Open Access
ARTICLE
Expression Changes, Prognostic Analysis and Risk Factors of miR-625-3p and miR-449a in Osteosarcoma Patients after Surgery
Hui Zhang, Zhan Wang, Lin Liu, Fuqiang Zhang, Yuxin Song, Yaowen Qian*
Department of Orthopaedics, Gansu Provincial Hospital, Lanzhou, 730000, China
* Corresponding Author: Yaowen Qian. Email:
Oncologie 2020, 22(1), 23-33. https://doi.org/10.32604/oncologie.2020.012493
Abstract
The expression changes of miR-625-3p and miR-449a in osteosarcoma
patients after surgery is the critical study in this paper. Analysis of their prognosis
and risk factors can be helpful in understanding the prognostic value for clinical
purposes. Fifty-eight patients with osteosarcoma diagnosed in our hospital were
considered as the research group (RG), and 52 health subjects at the same time
were collected as the control group (CG). Fluorescence quantitative PCR (RTPCR) was employed to test the expression levels of miR-625-3p and miR-449a in
the serum of subjects in both groups before surgery and patients in the RG after
surgery. The survival conditions of both groups with high and low miR-625-3p
and miR-449a expression levels were compared after 5-year follow-up of patients
in the RG. The prognostic factors of osteosarcoma patients were assessed through
Cox regression analysis. The miR-625-3p expression level in the RG was
dramatically higher than that in the CG (
p < 0.05), and the miR-449a expression
level was dramatically lower than that in the CG (
p < 0.05). The miR-625-3p
expression level in the RG after surgery was markedly lower than that before
surgery (
p < 0.05), and the miR-449a expression level was markedly higher than
that before surgery (
p < 0.05). After they were followed up for 5 years, the overall
survival rate of those in the RG was 60.34%, the rate of miR-625-3p low
expression group (LEG) was dramatically higher than that of high expression
group (HEG) (
p < 0.05), and the rate of miR-449a HEG was markedly higher than
that of miR-449a LEG (
p < 0.05). The relative expression of miR-625-3p and miR-
449a was remarkably different from tumor size, lymph node metastasis, and
Enneking stage (
p < 0.05). Univariate Cox regression analysis identified that tumor
size, lymph node metastasis, Enneking stage, miR-625-3p and miR-449a were risk
factors affecting the prognosis of patients; multivariate Cox regression analysis
found that the five were independent prognostic factors of them. miR-625-3p and
miR-449a are differentially expressed in the postoperative serum of osteosarcoma
patients, and their differential expression is an independent risk factor affecting
their prognosis, which has prognostic value and can provide a reference value for
clinical practice.
Keywords
Cite This Article
Zhang, H., Wang, Z., Liu, L., Zhang, F., Song, Y. et al. (2020). Expression Changes, Prognostic Analysis and Risk Factors of miR-625-3p and miR-449a in Osteosarcoma Patients after Surgery.
Oncologie, 22(1), 23–33. https://doi.org/10.32604/oncologie.2020.012493