Open Access
ISSN:0031-9457 (print)
ISSN:1851-5657 (online)
Publication Frequency:Monthly
Phyton-International Journal of Experimental Botany is an international journal that publishes research on all aspects of plant science. This journal welcomes original and exciting submissions that provide new and key insights into the origins, growth and development of plants from the molecular to the whole organism and its interactions with the biotic and abiotic environments.
Science Citation Index Expanded (Clarivate Analytics); Journal Citation Report/Science Edition (JCR); Impact Factor (2024): 1.2; 5-Year Impact Factor: 1.3; Biological Abstracts; BIOSIS Previews; Scopus; EMBiology; Latindex; Field Crop Abstracts; CAB Abstracts; CABI Full Text; Periódica; TEEAL, The Essential Electronic Agricultural Library; Núcleo Básico de Revistas Científicas Argentinas (Agosto 2013–Julio 2015); SciELO (Scientific Electronic Library Online); Portal de Revistas en Biodiversidad; Portico, etc...
Open Access
REVIEW
Phyton-International Journal of Experimental Botany, Vol.95, No.4, 2026, DOI:10.32604/phyton.2026.079971 - 28 April 2026
（This article belongs to the Special Issue: Physiological and Ecological Adaptations of Plants to Climate Change)
Abstract Climate change is increasing the frequency and intensity of overlapping abiotic stresses, making cross-tolerance a critical component of plant resilience. While single stress responses have been extensively characterized, plants in natural and agricultural environments frequently encounter simultaneous or sequential stresses such as drought–heat, light–drought, and drought–salinity, which trigger nonadditive and often unpredictable physiological outcomes that vary with stress intensity, timing, and species. This review synthesizes current understanding of the mechanisms underlying cross-tolerance, emphasizing how contradictory signals, stress timing, and physiological integration shape plant responses under combined stress. We highlight how stomatal regulation, leaf energy balance,… More >
Open Access
REVIEW
Phyton-International Journal of Experimental Botany, Vol.95, No.4, 2026, DOI:10.32604/phyton.2026.072898 - 28 April 2026
Abstract Biostimulants, categorized as microbial or non-microbial, including humic substances, seaweed extracts, chitosan, or protein hydrolysates (PHs), have gained significant attention in modern agriculture for their ability to enhance crop productivity, improve nutrient use efficiency, and increase resilience to abiotic and biotic stresses, while reducing dependence on conventional agrochemicals. This review synthesizes the historical development, classification, mechanisms of action, and agronomic benefits of biostimulants, with a particular emphasis on PHs, which are mixtures of amino acids, peptides, and polypeptides derived from plant or animal proteins through enzymatic, chemical, or thermal hydrolysis. The concept of biostimulants has… More >
Open Access
REVIEW
Phyton-International Journal of Experimental Botany, Vol.95, No.4, 2026, DOI:10.32604/phyton.2026.078974 - 28 April 2026
（This article belongs to the Special Issue: Plant Physiological and Molecular Responses to Coupled Water-Nutrient Management: Towards Climate-Resilient Crops)
Abstract Rhizosphere microorganisms, as crucial biological groups at the soil–plant interface, play a significant role in advancing sustainable agriculture. This review systematically synthesizes three decades of research to elucidate the mechanisms and applications of rhizosphere microbes—including nitrogen-fixing bacteria, phosphate-solubilizing microorganisms, and plant growth–promoting rhizobacteria (PGPR)—in enhancing soil health, improving crop stress tolerance, and optimizing ecosystem functioning. Key findings indicate that replacing 50% of synthetic nitrogen with organic fertilizer in maize–wheat rotation systems can reduce nitrous oxide emissions by up to 68% in loamy soils. Long-term no-till systems enhance carbon sequestration through microbial-driven soil organic matter accumulation.… More >
Open Access
ARTICLE
Phyton-International Journal of Experimental Botany, Vol.95, No.4, 2026, DOI:10.32604/phyton.2026.079055 - 28 April 2026
