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Differential Orientation of 10T1/2 Mesenchymal Cells on Non-Uniform Stretch Environments

WJ Richardson, DD van der Voort, E Wilson, JE Moore Jr.∗,‡

Department of Biomedical Engineering, Texas A&M University, 5045 Emerging Technologies Building, 3120 TAMU, College Station, TX 77843, USA. Tel: 979-845-5532. Fax: 979-845-4450.
Department of Medical Physiology, Texas A&M Health Science Center, 336 Reynolds Medical Building, College Station, TX 77843, USA. Tel: 979-845-7816. Fax: 979-862-4638.
Corresponding Author: The Bagrit and Royal Academy of Engineering Chair in Medical Device Design. Royal Society-Wolfson Research Merit Award Holder. Department of Bioengineering, Imperial College London, South Kensington Campus, Royal School of Mines Building, Room 4.14, London, SW7 2AZ, United Kingdom. Tel: +44 (0)20 759 49795. Fax: +44 (0)20 7594 9817. Email: james.moore.jr@imperial.ac.uk

Molecular & Cellular Biomechanics 2013, 10(3), 245-265. https://doi.org/10.3970/mcb.2013.010.245


Non-uniform stress and strain fields are prevalent in many tissues in vivo, and often exacerbated by disease or injury. These mechanical gradients potentially play a role in contributing to pathological conditions, presenting a need for experimental tools to allow investigation of cell behavior within non-uniformly stimulated environments. Herein, we employ two in vitro cell-stretching devices (one previously published; one newly presented) capable of subjecting cells to cyclic, non-uniform stretches upon the surface of either a circular elastomeric membrane or a cylindrical PDMS tube. After 24 hours of cyclic stretch, 10T1/2 cells on both devices showed marked changes in long-axis orientation, with tendencies to align parallel to the direction of minimal deformation. The degree of this response varied depending on location within the stretch gradients. These results demonstrated the feasibility of conducting cell mechanobiology investigations with the two novel devices, while also highlighting the experimental capabilities of non-uniform mechanical environments for these types of studies. Such capabilities include robust data collection for developing mechanobiological dose-response curves, signal threshold identification, and potential spatial targeting for drug delivery.

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APA Style
Richardson, W., Voort, D.V.D., Wilson, E., Jr., J.M. (2013). Differential orientation of 10T1/2 mesenchymal cells on non-uniform stretch environments. Molecular & Cellular Biomechanics, 10(3), 245-265. https://doi.org/10.3970/mcb.2013.010.245
Vancouver Style
Richardson W, Voort DVD, Wilson E, Jr. JM. Differential orientation of 10T1/2 mesenchymal cells on non-uniform stretch environments. Mol Cellular Biomechanics . 2013;10(3):245-265 https://doi.org/10.3970/mcb.2013.010.245
IEEE Style
W. Richardson, D.V.D. Voort, E. Wilson, and J.M. Jr. "Differential Orientation of 10T1/2 Mesenchymal Cells on Non-Uniform Stretch Environments," Mol. Cellular Biomechanics , vol. 10, no. 3, pp. 245-265. 2013. https://doi.org/10.3970/mcb.2013.010.245

cc This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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