Open Access
ARTICLE
Icotinib Enhanced Radiosensitization of Nasopharyngeal Carcinoma by Inhibiting the Expression of Epidermal Growth Factor Receptor
Bo Wu1,#, Huacai Xiong2,#, Yong Wang1, Shankun Zhao1, Hongsheng Lu1, Wei Hu3,*
1
Department of Radiotherapy, Taizhou Central Hospital (Taizhou University Hospital), Taizhou, China
2
Department of Radiotherapy, Ningbo Medical Center Lihuili Hospital, Ningbo, China
3
WeiMai Technology Limited Company, Hangzhou, China
* Corresponding Author: Wei Hu. Email:
# These authors contributed equally to this work
Oncologie 2022, 24(3), 553-563. https://doi.org/10.32604/oncologie.2022.023025
Received 06 April 2022; Accepted 21 June 2022; Issue published 19 September 2022
Abstract
Epidermal growth factor receptor (EGFR) is frequently overexpressed in multiple malignancies. Icotinib (IH), a
new EGFR tyrosine kinase inhibitor, enhances radiosensitivity in various types of cancer, but its effect on nasopharyngeal carcinoma (NPC) remains unclear. Total 115 NPC tissue sections and 30 nasopharyngitis tissue sections were enrolled. The correlation of EGFR expression and clinicopathologic features of NPC was analyzed.
Survival analysis was calculated by using univariate and multivariate regression analysis. A radioresistant NPC
cell line, CNE-2R, was established with a gradient irradiation schedule. Cell viability, colony formation and EGFR
expression of CNE-2/2R cells were examined. Significant higher expression of EGFR was observed in NPC tissues
than chronic nasopharyngitis lesions. EGFR expression was significantly correlated with both tumor stage
(
P < 0.001) and tumor-node-metastasis stage of NPC (
P = 0.006). EGFR expression was an independent prognostic factor of disease-free survival (
P = 0.047) and the overall survival of NPC (
P = 0.016). Cell viability was higher
in CNE-2R than CNE-2 on days 1, 2, 4, and 6 after radiation of 4 Gy. The colony number of CNE-2R was significantly higher than that of CNE-2 (
P < 0.05), while IH enhanced the radiosensitizing effect of CNE-2R with
lower survival fraction (
P < 0.05). EGFR mRNA and protein expression levels were significantly higher in
CNE-2R cells compared to CNE-2 cells, but significantly decreased after IH treatment (all
P < 0.05). In conclusion, high EGFR expression is a poor prognostic factor for NPC patients. IH enhances the radiosensitivity of
CNE-2R cells and reduce EGFR expression.
Keywords
Cite This Article
Wu, B., Xiong, H., Wang, Y., Zhao, S., Lu, H. et al. (2022). Icotinib Enhanced Radiosensitization of Nasopharyngeal Carcinoma by Inhibiting the Expression of Epidermal Growth Factor Receptor.
Oncologie, 24(3), 553–563.