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MicroRNA-107 Promotes Proliferation, Migration, and Invasion of Osteosarcoma Cells by Targeting Tropomyosin 1

Rui Jiang*, Chao Zhang, Guangyao Liu*, Rui Gu*, Han Wu*

* Department of Orthopedics, China–Japan Union Hospital of Jilin University, Changchun, Jilin, P.R. China
† Department of Ophthalmology, The Second Hospital of Jilin University, Changchun, Jilin, P.R. China

Oncology Research 2017, 25(8), 1409-1419. https://doi.org/10.3727/096504017X14882829077237

14 June 2024 Editors Note:

Readers are alerted that concerns were raised about the integrity of some of the data presented in the article. Further editorial action will be taken once this matter has been resolved.

Abstract

Osteosarcoma is the most common primary bone malignancy manifested predominantly in children and young adults. Studies indicate that miR-107 is involved in the pathogenesis of osteosarcoma and that tropomyosin 1 (TPM1) acts as a tumor suppressor in many types of cancer. In this study, we analyzed the effect of miR-107 on human osteosarcoma cells and investigated the mechanism in which TPM1 is involved. miR-107 expression in human osteosarcoma tissues and cells was analyzed in quantitative real-time PCR (qRT-PCR). Human osteosarcoma (U2OS) cells were transfected with miR-107 mimic, inhibitor, or scramble controls to evaluate the effect of miR-107 on cellular migration and invasion, cell viability, and apoptosis. Cells were cotransfected with the miR-107 mimic and TPM1 3'-UTR wild-type (wt) recombinant vector or mutant type (mt) as a negative control. The binding effect of miR-107 on TPM1 3'-UTR was determined by dual-luciferase reporter assay. The expression of TPM1, apoptosis-related proteins, and signaling molecules was determined by qRT-PCR and Western blotting. The results showed that miR-107 expression was upregulated in osteosarcoma tissues and cell lines. miR-107 overexpression promoted U2OS cell viability, migration, and invasion whereas it inhibited apoptosis. miR-107 inhibitor transfection ameliorated or abolished these effects after miR-107 binding to TPM1 3'-UTR-wt regulated TPM1 expression. miR-107 in U2OS cells activated MEK/ERK and NF-kB signaling pathways via TPM1. In conclusion, miR-107 overexpression promoted U2OS cell viability, migration, and invasion via downregulation of TPM1 and might be through activating the MEK/ERK and NF-kB signaling pathways.

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Cite This Article

APA Style
Jiang, R., Zhang, C., Liu, G., Gu, R., Wu, H. (2017). Microrna-107 promotes proliferation, migration, and invasion of osteosarcoma cells by targeting tropomyosin 1. Oncology Research, 25(8), 1409-1419. https://doi.org/10.3727/096504017X14882829077237
Vancouver Style
Jiang R, Zhang C, Liu G, Gu R, Wu H. Microrna-107 promotes proliferation, migration, and invasion of osteosarcoma cells by targeting tropomyosin 1. Oncol Res. 2017;25(8):1409-1419 https://doi.org/10.3727/096504017X14882829077237
IEEE Style
R. Jiang, C. Zhang, G. Liu, R. Gu, and H. Wu "MicroRNA-107 Promotes Proliferation, Migration, and Invasion of Osteosarcoma Cells by Targeting Tropomyosin 1," Oncol. Res., vol. 25, no. 8, pp. 1409-1419. 2017. https://doi.org/10.3727/096504017X14882829077237



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