GP30 of the mycobacteriophage CASbig impairs mycobacterial adaptation during acidic stress and in macrophages
- WU LI1, JIANGZHE SI2, SHUAI QIU2, JING LUO1, HUIQIONG SUN1, XIAOQING LI1, ZHIBIN WAN1, WEI GAO1, HANLU ZOU1, LEI ZHANG2, XIAOHONG XIANG3, YANZHANG LI2, TIESHAN TENG2,*
1 Key Laboratory of Regional Characteristic Agricultural Resources, College of Life Sciences, Neijiang Normal University, Neijiang, 641100, China
2 School of Medical Sciences, College of Medicine, Henan University, Kaifeng, 475004, China
3 School of Pharmacy, Chongqing Medical and Pharmaceutical College, Chongqing, 401331, China
* Address correspondence to: Tieshan Teng,
(This article belongs to this Special Issue: Bacteriophage Biology and Biotechnology)
2 School of Medical Sciences, College of Medicine, Henan University, Kaifeng, 475004, China
3 School of Pharmacy, Chongqing Medical and Pharmaceutical College, Chongqing, 401331, China
* Address correspondence to: Tieshan Teng,
(This article belongs to this Special Issue: Bacteriophage Biology and Biotechnology)
Received 06 June 2020; Accepted 12 August 2020; Issue published 24 December 2020
Abstract
The rapid emergence of multidrug-resistant and extensively drug-resistant Tuberculosis retrieved intense
interest in phage-based therapy. This old approach, which was abandoned in the west in the 1940s but is generating
renewed interest, has stimulated fresh research on mycobacteriophages and their lytic efficiency against their hosts.
GP30 is a novel protein of the mycobacteriophage CASbig with undiscovered function. In this study, we analyzed the
role of CASbig gp30 in the host Mycobacterium smegmatis. Overexpression of gp30 in the host led to reduced growth
in acidic medium and attenuated the intracellular survival rate of M. smegmatis inside the THP-1 macrophages,
which may be linked to the altered lipid profile of the recombinant bacterial cell wall. In a word, this study suggested
that gp30, a novel gene from a mycobacteriophage, modulated lipid composition and content to hamper the
survivability of bacteria under stress conditions.
Keywords
Mycobacterium smegmatis, gp30, CASbig, Acidic stress, Macrophage
Cite This Article
LI, W., SI, J., QIU, S., LUO, J., SUN, H. et al. (2020). GP30 of the mycobacteriophage CASbig impairs mycobacterial adaptation during acidic stress and in macrophages. BIOCELL, 44(4), 695–701.
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