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Human umbilical artery smooth muscle exhibits a 2-apb- sensitive capacitative contractile response evoked by vasoactive substances and exprsses mrnas for stim, orai and trpc channelsa

ANA ROCÍO ROLDÁN PALOMO1, PEDRO MARTÍN1, ALEJANDRO REBOLLEDO1*, NICOLÁS ENRIQUE1, LUIS E. FLORES2, VERÓNICA MILESI1

1. GINFIV–Grupo de Investigación en Fisiología Vascular, Facultad de Ciencias Exactas, Universidad Nacional de La Plata. Argentina.
2. CENEXA–Centro de Endocrinología Experimental y Aplicada, (UNLP-CONICET La Plata, Centro Colaborador OPS/ OMS), Facultad de Ciencias Médicas, Universidad Nacional de La Plata. Argentina.

*Address correspondence to: Alejandro Rebolledo. GINFIV, Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata. Calles 47 y 115, La Plata (1900), Argentina. E-mail: email

BIOCELL 2012, 36(2), 73-81. https://doi.org/10.32604/biocell.2012.36.073

Abstract

After depletion of intracellular Ca2+ stores the capacitative response triggers an extracellular Ca2+ influx through store-operated channels (SOCs) which refills these stores. Our objective was to explore if human umbilical artery smooth muscle presented this response and if it was involved in the mechanism of serotonin- and histamine-induced contractions. Intracellular Ca2+ depletion by a Ca2+-free extracellular solution followed by Ca2+ readdition produced a contraction in artery rings which was inhibited by the blocker of Orai and TRPC channels 2-aminoethoxydiphenyl borate (2-APB), suggesting a capacitative response. In presence of 2-APB the magnitude of a second paired contraction by serotonin or histamine was significantly less than a first one, likely because 2-APB inhibited store refilling by capacitative Ca2+ entry. 2-APB inhibition of sarcoplasmic reticulum Ca2+ release was excluded because this blocker did not affect serotonin force development in a Ca2+-free solution. The PCR technique showed the presence of mRNAs for STIM proteins (1 and 2), for Orai proteins (1, 2 and 3) and for TRPC channels (subtypes 1, 3, 4 and 6) in the smooth muscle of the human umbilical artery. Hence, this artery presents a capacitative contractile response triggered by stimulation with physiological vasoconstrictors and expresses mRNAs for proteins and channels previously identified as SOCs.

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APA Style
PALOMO, A.R.R., MARTÍN, P., REBOLLEDO, A., ENRIQUE, N., FLORES, L.E. et al. (2012). Human umbilical artery smooth muscle exhibits a 2-apb- sensitive capacitative contractile response evoked by vasoactive substances and exprsses mrnas for stim, orai and trpc channelsa. BIOCELL, 36(2), 73-81. https://doi.org/10.32604/biocell.2012.36.073
Vancouver Style
PALOMO ARR, MARTÍN P, REBOLLEDO A, ENRIQUE N, FLORES LE, MILESI V. Human umbilical artery smooth muscle exhibits a 2-apb- sensitive capacitative contractile response evoked by vasoactive substances and exprsses mrnas for stim, orai and trpc channelsa. BIOCELL . 2012;36(2):73-81 https://doi.org/10.32604/biocell.2012.36.073
IEEE Style
A.R.R. PALOMO, P. MARTÍN, A. REBOLLEDO, N. ENRIQUE, L.E. FLORES, and V. MILESI "Human umbilical artery smooth muscle exhibits a 2-apb- sensitive capacitative contractile response evoked by vasoactive substances and exprsses mrnas for stim, orai and trpc channelsa," BIOCELL , vol. 36, no. 2, pp. 73-81. 2012. https://doi.org/10.32604/biocell.2012.36.073

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