protothecoides with spectral selectivity by nanofluids. Compared to culture without spectral selectivity, the spectral selectivity of Ag/CoSO4 nanofluids increased microalgae biomass by 5.76%, and the spectral selectivity of CoSO4 solution increased by 17.14%. In addition, the spectral selectivity of Ag/CoSO4 nanofluids was more conducive to the accumulation of nutrients (29.46% lipids, 50.66% proteins, and… More > Graphic Abstract

    Experimental Study of Microalgae Cultivation under Selective Illumination by Ag/CoSO<sub>4</sub> for Bioelectrode Materials Preparation

  • Open Access

    ARTICLE

    Transcriptome Analysis and Genes Function Verification of Root Development of Paeonia suffruticosa under Sandy Loam Cultivation

    Yinglong Song1,#, Wenqian Shang1,#, Zheng Wang1,*, Songlin He1,2,*, Xinya Meng3, Liyun Shi1, Yuxiao Shen1, Dan He1, Xueyuan Lou1, Yuke Sun1

    Phyton-International Journal of Experimental Botany, Vol.91, No.12, pp. 2791-2812, 2022, DOI:10.32604/phyton.2022.023572

    Abstract Relatively poor in vitro rooting has limited the large-scale commercial production of tree peony. In this study, on the basis of transcriptome sequencing, differentially expressed genes and the associated metabolic pathways were identified in tree peony roots at different stages of root formation under sandy loam cultivation. A total of 31.63 Gb raw data were generated and 120,188 unigenes (mean length of 911.98 bp) were annotated according to six databases (NR, NT, GO, KEGG, COG, and Swiss-Prot). Analyses of the ungerminated root primordium period, induced root primordium period, and root formation period detected 8,232, 6,907, and 10,687 differentially expressed genes related… More >

  • Open Access

    ARTICLE

    SPAD Value Difference between Blueberry Cultivar ‘STAR’ by Planted Ground and Pot

    Gyung Deok Han1, Dae Ho Jung2, Seong Heo3,*, Yong Suk Chung1,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.11, pp. 2583-2590, 2022, DOI:10.32604/phyton.2022.022866

    Abstract In the smart farm, we can control every detail for production. Collecting every factor that affects the crop’s final yield is necessary to optimize its efficiency. The SPAD values were observed in the ‘Star’ cultivar blueberry (Vaccinium darrowii) three times a day and at three different plant heights. The pattern of SPAD value change was different by the planting position. Ground planted blueberry (V. darrowii) represented a stable SPAD value during the day and at the different heights. However, the SPAD value was increased by time in pot-planted blueberry (V. darrowii). Also, the SPAD value of pot-planted blueberry was lower… More >

  • Open Access

    ARTICLE

    Land Consolidation with Seedling Cultivation Could Decrease Soil Microbial PLFA Diversity

    Shen Zhang1, Yongqi Jian1, Bingjing Yan2, Jin Jin1, Jiasen Wu1, Chenfei Liang1, Juan Liu1,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.8, pp. 1745-1756, 2022, DOI:10.32604/phyton.2022.021076

    Abstract The impact of land consolidation on the soil microbial PLFA diversity is of great importance for understanding the effective arable land usage, improving agricultural ecological conditions and environment. In this study, we collected the soil samples (0–20 cm) in experimental plots with 0 (Z0), 1 (Z1a) and 4 (Z4a) years of land consolidation in the forest station of Ningbo City, Zhejiang Province, southeastern China. The results were analyzed using ANOVA for randomized block design. Compared with control (Z0), the soil pH value under Z1a treatment increased by 14.6%, soil organic carbon (SOC) content decreased by 65.4%, so did the PLFA… More >

  • Open Access

    ARTICLE

    Bioinformatics Analysis of Disease Resistance Gene PR1 and Its Genetic Transformation in Soybeans and Cultivation of Multi-resistant Materials

    Huimin Cui, Shuo Qu, Abraham Lamboro, Yaolei Jiao, Piwu Wang*

    Phyton-International Journal of Experimental Botany, Vol.91, No.7, pp. 1445-1464, 2022, DOI:10.32604/phyton.2022.020010

    Abstract In agricultural production, a single insect-resistant and disease-resistant variety can no longer meet the demand. In this study, the expression vector pCAMBIA-3301-PR1 containing the disease-resistant gene PR1 was constructed by means of genetic engineering, and the PR1 gene was genetically transformed to contain the PR1 gene through the pollen tube method. In CryAb-8Like transgenic high-generation T7 receptor soybean, a new material that is resistant to insects and diseases is obtained. For T2 transformed plants, routine PCR detection, Southern Blot hybridization, fluorescence quantitative PCR detection, indoor and outdoor pest resistance identification and indoor disease resistance identification were performed. The results showed… More >

  • Open Access

    ARTICLE

    Differential Evolution Algorithm Based Self-adaptive Control Strategy for Fed-batch Cultivation of Yeast

    Aiyun Hu1, Sunli Cong1,*, Jian Ding2, Yao Cheng1, Enock Mpofu3

    Computer Systems Science and Engineering, Vol.38, No.1, pp. 65-77, 2021, DOI:10.32604/csse.2021.016404

