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Leptin promotes proliferation and invasion of osteosarcoma cells by upregulating the expression of SIRT1

HELIN FENG1,2, XIAOCHONG ZHANG3, QIANQIAN ZHANG4, ZE LI5, LILI ZHAO1,6,*

1 Xingtai People’s Hospital Postdoctoral Workstation, Hebei Province Xingtai People’s Hospital, Xingtai, 054031, China
2 Department of Orthopedics, The Fourth Hospital of Hebei Medical University, Shijiazhuang, 050011, China
3 Department of Research and Education, Hebei Province Xingtai People’s Hospital, Xingtai, 054031, China
4 Department of Gynecology, Hebei Medical University Second Affiliated Hospital, Shijiazhuang, 050000, China
5 Department of Emergency, Hebei Medical University Second Affiliated Hospital, Shijiazhuang, 050000, China
6 Department of Orthopedics, Xingtai People’s Hospital, Xingtai, 054031, China

* Address correspondence to: Lili Zhao, email

BIOCELL 2020, 44(3), 443-450. https://doi.org/10.32604/biocell.2020.010705

Abstract

Osteosarcoma (OS) is a primary high-grade malignant bone neoplasm, and the prognosis of OS remains poor due to early metastasis. Leptin plays an essential role in tumorigenesis, but the role of leptin in the development of OS is still not fully understood. In this study, we used a human osteosarcoma MG-63 cell line as an experimental model. MG-63 cells were treated with leptin, and cell proliferation, apoptosis, adhesion, invasion, and gene expression, were evaluated. The results showed that leptin promoted proliferation, decreased adhesion, suppressed apoptosis, and promoted invasion, of MG-63 cells. Moreover, the expression of SIRT1 was upregulated in MG-63 cells exposed to leptin. Furthermore, MMP-2, 8, and 9 were significantly upregulated by SIRT1, while SIRT1 knockdown inhibited the proliferation and invasion of MG-63 cells. In conclusion, our results suggest that leptin promotes OS cell proliferation and invasion by inducing the expression of SIRT1.

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FENG, H., ZHANG, X., ZHANG, Q., LI, Z., ZHAO, L. (2020). Leptin promotes proliferation and invasion of osteosarcoma cells by upregulating the expression of SIRT1. BIOCELL, 44(3), 443–450. https://doi.org/10.32604/biocell.2020.010705

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