Haoxue Wu^#, Xiaohan Zhang^§,#, Xiaoyu Chen, Kang Li, Aixia Xu, Zhen Huang, Jungang Dong, Chengyu Yu^*

Phyton-International Journal of Experimental Botany, Vol.93, No.3, pp. 627-640, 2024, DOI:10.32604/phyton.2024.050321

Abstract Genetic manipulation (either restraint or enhancement) of the biosynthesis pathway of α-linolenic acid (ALA) in seed oil is an important goal in Brassica napus breeding. B. napus is a tetraploid plant whose genome often harbors four and six homologous copies, respectively, of the two fatty acid desaturases FAD2 and FAD3, which control the last two steps of ALA biosynthesis during seed oil accumulation. In this study, we compared their promoters, coding sequences, and expression levels in three high-ALA inbred lines 2006L, R8Q10, and YH25005, a low-ALA line A28, a low-ALA/high-oleic-acid accession SW, and the wildtype ZS11. The expression levels of… More >

Honghui Sun^1,2,#, Ruichao Liu^1,2,#, Yueting Qi¹, Hongsheng Gao¹, Xueting Wang¹, Ning Jiang^1,2, Xiaotong Guo^1,2, Hongxia Zhang¹, Chunyan Yu^1,2,*

Phyton-International Journal of Experimental Botany, Vol.93, No.3, pp. 533-549, 2024, DOI:10.32604/phyton.2024.049417

Abstract Grapes, one of the oldest tree species globally, are rich in vitamins. However, environmental conditions such as low temperature and soil salinization significantly affect grape yield and quality. The glutamate receptor (GLR) family, comprising highly conserved ligand-gated ion channels, regulates plant growth and development in response to stress. In this study, 11 members of the VvGLR gene family in grapes were identified using whole-genome sequence analysis. Bioinformatic methods were employed to analyze the basic physical and chemical properties, phylogenetic trees, conserved domains, motifs, expression patterns, and evolutionary relationships. Phylogenetic and collinear analyses revealed that the VvGLRs were divided into three… More >

Lei Liang, Xiangyang Hu^*

Phyton-International Journal of Experimental Botany, Vol.93, No.3, pp. 517-531, 2024, DOI:10.32604/phyton.2024.049277

Abstract With the rapid development of modern molecular biology and bioinformatics, many studies have proved that transcription factors play an important role in regulating the growth and development of plants. SPATULA (SPT) belongs to the bHLH transcription family and participates in many processes of regulating plant growth and development. This review systemically summarizes the multiple roles of SPT in plant growth, development, and stress response, including seed germination, flowering, leaf size, carpel development, and root elongation, which is helpful for us to better understand the functions of SPT. More >

Dejin Mu^1,2, Lin Chen^1,2, Heze Wang^1,2, Zhaoliu Hu^1,2, Sihui Chen^1,2, Shi Chen^1,2, Nianhui Cai^1,2, Yulan Xu^1,2, Junrong Tang^1,2,*

Phyton-International Journal of Experimental Botany, Vol.93, No.3, pp. 503-516, 2024, DOI:10.32604/phyton.2024.048786

Abstract Phenylalanine ammonia lyase (PAL) is the rate-limiting and pivotal enzyme of the general phenylpropanoid pathway, but few reports have been found on PAL genes in Pinus yunnanensis. In the present study, three PAL genes were cloned and identified from P. yunnanensis seedlings for the first time, namely, PyPAL-1, PyPAL-2, and PyPAL-3. Our results indicated that the open-reading frames of PyPAL genes were 2184, 2157, and 2385 bp. Phylogenetic tree analysis revealed that PyPALs have high homology with other known PAL genes in other plants. In vitro enzymatic analysis showed that all three PyPAL recombinant proteins could catalyze the deamination of… More >

Fenglan Zhao¹, Jizhi Jin¹, Meng Yang¹, Franklin Eduardo Melo Santiago², Jianping Xue¹, Li Xu^3,*, Yongbo Duan^1,*

Phyton-International Journal of Experimental Botany, Vol.93, No.3, pp. 611-625, 2024, DOI:10.32604/phyton.2024.048295

Abstract

Seed germination with selenium (Se) is promising for producing Se-biofortified foods. Mungbean (Vigna radiata (L.) Wilczek) sprout is freshly eaten as a salad dressed with sauce, making it superior for Se biofortification. Since the Se safety range for the human body is extremely narrow, it is imperative to evaluate the genotypic responses of mungbean sprouts to Se. This study evaluated the Se enrichment capacity and interaction with flavonoids and antioxidant systems in sprouts of 20 mungbean germplasms. Selenium treatment was done by immersing mungbean seeds in 20 μM sodium selenite solution for 8 h. Afterward, the biomass, Se amounts, flavonoid… More >