（This article belongs to the Special Issue: Regulators of Plant Growth and Environmental Stress Adaptation)
Abstract This study was conducted to determine the effects of paclobutrazol (PBZ) application methods and dosages on the growth, leaf, flower, and color characteristics of Silene compacta Fisch., a natural species with high potential for use as a seasonal bedding and potted ornamental plant in landscape design. The experiment was carried out under greenhouse conditions, where potted plants received a single PBZ application during the vegetative stage. The study was arranged in a completely randomized design with a 2 × 3 factorial structure. PBZ was applied either as a foliar spray or as a soil drench at… More >
Open Access
ARTICLE
Phyton-International Journal of Experimental Botany, Vol.95, No.4, 2026, DOI:10.32604/phyton.2026.078590 - 28 April 2026
（This article belongs to the Special Issue: Advances in Plant Breeding and Genetic Improvement: Leveraging Molecular Markers and Novel Genetic Strategies)
Abstract Acer truncatum is a significant woody oil-bearing tree species known for its ability to synthesize various unsaturated fatty acids. This study systematically analyzes the lipid metabolic pathways and the associated transcript abundance changes involved in the biosynthesis and accumulation of seed oil in A. truncatum. By integrating lipidomics and transcriptomics analyses across different developmental stages of A. truncatum seeds, we thoroughly investigate the dynamic characteristics of oil metabolism. The results show that triacylglycerols (TAGs) become the dominating lipid class throughout seed development and that their amount increases greatly as the seeds mature, whereas diacylglycerols (DAGs) show a significantly… More >
Open Access
ARTICLE
Phyton-International Journal of Experimental Botany, Vol.95, No.4, 2026, DOI:10.32604/phyton.2026.078378 - 28 April 2026
（This article belongs to the Special Issue: Utilization of Biostimulants in Plant Growth and Health)
Abstract Pepper (Capsicum annuum L.) is highly susceptible to chilling stress, which severely constrains its growth and productivity. Although the eco-friendly biostimulant γ-polyglutamic acid (γ-PGA) has shown promise in enhancing plant tolerance to abiotic stresses, its specific role and underlying mechanisms in alleviating chilling injury in pepper remain poorly understood. This study systematically investigated the physiological and molecular mechanisms by which foliar application of 100 mg·L−1 γ-PGA enhances chilling tolerance in pepper seedlings. Our results demonstrated that γ-PGA pretreatment significantly mitigated chilling-induced growth inhibition and promoted root development, evidenced by a 110.8% increase in the number of root… More >
Open Access
ARTICLE
Phyton-International Journal of Experimental Botany, Vol.95, No.4, 2026, DOI:10.32604/phyton.2026.078088 - 28 April 2026
（This article belongs to the Special Issue: Innovations in Post-Harvest Disease Control and Quality Preservation of Horticultural Crops)
Abstract Citrus fruits are highly susceptible to post-harvest diseases such as green rot (Penicillium digitatum), blue rot (P. italicum), and sour rot (Geotrichum citri-aurantii), causing significant economic losses. Due to the risks associated with synthetic fungicides and the emergence of resistant strains, natural alternatives are needed. This study evaluated the antifungal activity of aqueous extracts of Corrigiola telephiifolia and Marrubium vulgare. In vitro tests were conducted using PDA medium with extract concentrations of 12.5–100 mg/mL and in vivo assays were performed on artificially wounded oranges with the same extract concentrations. In vitro tests showed strong inhibition of mycelial growth and spore germination, with C. telephiifolia More >
Graphic Abstract
Open Access
ARTICLE
Phyton-International Journal of Experimental Botany, Vol.95, No.4, 2026, DOI:10.32604/phyton.2026.076432 - 28 April 2026
（This article belongs to the Special Issue: Technological Advances for Sustainable Management and Biological Control of Plant Pests and Diseases)
