Vol.88, No.2, 2019, pp.109-117, doi:10.32604/phyton.2019.06734
OPEN ACCESS
ARTICLE
Deterioration of Antioxidant Competence in Barley Lesion Mimic Mutant 194
  • Qunqun Hao1,#, Bo Lyu2,#, Yuhan Tang1,#, Deya Wang1, Yuanyuan Li1, Qingliang Li1, Yuhai Wang1,* and Wenqiang Wang1,*
1 College of Life Sciences, Zaozhuang University, Zaozhuang, 277000, China.
2 State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an, 271018, China.
Abstract
A barley mutant, 194, was observed to exhibit a leaf spot phenotype over the whole course of its growing period. In this study, the phenotype and antioxidant competence were studied in the lesion mimic mutant 194. Plant height was slightly higher in mutant 194 than in the wild type (WT). In addition, leaf spot per plant in mutant 194 was significantly higher than in WT. Antioxidant competence, as indicated by reactive oxygen species (ROS) accumulation, antioxidant enzyme activity, and the expression of antioxidant enzyme-encoding genes was also assessed in mutant 194. Compared to the WT, mutant 194 displayed a relatively higher accumulation of ROS, accompanied by lower activities of some antioxidant enzymes and downregulation of antioxidant enzyme-encoding genes. This demonstrated reduced antioxidant competence in mutant 194. The results suggested that this lower antioxidant competence of mutant 194 could lead to the accumulation of excessive ROS. This excess of ROS could induce programmed cell death and has the potential to promote disease resistance in mutant 194.
Keywords
Lesion mimic mutant; ROS accumulation; antioxidant enzymes; barley
Cite This Article
Hao, Q., Lyu, B., Tang, Y., Wang, D., Li, Y. et al. (2019). Deterioration of Antioxidant Competence in Barley Lesion Mimic Mutant 194. Phyton-International Journal of Experimental Botany, 88(2), 109–117.
This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.