Xueyan Xia^1,#, Xiaohong Fu^2,#, Yu Zhao¹, Jihan Cui¹, Nuoya Xiao¹, Jingxin Wang¹, Yiwei Lu¹, Meihong Huang¹, Cheng Chu¹, Jia Zhang², Mengxin Yang², Shunguo Li^1,*, Jianfeng Liu^2,*

Phyton-International Journal of Experimental Botany, Vol.93, No.3, pp. 445-465, 2024, DOI:10.32604/phyton.2024.048273

Abstract Foxtail millet (Setaria italica) growth was inhibited because of waterlogging stress, which has caused yield reduction. ERF family plays an important role to plant adversity tolerance. In our study, we obtained 19,819 differential expressed genes (DEGs) between the two treatments based on the RNA-seq sequencing of foxtail millet of waterlogging stress. Furthermore, a total of 28 ERF family members were obtained, which have a complete open reading frame. We studied the evolution and function of SiERF family and how they affected the waterlogging tolerance. It was found that SiERF1A/B/C (GenBank ID: OR775217, OR775219, OR775218) and SiRAP2-12 (GenBank ID: OR775216) have… More >

Chun Mao¹, Ji He^1,*, Xuefeng Wen¹, Yangzhou Xiang², Jihong Feng¹, Yingge Shu¹

Phyton-International Journal of Experimental Botany, Vol.93, No.3, pp. 487-502, 2024, DOI:10.32604/phyton.2024.048246

Abstract The contents of carbon (C), nitrogen (N), and phosphorus (P) in soil-microorganisms-plant significantly affect tea quality by altering the main quality components of tea, such as tea polyphenols, amino acids, and caffeine. However, few studies have quantified the effects of these factors on the main quality components of tea. The study aimed to explore the interactions of C, N, and P in soil-microorganisms-plants and the effects of these factors on the main quality components of tea by using the path analysis method. The results indicated that (1) The contents of C, N, and P in soil, microorganisms, and tea plants… More >

Lanying Chen^1,2, Qiumei Quan^3,*, Yunxiang Li³

Phyton-International Journal of Experimental Botany, Vol.93, No.3, pp. 551-566, 2024, DOI:10.32604/phyton.2024.048103

Abstract Mediated by pollen flow, natural hybridization is deemed a crucial factor that propels speciation in floral plants. Despite the fact that the diversity of Epimedium species is concentrated mainly in Southwestern China, the potential impact of interspecific pollination on natural hybridization has yet to be empirically scrutinized. To explore this, we studied the flowering period and the visitors of flowers in three frequently overlapping Epimedium species at Jinchengshan National Forest Park, located in Nanchong, Sichuan Province. Additionally, we performed a series of pollination experiments to examine breeding systems and hybrid compatibility. Morphologically, Epimedium pubescens and Epimedium wushanense are clearly distinct… More >

Kaiyue Hong, Yasmina Radani, Teja Manda, Jinhui Chen, Liming Yang^*

Phyton-International Journal of Experimental Botany, Vol.93, No.3, pp. 413-425, 2024, DOI:10.32604/phyton.2024.047739

Abstract This study explores CAMTA genes in the rare and endangered Chinese plant species, Liriodendron chinense. Despite the completion of whole-genome sequencing, the roles of CAMTA genes in calcium regulation and stress responses in this species remain largely unexplored. Within the L. chinense genome, we identified two CAMTA genes, Lchi09764 and Lchi222536, characterized by four functional domains: CG-1, TIG, ANK repeats, and IQ motifs. Our analyses, including phylogenetic investigations, cis-regulatory element analyses, and chromosomal location studies, aim to elucidate the defining features of CAMTA genes in L. chinense. Applying Weighted Gene Co-Expression Network Analysis (WGCNA), we explored the impact of CAMTA… More >

Xiaojie Liang^1,2, Wei An², Yuekun Li², Yajun Wang², Xiaoya Qin², Yanhong Cui¹, Shuchai Su^1,*

Phyton-International Journal of Experimental Botany, Vol.93, No.3, pp. 467-486, 2024, DOI:10.32604/phyton.2024.047628

Abstract

Goji berry (Lycium barbarum L.) is substantially dependent on nitrogen fertilizer application, which can significantly enhance fruit yield and Goji berry industrial development in Ningxia, China. This study aimed to analyze the functions of differential nitrogen application rates including low (N1), medium (N2), and high (N3) levels in soil microbial community structure (bacterial and fungal) at 2 diverse soil depths (0–20, 20–40 cm) through high-throughput sequencing technology by targeting 16S RNA gene and ITS1 & ITS2 regions. All the observed physicochemical parameters exhibited significant improvement (p < 0.05) with increased levels of nitrogen and the highest values for most parameters… More >