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Analysis of isolation of cerebral cortical neurons in rats by different methods

JIANHUA LI1,#, YAOGANG ZHANG1,2,#, TAO ZHANG3, MEIYUAN TIAN1, JING HOU1, DENGLIANG HUANG1, YAN CHENG1,3, ZHU MAN1,3, XIAOMING SU1,3, ZHIQIN LI1, SIXIAN TONG1, XUAN ZHANG1,3, JUN DENG1,3, YUN DONG1,3, YANYAN MA1,2,3,*

1 Central Laboratory of Qinghai University Affiliated Hospital, Qinghai University, Xining, 810000, China
2 Qinghai Research Key Laboratory for Echinococcosis, Xining, 810000, China
3 Qinghai University, Xining, 810000, China

* Address correspondence to: Yanyan Ma, email
# These authors have contributed equally to this work as co-first authors

BIOCELL 2020, 44(2), 209-215. https://doi.org/10.32604/biocell.2020.08941

Abstract

The aim of this study was to find a way to efficiently separate neuronal cells from the cerebral cortex of adult rats, providing a reference method for rapid acquisition of neuronal cells from the adult rat brain. Fifteen SD rats were randomly divided into three groups, with five SD rats in each group. Then, neuron cells were isolated from the adult rat cerebral cortex by the grinding method, the trypsin method, and the collagenase II method, respectively. The expression of anti-NeuN in the neurons of each group was analyzed by flow cytometry. The acquisition rates and morphology of neurons of each group were observed by immunofluorescence staining. The grinding or collagenase II method is more suitable for rapid acquisition of neuronal cells from an adult rat’s cerebral cortex. The number of neuron cells obtained by the trypsin method were very few, so it is not convenient for later experiments.

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APA Style
LI, J., ZHANG, Y., ZHANG, T., TIAN, M., HOU, J. et al. (2020). Analysis of isolation of cerebral cortical neurons in rats by different methods. BIOCELL, 44(2), 209-215. https://doi.org/10.32604/biocell.2020.08941
Vancouver Style
LI J, ZHANG Y, ZHANG T, TIAN M, HOU J, HUANG D, et al. Analysis of isolation of cerebral cortical neurons in rats by different methods. BIOCELL . 2020;44(2):209-215 https://doi.org/10.32604/biocell.2020.08941
IEEE Style
J. LI et al., "Analysis of isolation of cerebral cortical neurons in rats by different methods," BIOCELL , vol. 44, no. 2, pp. 209-215. 2020. https://doi.org/10.32604/biocell.2020.08941

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cc This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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