Vol.44, No.2, 2020, pp.209-215, doi:10.32604/biocell.2020.08941
OPEN ACCESS
ARTICLE
Analysis of isolation of cerebral cortical neurons in rats by different methods
  • JIANHUA LI1,#, YAOGANG ZHANG1,2,#, TAO ZHANG3, MEIYUAN TIAN1, JING HOU1, DENGLIANG HUANG1, YAN CHENG1,3, ZHU MAN1,3, XIAOMING SU1,3, ZHIQIN LI1, SIXIAN TONG1, XUAN ZHANG1,3, JUN DENG1,3, YUN DONG1,3, YANYAN MA1,2,3,*
1 Central Laboratory of Qinghai University Affiliated Hospital, Qinghai University, Xining, 810000, China
2 Qinghai Research Key Laboratory for Echinococcosis, Xining, 810000, China
3 Qinghai University, Xining, 810000, China
* Address correspondence to: Yanyan Ma, xnmayanyan@126.com
# These authors have contributed equally to this work as co-first authors
Received 27 October 2019; Accepted 19 January 2020; Issue published 27 May 2020
Abstract
The aim of this study was to find a way to efficiently separate neuronal cells from the cerebral cortex of adult rats, providing a reference method for rapid acquisition of neuronal cells from the adult rat brain. Fifteen SD rats were randomly divided into three groups, with five SD rats in each group. Then, neuron cells were isolated from the adult rat cerebral cortex by the grinding method, the trypsin method, and the collagenase II method, respectively. The expression of anti-NeuN in the neurons of each group was analyzed by flow cytometry. The acquisition rates and morphology of neurons of each group were observed by immunofluorescence staining. The grinding or collagenase II method is more suitable for rapid acquisition of neuronal cells from an adult rat’s cerebral cortex. The number of neuron cells obtained by the trypsin method were very few, so it is not convenient for later experiments.
Keywords
Neurons, Grinding method, Collagenase II method, Trypsin method, Flow cytometry
Cite This Article
LI, J., ZHANG, Y., ZHANG, T., TIAN, M., HOU, J. et al. (2020). Analysis of isolation of cerebral cortical neurons in rats by different methods. BIOCELL, 44(2), 209–215.
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