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Search Results (7)
  • Open Access


    Structure and secretory activity of cultured chondrocytes from patients with osteoarthritis


    BIOCELL, Vol.29, No.2, pp. 163-167, 2005, DOI:10.32604/biocell.2005.29.163

    Abstract Cartilage samples were taken from OA patients in order to describe and quantify pro-inflammatory mediators. Samples were cultured under aseptic conditions in Dulbecco’s modified Eagle medium at 37°C for 10 days. Control samples, taken from non-inflammatory cartilage, were cultured under the same conditions. The levels of NO-2 and NO-3 were measured in the supernatant using a spectrophotometric assay. The activity of MMP-1 was quantified by ELISA.
    The concentration of NO-x was 47.3 ± 4.1 µM in the OA cartilague and 10.7 ± 1.8 µM in the controls. The average MMP-1 activity was 3,650 ± 387 ng/ml in the OA… More >

  • Open Access


    hnRNPK a Possible Mechanosensitive Gene: Its Function in Chondrocytes and Osteoarthritis

    Lucy Wanjiru Njunge1, Andreanne Poppy Estania1, Li Yang1,*

    Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 94-94, 2019, DOI:10.32604/mcb.2019.07116

    Abstract Mechanical stimulation contributes to the development, homeostasis, integrity and functionality of the articular cartilage by modulating several cellular activities including production and remodeling of extracellular matrix (ECM), chondrocyte differentiation, proliferation and apoptosis. On the other hand, abnormal mechanical strain play a critical role in osteoarthritis (OA) pathogenesis by inducing ECM degradation and chondrocyte apoptosis. Furthermore, deleterious mechanical loading can also stimulates the production of pro-inflammatory mediators such as interleukin 1β (IL-1β) that promote to cartilage degradation, chondrocyte hypertrophy and inflammation [1]. Heterogeneous nuclear ribonucleoprotein K (hnRNPK), a member of the hnRNP family, is implicated in the expression, stabilization and organization… More >

  • Open Access


    Chondrocytes and Bone Marrow Staromal Cells Exhibit Differential Responses to Mechanical Stimulation and Cytokine Challenge

    J. M. Taboas1, R. S. Tuan1

    Molecular & Cellular Biomechanics, Vol.3, No.4, pp. 189-191, 2006, DOI:10.32604/mcb.2006.003.189

    Abstract This article has no abstract. More >

  • Open Access


    Hyaluronic acid inhibited the upregulation of heat shock protein 70 in human chondrocytes from osteoarthritis and Kashin-Beck disease

    Zongqiang GAO1, Xiong GUO2, Junchang CHEN1, Chen DUAN2

    BIOCELL, Vol.43, No.2, pp. 99-102, 2019, DOI:10.32604/biocell.2019.06520

    Abstract This study aimed to investigate the effect of hyaluronic acid (HA) on the expression of heat shock protein 70 (HSP70) in chondrocytes isolated from patients with osteoarthritis (OA) and Kashin-Beck disease (KBD). The chondrocytes were collected from OA and KBD patients, and chondrocytes isolated from patients of accident injuries were used as the control. The chondrocytes were treated with HA at different doses. HSP70 expression in chondrocytes at both mRNA and protein levels was tested by PCR and Western blot analysis. Compared with control, both mRNA and protein levels of HSP70 were higher in chondrocytes from KBD and OA. However,… More >

  • Open Access


    Functional Electrospun Poly (Lactic Acid) Scaffolds for Biomedical Applications: Experimental Conditions, Degradation and Biocompatibility Study

    Idalba A. Hidalgo A., Felipe Sojo, Francisco Arvelo, Marcos A. Sabino∗,‡

    Molecular & Cellular Biomechanics, Vol.10, No.2, pp. 85-105, 2013, DOI:10.3970/mcb.2013.010.085

    Abstract The electrospinning technique is a method used to produce nano and microfibers using the influence of electrostatic forces. Porous three dimensional networks of continuous and interconnected fibers as scaffolds were obtained from a poly (lactic acid) solution. The concentration of the polymeric solution, 12.5% m/w, as well as the conditions of voltage (V=11kV) and tip-metallic collector distance (H=13cm) were established to develop these scaffolds through the electrospinning process. The characteristics of the scaffolds, such as fiber diameter, sintering and the biomimetics of the characteristics of a native extra cellular matrix were verified by Scanning Electron Microscopy (SEM). The orientation induced… More >

  • Open Access


    Alteration of Viscoelastic Properties is Associated with a Change in Cytoskeleton Components of Ageing Chondrocytes from Rabbit Knee Articular Cartilage

    Wangping Duan, Lei Wei, Juntao Zhang, Yongzhuang Hao, Chunjiang Li, Hao Li, Qi Li, Quanyou Zhang, Weiyi Chen, Xiaochun Wei∗,§

    Molecular & Cellular Biomechanics, Vol.8, No.4, pp. 253-274, 2011, DOI:10.3970/mcb.2011.008.253

    Abstract The cytoskeleton network is believed to play an important role in the biomechanical properties of the chondrocyte. Ours and other laboratories have demonstrated that chondrocytes exhibit a viscoelastic solid creep behavior in vitro and that viscoelastic properties decrease in osteoarthritic chondrocytes. In this study, we aimed to understand whether the alteration of viscoelastic properties is associated with changes in cytoskeleton components of ageing chondrocytes from rabbit knee articular cartilage. Three age groups were used for this study: young (2-months-old, N=23), adult (8-months-old, N=23), and old (31-months-old, N=23) rabbit groups. Cartilage structure and proteoglycan and type II collagen content were determined… More >

  • Open Access


    Osmotic Loading of in Situ Chondrocytes in Their Native Environment

    Rami K Korhonen∗,†, Sang-Kuy Han, Walter Herzog

    Molecular & Cellular Biomechanics, Vol.7, No.3, pp. 125-134, 2010, DOI:10.3970/mcb.2010.007.125

    Abstract Changes in the osmotic environment cause changes in volume of isolated cells and cells in tissue explants, and the osmotic environment becomes hypotonic in cartilage diseases such as osteoarthritis (OA). However, it is not known how cells respond to a hypotonic osmotic challenge when situated in the fully intact articular cartilage.
    A confocal laser scanning microscope was used to image chondrocytes of intact rabbit patellae in an isotonic (300 mOsm) and hypotonic (172 mOsm) immersion medium. Cell volumes were calculated before and 5, 15, 60, 120 and 240 minutes after the change in saline concentration. Local tissue strains and swelling… More >

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