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MiR-4262 regulates differentiation and osteogenesis of human periodontal stem cells by targeting suppressor of cytokine signaling 4

ZIMING WEI1,2, YARONG WANG3, MINGYUAN DU1,4, MINJIE LI2, HONG HE1,4,*

1 The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, 430079, China
2 Department of Orthodontics, Stomatological Hospital, Southern Medical University, Guangzhou, 510280, China
3 Department of Periodontology and Implantology, Stomatological Hospital, Southern Medical University, Guangzhou, 510280, China
4 Department of Orthodontics, School and Hospital of Stomatology, Wuhan University, Wuhan, 430079, China

* Address correspondence to: Hong He, email

BIOCELL 2020, 44(4), 631-638. https://doi.org/10.32604/biocell.2020.010596

Abstract

Periodontitis, as a chronic inflammatory disease, remains unsolved, and the pathogenesis of this disease has not been fully elucidated. In this study, the effect of miR-4262 was investigated in tumor necrosis factor-α (TNF-α) induced human periodontal stem cells (hPDLSCs) for the first time. The gene expression involved in this study was determined using polymerase chain reaction (PCR), the expressions of relevant proteins were determined by western blot analysis, and the levels of IL-1β, IL-6, and MCP-1 were estimated by enzyme-linked immunosorbent assay (ELISA) assay. The luciferase reporter assay was performed for verification of the target gene, alkaline phosphatase (ALP) activity detection was used for differentiation capacity, and alizarin red staining assay was used for mineralization capacity. The inhibition of miRNA-4262, which resulted in the upregulation of suppressor of cytokine signaling 4 (SOCS4), showed protective effects, including anti-inflammation and promotional effects on osteogenesis as well as differentiation in TNF-α induced hPDLSCs. These results provided insights into the roles of miRNAs in regulating the inflammatory response, differentiation, and osteogenesis in hPDLSCs, which may promote the understanding of the mechanisms of periodontitis and find out a better therapeutic application.

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WEI, Z., WANG, Y., DU, M., LI, M., HE, H. (2020). MiR-4262 regulates differentiation and osteogenesis of human periodontal stem cells by targeting suppressor of cytokine signaling 4. BIOCELL, 44(4), 631–638.

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