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Chaperone-mediated autophagy targeting chimeras (CMATAC) for the degradation of ERα in breast cancer

JUN ZHANG, YEHONG HUANG, WENZHUO LIU, LULU LI, LIMING CHEN*

Jiangsu Key Laboratory for Molecular and Medical Biotechnology, Department of Biochemistry, School of Life Sciences, Nanjing Normal University, Nanjing, 210023, China

* Address correspondence to: Liming Chen, email

BIOCELL 2020, 44(4), 591-595. https://doi.org/10.32604/biocell.2020.011642

Abstract

Estrogen receptor alpha (ERα/ESR1) is overexpressed in over half of all breast cancers and is considered a valuable therapeutic target in ERα positive breast cancer. Here, we designed a membrane-permeant Chaperonemediated Autophagy Targeting Chimeras (CMATAC) peptide to knockdown endogenous ERα protein through chaperone-mediated autophagy. The peptide contains a cell membrane-penetrating peptide (TAT) that allows the peptide to by-pass the plasma membrane, an αI peptide as a protein-binding peptide (PBD) that binds specifically to ERα, and CMA-targeting peptide (CTM) that targeting chaperone-mediated autophagy. We validated that ERα targeting peptide was able to target and degrade ERα to reduce the viability of ERα positive breast cancer cells. Taken together, our studies provided a new method to reduce the level of intracellular ERα protein via CMATAC, and thus may provide a new strategy for the treatment of ERα positive breast cancer.

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APA Style
ZHANG, J., HUANG, Y., LIU, W., LI, L., CHEN, L. (2020). Chaperone-mediated autophagy targeting chimeras (CMATAC) for the degradation of ERα in breast cancer. BIOCELL, 44(4), 591-595. https://doi.org/10.32604/biocell.2020.011642
Vancouver Style
ZHANG J, HUANG Y, LIU W, LI L, CHEN L. Chaperone-mediated autophagy targeting chimeras (CMATAC) for the degradation of ERα in breast cancer. BIOCELL . 2020;44(4):591-595 https://doi.org/10.32604/biocell.2020.011642
IEEE Style
J. ZHANG, Y. HUANG, W. LIU, L. LI, and L. CHEN "Chaperone-mediated autophagy targeting chimeras (CMATAC) for the degradation of ERα in breast cancer," BIOCELL , vol. 44, no. 4, pp. 591-595. 2020. https://doi.org/10.32604/biocell.2020.011642

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cc This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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