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Mechanical Stretch-Induced Changes in Cell Morphology and mRNA Expression of Tendon/Ligament-Associated Genes in Rat Bone-Marrow Mesenchymal Stem Cells

Guanbin Song∗,†,‡, Qing Luo*, Baiyao Xu*, Yang Ju

Key Laboratory of Biorheological Science and Technology, Ministry of Education of the People’s Republic of China. College of Bioengineering, Chongqing University, Chongqing 400044, People’s Republic of China.
Department of Mechanical Science & Engineering, Graduate School of Engineering, Nagoya University, Nagoya 464-8603, Japan.
Corresponding author. Tel.: +86 23 6510 2507, Fax: +86 23 6510 6062, E-mail: song@cqu.edu.cn

Molecular & Cellular Biomechanics 2010, 7(3), 165-174. https://doi.org/10.3970/mcb.2010.007.165

Abstract

It has been demonstrated that mechanical stimulation plays a vital role in regulating the proliferation and differentiation of stem cells. However, little is known about the effects of mechanical stress on tendon/ligament development from mesenchymal stem cells (MSCs). Here, using a custom-made cell-stretching device, we studied the effects of mechanical stretching on the cell morphology and mRNA expression of several key genes modulating tendon/ligament genesis. We demonstrate that bone-marrow-derived rat MSCs (rMSCs), when subjected to cyclic uniaxial stretching, express obvious detectable mRNAs for tenascin C and scleraxis, a unique maker of tendon/ligament formation, and significantly increased levels of type I collagen and type III collagen mRNAs. The stretched cells also orient at approximately~65 with respect to the stretching direction and exhibit a more fibroblast-like morphology. Collectively, these results indicate that mechanical stretching facilitates the directed differentiation of rMSCs into tendon/ligament fibroblasts, which has potential implications for the tissue engineering of bioartificial tendons and ligaments.

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APA Style
Song, G., Luo, Q., Xu, B., Ju, Y. (2010). Mechanical stretch-induced changes in cell morphology and mrna expression of tendon/ligament-associated genes in rat bone-marrow mesenchymal stem cells. Molecular & Cellular Biomechanics, 7(3), 165-174. https://doi.org/10.3970/mcb.2010.007.165
Vancouver Style
Song G, Luo Q, Xu B, Ju Y. Mechanical stretch-induced changes in cell morphology and mrna expression of tendon/ligament-associated genes in rat bone-marrow mesenchymal stem cells. Mol Cellular Biomechanics . 2010;7(3):165-174 https://doi.org/10.3970/mcb.2010.007.165
IEEE Style
G. Song, Q. Luo, B. Xu, and Y. Ju "Mechanical Stretch-Induced Changes in Cell Morphology and mRNA Expression of Tendon/Ligament-Associated Genes in Rat Bone-Marrow Mesenchymal Stem Cells," Mol. Cellular Biomechanics , vol. 7, no. 3, pp. 165-174. 2010. https://doi.org/10.3970/mcb.2010.007.165



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