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ARTICLE
Association Analysis of the Growth of Black Poplar (Populus Nigra L.) Under Contrasting Nitrogen Levels
Xihua Liu1, Changjun Ding2, Xiaohua Su2,*
1 Fujian Provincial Key Laboratory of Resources and Environment Monitoring & Sustainable Management and Utilization, Sanming University, Sanming, 36504, China.
2 Research Institute of Forestry, Chinese Academy of Forestry, Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing, 100091, China.
* Corresponding Author: Xiaohua Su. Email: .
Phyton-International Journal of Experimental Botany 2019, 88(4), 425-433. https://doi.org/10.32604/phyton.2019.08285
Abstract
The European black poplar (
Populus nigra L.) has been used as a
germplasm resource for the breeding of new poplar varieties around the world. The
identification and screening of its high nitrogen use efficiency genotypes could
enable the breeding of new resource-efficient poplar varieties. The accessions were
screened using MALDI-TOF MS genotyping technology for ammonium
transporter (
AMT) and nitrate transporters (
NRT) genes against phenotypic data for
seedling height and ground diameter traits, in both low and high nitrogen
environments. Allele re-sequencing of seven genes related to root development
was carried out using the minisequencing method. By cluster analysis, 101
accessions of black poplar were divided into 4 populations, and it was concluded
that Central Europe is the origin of the evolution of low-nitrogen and highefficiency
populations of European black poplar. Association study between SNP
typing and seedling height and ground diameter traits showed that there were
significant correlations between four SNP loci and growth traits under the
contrasting N levels. We found that SNP3 and SNP4 in the
PttAMT1;3 gene were
significantly associated with seedling height traits, and that SNP2 and SNP7 in the
PttAMT1;2 and
PttAMT1;5 genes, respectively, were significantly associated with
ground diameter traits. Thus, considerable allelic diversity is present within the
candidate genes studied and can be utilized to develop functional markers to select
for poplars with improved growth under N stress conditions.
Keywords
Cite This Article
Liu, X., Ding, C., Su, X. (2019). Association Analysis of the Growth of Black Poplar (
Populus Nigra L.) Under Contrasting Nitrogen Levels.
Phyton-International Journal of Experimental Botany, 88(4), 425–433. https://doi.org/10.32604/phyton.2019.08285