Abstract Cucumber mosaic virus (CMV) is among the most widespread plant viruses, infecting over a thousand plant species, including Catharanthus roseus, a medicinal plant valued for producing the anticancer alkaloids vincristine and vinblastine. Despite its economic significance, genomic information on CMV infecting C. roseus in India has been lacking. In this study, we employed small RNA deep sequencing integrated with advanced bioinformatics to generate the first complete genome of CMV infecting C. roseus in India, followed by validation through RT-PCR and Sanger sequencing. The reconstructed tripartite CMV genome encodes replication, silencing suppressor, movement, and coat proteins, consistent with known More >
Graphic Abstract
Open Access
ARTICLE
Phyton-International Journal of Experimental Botany, Vol.95, No.4, 2026, DOI:10.32604/phyton.2026.078884 - 28 April 2026
（This article belongs to the Special Issue: Advances in Ornamental Plants: Micropropagation, Plant Biotechnology, Chromosome Doubling, Mutagenesis, Plant Breeding, Environmental Stress Tolerance, and Postharvest Physiology)
Abstract Artificial polyploidy induction is widely used in ornamental breeding but can yield aneuploidy derivatives that vary in developmental stability and breeding utility. In Hibiscus syriacus ‘Blue Bird’, in vivo colchicine and oryzalin treatments generated regenerated shoots in which genome-size shifts were detected by flow cytometry; among the candidate lines, a subset reached flowering maturity and was characterized in detail. These flowering aneuploids displayed diverse floral alterations, including reduced corolla size, altered pigmentation, and partial conversion of stamens into petaloid organs. Flow cytometry and somatic chromosome counts indicated aneuploid status (150–182 chromosomes). Pollen morphology was highly variable, with… More >
Open Access
ARTICLE
Phyton-International Journal of Experimental Botany, Vol.95, No.4, 2026, DOI:10.32604/phyton.2026.077290 - 28 April 2026
（This article belongs to the Special Issue: Plant Responses to Abiotic Stress)
Abstract The cinnamoyl-CoA reductase (CCR) gene family plays a pivotal role in lignin biosynthesis and plant stress adaptation by catalyzing the first committed step in the monolignol-specific branch of the phenylpropanoid pathway. However, a comprehensive and systematic analysis of CCRs in the economically important Rosa genus remains lacking. Here, we conducted a systematic genome-wide investigation of CCR genes in a diploid species, Rosa chinensis, and a tetraploid cultivar, Rosa × hybrida. We identified 15 and 36 non-redundant CCR genes in R. chinensis and R. × hybrida, respectively. From these, we selected 14 high-confidence orthologs of RcCCR in the R. × hybrida genome as a core… More >
Open Access
ARTICLE
Phyton-International Journal of Experimental Botany, Vol.95, No.4, 2026, DOI:10.32604/phyton.2026.076015 - 28 April 2026
Abstract The fruits of Prunus cerasifera Ehrh. have been traditionally utilized as both medicinal and edible resource, however, their specific phytochemical profile and anti-inflammatory mechanisms remain to be fully elucidated. This study aimed to isolate and identify the chemical constituents from the fruits and evaluate their anti-inflammatory activities. The separation was performed using a combination of chromatographic techniques. The structures of the obtained compounds were elucidated using a combination of 1H and 13C nuclear magnetic resonance (NMR) and electrospray ionization mass spectrometry (ESI-MS). The anti-inflammatory activity of the compounds was initially investigated based on their capacity to inhibit… More >
Open Access
ARTICLE
Phyton-International Journal of Experimental Botany, Vol.95, No.4, 2026, DOI:10.32604/phyton.2026.077713 - 28 April 2026
（This article belongs to the Special Issue: Abiotic Stress in Agricultural Crops)