    Abstract In the fed-batch cultivation of Saccharomyces cerevisiae, excessive glucose addition leads to increased ethanol accumulation, which will reduce the efficiency of glucose utilization and inhibit product synthesis. Insufficient glucose addition limits cell growth. To properly regulate glucose feed, a different evolution algorithm based on self-adaptive control strategy was proposed, consisting of three modules (PID, system identification and parameter optimization). Performance of the proposed and conventional PID controllers was validated and compared in simulated and experimental cultivations. In the simulation, cultivation with the self-adaptive control strategy had a more stable glucose feed rate and concentration, more stable ethanol concentration around the… More >

  • Open Access

    ARTICLE

    Optimization of the in-situ growth conditions based on a novel photo-microcalorimeter for the sustainable cultivation of photosynthetic microorganisms

    YANJUAN LIAO1,#, DONGLING YU2,#, XIAOFANG QIN1, JINMEI WU1, HUI WEI1, HUI LI1, XIANGYING MA1,*, ZAIYIN HUANG1,*

    BIOCELL, Vol.44, No.4, pp. 703-711, 2020, DOI:10.32604/biocell.2020.012047

    Abstract Despite the great potential of photosynthetic microbes in the production of renewable fuels, value-adding chemicals, and water treatment, etc., commercial utilization of them is significantly hindered by the lack of techniques to accurately monitor the thermodynamic and kinetic characteristics of the In-situ growth of microbes under controlled light illumination for optimal cultivation. Herein, we demonstrated that a newly developed highly sensitive photo-microcalorimetric system successfully captured the impacts of the light wavelength and strength on the thermodynamic and kinetic parameters of the In-situ growth of Rhodopseudomonas palustris, a representative photosynthetic microorganism. To our best knowledge, this is the first time that… More >

  • Open Access

    REVIEW

    Salt Stress Threshold in Millets: Perspective on Cultivation on Marginal Lands for Biomass

    Naveed Ul Mushtaq1, Seerat Saleem1, Aadil Rasool1, Wasifa Hafiz Shah1, Khalid Rehman Hakeem2,*, Reiaz Ul Rehman1,*

    Phyton-International Journal of Experimental Botany, Vol.90, No.1, pp. 51-64, 2021, DOI:10.32604/phyton.2020.012163

    Abstract

    Millets hold an immense assurance for food safety and nourishment amid ever-rising agricultural expenses and climate alterations. They are healthful, have supplementary wellbeing profit and need remarkably fewer effort overheads for crop growing. These characters draw attention to millets as a plant of preference for the humankind in the course of emergent alarm about environmental changes. Millets have the prospect to provide biomass and thus bioenergy, reduced carbon emission, carbon footprint and sustainable modern agriculture. As the rate of expansion in budding countries is increasing day by day, the scarcity of energy is a big panic and there is a… More >

  • Open Access

    ARTICLE

    Effects of Biochar Particle Size on Methane Emissions from Rice Cultivation

    Patikorn Sriphirom1,2, Amnat Chidthaisong1,2,3, Kazuyuki Yagi1,2, Nimaradee Boonapatcharoen4, Sudarut Tripetchkul5, Sirintornthep Towprayoon1,2,3,*

    Journal of Renewable Materials, Vol.8, No.10, pp. 1199-1214, 2020, DOI:10.32604/jrm.2020.010826

    Abstract Biochar amendment is generally recognized as an effective mitigation option of methane (CH4) emissions from rice cultivation. Although its mitigation mechanisms are not well understood, the potential relevance of surface area and porosity of biochar has been discussed. This study aimed to evaluate the application of different biochar particle sizes on CH4 production, oxidation, and emissions from rice cultivation in a clay loam soil, based on the assumption that porosity and surface area of biochar are directly related to its mitigation effects. Rice was grown under greenhouse conditions for two growing seasons, either with 0.5–2 mm (small, SB) or with… More >

  • Open Access

    ARTICLE

    Long term diazotrophic cultivation induces phycobiliprotein production in Anabaena variabilis IMU8

    Mohammed Fadhil HADDAD1,2,3, Tugba DAYIOGLU1,2, Barbaros NALBANTOĞLU2, Turgay CAKMAK1,*

    BIOCELL, Vol.43, No.4, pp. 327-333, 2019, DOI:10.32604/biocell.2019.08522

    Abstract Cyanobacteria are considered as a sustainable feedstock for the production of biochemically active compounds such as phycobiliproteins (PBPs). In this study, the impact of nitrogen (N) and phosphorus (P) availability on PBP production of “N-free acclimated” Anabaena variabilis IMU8 was analyzed. Upon isolation and identification, the cyanobacterium has been maintained in N-free BG-11 medium for more than 20 months. For experimentation, the strain was incubated in N-replete, N-depleted, N-P-depleted BG-11 medium. Long-term diazotrophic cultivation of A. variabilis IMU8 resulted in elevated PBP productivity with a limited impact on growth. When compared to N-depleted ones, N supply stimulated a slight induction… More >

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