Open Access
ARTICLE
Anti-proliferative effect of Annona extracts on breast cancer cells
MARIA-LUISA VEISAGA1,2, MARIAM AHUMADA2, STACY SORIANO2, LEONARDO ACUNA3, WEI ZHANG3, IVY LEUNG2, ROBERT BARNUM2, MANUEL A. BARBIERI1,2,3,4,5,*
1 Biomolecular Sciences Institute, Florida International University, Miami, 33199, USA
2 Department of Biological Sciences, Florida International University, Miami, 33199, USA
3 Biochemistry Ph.D. Program, Florida International University, Miami, 33199, USA
4 Fairchild Tropical Botanic Garden, Coral Gables, 33156, USA
5 International Center of Tropical Botany, Florida International University, Miami, 33199, USA
* Corresponding Author: Manuel A. Barbieri,
(This article belongs to the Special Issue: Natural Products for Chronic Inflammatory Diseases: Pharmacology and Toxicology)
BIOCELL 2023, 47(8), 1835-1852. https://doi.org/10.32604/biocell.2023.029076
Received 31 January 2023; Accepted 01 June 2023; Issue published 28 August 2023
Abstract
Backgorund: Fruits and seed extracts of
Annona montana have significant cytotoxic potential in several cancer
cells. This study evaluates the effect of
A. montana leaves hexane extract on several signaling cascades and gene expression
in metastatic breast cancer cells upon insulin-like growth factor-1 (IGF-1) stimulation.
Methods: MTT assay was
performed to determine the proliferation of cancer cells. Propidium iodide staining and flow cytometry analysis of
Annexin V binding was utilized to measure the progression of the cell cycle and the induction of apoptosis. Protein
expression and phosphorylation were determined by western blotting analysis to examine the underlying cellular
mechanism triggered upon treatment with
A. montana leaves hexane extract.
Results: A. montana leaves hexane (subfraction V) blocked the constitutive stimulation of the PI3K/mTOR signaling pathways. This inhibitory effect was
associated with apoptosis induction as evidenced by the positivity with Annexin V and terminal deoxynucleotidyl
transferase dUTP nick end labeling (TUNNEL) staining, activation of caspase-3, and cleavage of PPAR. It also limited
the expression of various downstream genes that regulate proliferation, survival, metastasis, and angiogenesis (i.e.,
cyclin D1, survivin, COX-2, and VEGF). It increased the expression of p53 and p21. Interestingly, we also observed
that this extract blocked the activation of AKT and ERK without affecting the phosphorylation of the IGF-1 receptor
and activation of Ras upon IGF-1 stimulation.
Conclusion: Our study indicates that
A. montana leaves (sub-fraction
V) extract exhibits a selective anti-proliferative and proapoptotic effect on the metastatic MDA-MB-231 breast cancer
cells through the involvement of PI3K/AKT/mTOR/S6K1 pathways.
Keywords
Cite This Article
APA Style
VEISAGA, M., AHUMADA, M., SORIANO, S., ACUNA, L., ZHANG, W. et al. (2023). Anti-proliferative effect of <i>annona</i> extracts on breast cancer cells. BIOCELL, 47(8), 1835-1852. https://doi.org/10.32604/biocell.2023.029076
Vancouver Style
VEISAGA M, AHUMADA M, SORIANO S, ACUNA L, ZHANG W, LEUNG I, et al. Anti-proliferative effect of <i>annona</i> extracts on breast cancer cells. BIOCELL . 2023;47(8):1835-1852 https://doi.org/10.32604/biocell.2023.029076
IEEE Style
M. VEISAGA et al., "Anti-proliferative effect of <i>Annona</i> extracts on breast cancer cells," BIOCELL , vol. 47, no. 8, pp. 1835-1852. 2023. https://doi.org/10.32604/biocell.2023.029076