Abstract Salt stress attenuators assist seed germination by reducing the effects of osmotic and ionic stress, y promoting water uptake, they stabilize enzymes and enhance metabolic tolerance, resulting in higher germination rates and more uniform early development. This study evaluates the effects of applying salt stress attenuators on the germination and seedling formation of O. basilicum as a pre-germination treatment. Two cultivars, Limoncino (tolerant) and Genaro de Menta (sensitive), were subjected to pre-germination treatments combining salt stress with a stress-attenuating agent: no pre-germination treatment and no salt stress; salt stress (6.98 dS m−1 NaCl); hydropriming + salt stress;… More >
Open Access
ARTICLE
Phyton-International Journal of Experimental Botany, Vol.95, No.4, 2026, DOI:10.32604/phyton.2026.078307 - 28 April 2026
（This article belongs to the Special Issue: Advances in Molecular Insights of Plant Secondary Metabolites: Biosynthesis, Regulation, and Applications)
Abstract Pigment biofortification in rice, particularly through enhanced anthocyanin accumulation, is increasingly recognized in Asia for its potential to improve nutritional quality and antioxidant capacity while addressing regional health challenges. However, the regulatory network underlying anthocyanin biosynthesis in rice remains incompletely characterized, especially regarding MYB transcription factors that may control pigment accumulation in diverse germplasm. In this study, a genome-wide analysis of MYB transcription factors in Oryza sativa subsp. japonica identified 109 OsMYB genes. Phylogenetic comparison with known anthocyanin regulators from Arabidopsis thaliana and Oryza rufipogon revealed conserved clades containing potential pigment-related regulators. Integrative analyses including gene structure, conserved motif identification,… More >
Open Access
ARTICLE
Phyton-International Journal of Experimental Botany, Vol.95, No.4, 2026, DOI:10.32604/phyton.2026.078858 - 28 April 2026
Abstract Very long-chain fatty acids (VLCFAs) are widely distributed across plant tissues. 3-Ketoacyl-CoA synthase (KCS) is one of the most crucial enzymes in VLCFA synthesis and markedly influences fatty acid composition in plants. However, the relevant information on KCS proteins in foxtail millet remains poorly understood. In the current study, 30 KCS genes were found in foxtail millet using bioinformatics methods. Phylogenetic data indicated that these genes cluster into eight distinct groups, with members of each group sharing similar motif structures. Further analysis revealed that the cis-acting elements of SiKCS genes are mainly involved in growth and developmental More >
Open Access
ARTICLE
Phyton-International Journal of Experimental Botany, Vol.95, No.4, 2026, DOI:10.32604/phyton.2026.079359 - 28 April 2026
（This article belongs to the Special Issue: Application of Nanomaterials in Plants)
Abstract Soil salinization is an increasingly severe global issue, posing a significant threat to crop growth and food security. Although sorghum exhibits moderate tolerance to saline-alkali stress, it remains highly sensitive to such conditions during the seedling stage. This study investigates the mechanisms by which zinc oxide nanoparticles (ZnO NPs) alleviate saline-alkali stress in sorghum seedlings and determines their optimal application concentration, thereby providing a scientific basis for agricultural production in saline-alkali soils. Hydroponic experiments were conducted to simulate varying degrees of saline-alkali stress. Sorghum seedlings were treated with different concentrations of ZnO NPs (0, 50,… More >
Open Access
ARTICLE
Phyton-International Journal of Experimental Botany, Vol.95, No.4, 2026, DOI:10.32604/phyton.2026.076905 - 28 April 2026
（This article belongs to the Special Issue: Ornamental Plants: Traits, Flowering, Aroma, Molecular Mechanisms, Postharvest Handling, and Application)
Abstract Plant quantitative trait allelic variation stems from complex regulatory networks. Tree peony, which is native to China, is a unique woody plant with ornamental, medicinal, and oil-producing value. Paeonia ostii, as an important species of tree peony, has emerged as a novel woody oil crop in recent years. However, research on functional genes associated with yield traits in P. ostii remains relatively limited. To gain deeper insights into the genetic architecture underlying one of the three key yield components—grain weight—in this study, a genome-wide association map for 123 unrelated P. ostii was constructed by integrating short-read and long-read… More >
