Vol.44, No.2, 2020, pp.263-268, doi:10.32604/biocell.2020.09172
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ARTICLE
DNA damage-induced by sodium flouride (NaF) and the effect of cholicalciferol
  • VEYSEL YÜKSEK1,*, SEMIHA DEDE2, AYŞE USTA3, SEDAT ÇETIN2, MEHMET TAŞPINAR4
1 Van Yuzuncu Yil University Özalp Vocational High School, Van, Turkey
2 Department of Biochemistry, Faculty of Veterinary Medicine, VanYuzuncu Yil University, Van, Turkey
3 Department of Chemistry, Faculty of Science, VanYuzuncu Yil University, Van, Turkey
4 Department of Medical Biology, Faculty of Medicine, VanYuzuncu Yil University, Van, Turkey
* Address correspondence to: Veysel Yüksek, veyselyuksek@yyu.edu.tr
Received 18 November 2019; Accepted 15 April 2020; Issue published 27 May 2020
Abstract
It is known that the high electronegativity of fluorine affects various soft tissues, especially the bone structure in organisms. Of these tissues are the kidneys, which play an important role in the excretion of fluoride from the body. Fluoride affects many cellular mechanisms. One of these effects is DNA damage. Our study aimed to investigate the likely protective effect of cholecalciferol (vitamin D3) on genomic DNA damage-induced NaF depending on concentration and time. The IC25 and IC50 values of NaF for 3, 12 and 24 h and optimum dose of increase in proliferation to vitamin D3 through MTT assay in NRK-52E kidney cells were determined. DNA damage was significantly increased (p < 0.05) compared to the control group in all groups except for vitamin D3. It was determined that treatment with NaF together with vitamin D3 decreased the DNA damage compared to NaF treated groups for 3 and 12 h. NaF combined with vitamin D3 was determined statistically to decrease (p < 0.05) DNA damage compared to NaF treated groups for 24 h. As a result, it was determined that the treatment with cytotoxic concentration NaF depending on the time significantly increased (p < 0.05) the genomic DNA damage, but NaF treatment together with vitamin D3 decreased the DNA damage in renal cells depending on the time. It was concluded that vitamin D3 may be useful in preventing DNA damage caused by NaF.
Keywords
NaF, DNA damage, Vitamin D3, Comet assay
Cite This Article
YÜKSEK, V., DEDE, S., USTA, A., ÇETIN, S., TAŞPINAR, M. (2020). DNA damage-induced by sodium flouride (NaF) and the effect of cholicalciferol. BIOCELL, 44(2), 263–268.
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