Open Access
ARTICLE
MBD2 promotes Th2 differentiation in ovalbumin-induced CD4+ T cells
QILU PAN1,2,#, YAN JIANG1,2,#, LINQIAO LI1,2, XIAOJING DU1, QIAN HAN1, FEIXIANG LING1, ROU LI1, SHUYUAN CHU1,2, LIN MAI1, JIANWEI HUANG1, LIBING MA1,2,*
1 Department of Respiratory and Critical Care Medicine, Affiliated Hospital of Guilin Medical University, Guilin, 541001, China
2 Laboratory of Respiratory Disease, Affiliated Hospital of Guilin Medical University, Guilin, 541001, China
* Corresponding Author: LIBING MA. Email:
BIOCELL 2023, 47(11), 2495-2502. https://doi.org/10.32604/biocell.2023.042617
Received 06 June 2023; Accepted 20 September 2023; Issue published 27 November 2023
Abstract
Introduction: Allergen-specific CD4
+ T cells play a central role in autoimmune disorders, allergies and asthma, with Th2-type immunity being the typical functional response of CD4
+ T cells. This study aimed to investigate the role of MBD2 in regulating Th2 cell differentiation.
Methods: Splenic mononuclear cells were extracted from C57BL/6 mice, and CD4
+ T cells were isolated using magnetic beads and confirmed through flow cytometry. Lentivirus was employed to construct MBD2-silenced CD4
+ T cells.
In vitro experiments were performed to treat splenogenic mononuclear cells and CD4
+ T cells with Ovalbumin (OVA), and Th2 cell ratios and IL-4 levels were assessed using flow cytometry and ELISA.
Results: The purity of the isolated CD4
+ T cells was 95.73%, confirming successful isolation of primary CD4
+ T cells. Compared to the control group, the Th2 cell ratio exhibited an increase in the Th2-induced group. Treatment with 5-Aza (concentrations, 1–100 μM) promoted Th2 cell differentiation and increased IL-4 levels. Notably, when combined with Th2 induction and 10 μM 5-Aza treatment, silencing MBD2 further amplified Th2 cell ratios and elevated IL-4 levels in cell supernatants. Furthermore, OVA (concentration, 200 μg/mL) induced the differentiation of CD4
+ T cells into Th2 cells and increased IL-4 secretion. Interestingly, silencing MBD2 significantly increased the Th2 cell ratio and IL-4 levels in OVA-treated CD4
+ T cells.
Conclusion: In summary, OVA promoted CD4
+ T cell differentiation into Th2 cells and enhanced IL-4 levels. MBD2 was identified as a mediator of Th2 cell differentiation in splenic-derived CD4
+ T cells, influenced by OVA or 5-Aza treatment.
Keywords
Cite This Article
APA Style
PAN, Q., JIANG, Y., LI, L., DU, X., HAN, Q. et al. (2023). MBD2 promotes th2 differentiation in ovalbumin-induced cd4<sup>+</sup> T cells. BIOCELL, 47(11), 2495-2502. https://doi.org/10.32604/biocell.2023.042617
Vancouver Style
PAN Q, JIANG Y, LI L, DU X, HAN Q, LING F, et al. MBD2 promotes th2 differentiation in ovalbumin-induced cd4<sup>+</sup> T cells. BIOCELL . 2023;47(11):2495-2502 https://doi.org/10.32604/biocell.2023.042617
IEEE Style
Q. PAN et al., "MBD2 promotes Th2 differentiation in ovalbumin-induced CD4<sup>+</sup> T cells," BIOCELL , vol. 47, no. 11, pp. 2495-2502. 2023. https://doi.org/10.32604/biocell.2023.042617