Open Access
ARTICLE
Phyton-International Journal of Experimental Botany, Vol.95, No.4, 2026, DOI:10.32604/phyton.2026.077998 - 28 April 2026
（This article belongs to the Special Issue: Microbiome Interactions for Transgenerational Stress Resilience in Crops)
Abstract Staphylococcal meningitis, a severe infection of the meninges, highlights the urgent need for new strategies to combat Staphylococcus aureus (S. aureus) infections. In this study, ethanolic leaf extracts of Coleus forskohlii were evaluated for their antibacterial potential against clinical S. aureus isolates associated with meningitis. Gas chromatography-mass spectrometry (GC-MS) analysis identified 15 phytochemical compounds, two of which—urs-12-en-28-ol (CID 22213452) and petroselaidic acid (CID 5282754) showed promising binding affinities (−7.5 and −5.9 kcal/mol, respectively) against S. aureus protein (30S ribosomal subunit) in molecular docking studies. In vitro assays confirmed the antibacterial activity of the crude extract, with a minimum inhibitory concentration (MIC) More >
Open Access
ARTICLE
Phyton-International Journal of Experimental Botany, Vol.95, No.4, 2026, DOI:10.32604/phyton.2026.078683 - 28 April 2026
Abstract The potting substrate is an important determinant of post-germination growth in Arabidopsis thaliana and Nicotiana tabacum under controlled laboratory conditions. We evaluated four commercially available soil substrates—Sta-Green potting mix plus fertilizer (SPM), Sta-Green flower & vegetable garden soil plus fertilizer (SGS), Miracle-Gro potting mix (MPM), and Miracle-Gro raised bed soil (MBS)—to assess their effects on seed germination and post-germination growth. Germination rates did not differ significantly among substrates for either species. In contrast, post-germination growth was strongly influenced by the substrate, with MPM consistently supporting greater biomass accumulation, stem elongation, and leaf production. Through integrated analysis of More >
Open Access
ARTICLE
Phyton-International Journal of Experimental Botany, Vol.95, No.4, 2026, DOI:10.32604/phyton.2026.078657 - 28 April 2026
（This article belongs to the Special Issue: Advances in the Physiological, Biochemical and Molecular Mechanisms Regulating Fruit Ripening in Tropical Fruits)
Abstract Accurately determining the optimal post-harvest storage period is still a major challenge in mango processing, especially for the Tom EJC (TEJC) variety, due to reliance on subjective visual evaluations, leading to inconsistent product quality and increased post-harvest losses. This study presents an artificial intelligence-based framework combining computer vision and physicochemical analysis to objectively predict the optimal post-harvest storage period of TEJC mango before processing. TEJC mangoes of grade one were stored for eight days at 24–28°C temperature and 66.4–80% relative humidity. Daily measurements of pH, Total Soluble Solids (TSS), firmness, and peel color parameters (L*,… More >
Graphic Abstract
Open Access
ARTICLE
Phyton-International Journal of Experimental Botany, Vol.95, No.4, 2026, DOI:10.32604/phyton.2026.075166 - 28 April 2026
（This article belongs to the Special Issue: Taxonomy, Phytogeography and Ecology of Mediterranean Flora)
Abstract Based on an integrative analysis of morphological traits, phenological characteristics, karyotype, and chloroplast genome data, we revised the taxonomic status of Lycoris haywardii Traub, reclassifying it as a cultivar of Lycoris rosea Traub ex Moldenke, designated as Lycoris rosea ‘Lin’. Lycoris haywardii Traub was originally published by Traub in 1957 as a spring-leafing taxon with orchid purple flowers and stamens slightly shorter than the perianth, with a flowering period from July to mid-August and a purported distribution in Japan. However, populations cultivated in Hangzhou Botanical Garden, previously referred to as L. haywardii, exhibited autumnal leaf emergence, deep purple-pink flowers, and More >
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