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Influence of estradiol analogue on cell growth, morphology and death in esophageal carcinoma cells

THANDI MQOCO1, SUMARI MARAIS1 AND ANNIE JOUBERT
Department of Physiology, University of Pretoria, Pretoria, South Africa
*Address correspondence to: Annie Joubert. E-mail:

BIOCELL 2010, 34(3), 113-120. https://doi.org/10.32604/biocell.2010.34.113

Abstract

2-Methoxyestradiol-bis-sulphamate is a bis-sulphamoylated derivative of the naturally occurring 17-beta-estradiol metabolite namely 2-methoxyestradiol. 2-Methoxyestradiol-bis-sulphamate is regarded as a potential anticancer drug with increased antiproliferative activity when compared to 2-methoxyestradiol. The aim of this pilot in vitro study was to determine the influence of 2-methoxyestradiol-bis-sulphamate on cell growth, morphology and possible induction of certain types of cell death in the SNO esophageal carcinoma cell line. A dose-dependent study (0.2-1.0μM) was conducted with an exposure time of 24 hours. Data revealed that 2-methoxyestradiol-bis-sulphamate reduced cell numbers statistically significantly to 74% after exposure to 0.4μM of the drug. Morphological studies including light microscopy demonstrated hallmarks of apoptosis, while fluorescent microscopy revealed both the presence of apoptosis and autophagy as types of cell death being induced in SNO cells after 24 hours of exposure to 0.4μM 2-methoxyestradiol-bis-sulphamate.

Keywords

cancer, apoptosis, autophagy

Cite This Article

MQOCO, T., MARAIS, S. (2010). Influence of estradiol analogue on cell growth, morphology and death in esophageal carcinoma cells. BIOCELL, 34(3), 113–120.



This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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