DULCE MA. BARRADAS-DERMITZ1,*, PATRICIA M. HAYWARD-JONES2, MARTÍN MATA-ROSAS3, BEATRIZ PALMEROSSÁNCHEZ2, OSCAR B. J. PLATAS-BARRADAS4, RODOLFO F. VELÁSQUEZ-TOLEDO2
BIOCELL, Vol.34, No.1, pp. 1-6, 2010, DOI:10.32604/biocell.2010.34.001
Abstract Of the initial six cell lines originating from explants of Taxus globosa, or Mexican yew (stem internode, leaves and meristematic tissue), three were selected for their microbial and oxidation resistance, two from leaves and the other from stem internode. A study of their behavior, both in terms of cell growth, and of baccatin III and paclitaxel production, was developed in suspension cultures with an initially standardized biomass (fresh weight 0.23 g/L) using modified Gamborg’s B5 medium, and an elicitor (methyl jasmonate), on either the first or seventh day of culture, at several levels (0, 0.1, 1, 10, 100 μM). In… More >
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