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ARTICLE
CHRM3 is a novel prognostic factor of poor prognosis and promotes glioblastoma progression via activation of oncogenic invasive growth factors
BIN ZHANG1,#, JIANYI ZHAO3,#, YONGZHI WANG2,#, HUA XU1, BO GAO1, GUANGNING ZHANG1, BIN HAN1, GUOHONG SONG1, JUNCHEN ZHANG1,*, WEI MENG1,*
1 Department of Neurosurgery, Affiliated Hospital of Jining Medical University, Jining, China
2 Department of Neurosurgery, The City Peoples’ Hospital of Fuyang, Fuyang, China
3 Brain Injury Center, Department of Neurosurgery, Ren Ji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
* Corresponding Authors: Junchen Zhang, ; Wei Meng,
# These authors contributed equally to this work
(This article belongs to the Special Issue: Signaling Pathway Crosstalk in Malignant Tumors: Molecular Targets and Combinatorial Therapeutics)
Oncology Research 2023, 31(6), 917-927. https://doi.org/10.32604/or.2023.030425
Received 05 April 2023; Accepted 26 June 2023; Issue published 15 September 2023
Abstract
Glioblastoma (GBM) is the most aggressive cancer of the brain and has a high mortality rate due to the lack of
effective treatment strategy. Clarification of molecular mechanisms of GBM’s characteristic invasive growth are urgently
needed to improve the poor prognosis. Single-nuclear sequencing of primary and recurrent GBM samples revealed that
levels of M3 muscarinic acetylcholine receptor (CHRM3) were significantly higher in the recurrent samples than in the
primary samples. Moreover, immunohistochemical staining of an array of GBM samples showed that high levels of
CHRM3 correlated with poor prognosis, consistent with The Cancer Genome Atlas database. Knockdown of CHRM3
inhibited GBM cell growth and invasion. An assay of orthotopic GBM animal model
in vivo indicated that inhibition
of CHRM3 significantly suppressed GBM progression with prolonged survival time. Transcriptome analysis revealed
that CHRM3 knockdown significantly reduced an array of classic factors involved in cancer invasive growth,
including MMP1/MMP3/MMP10/MMP12 and CXCL1/CXCL5/CXCL8. Taken together, CHRM3 is a novel and vital
factor of GBM progression via regulation of multiple oncogenic genes and may serve as a new biomarker for
prognosis and therapy of GBM patients.
Keywords
Cite This Article
APA Style
ZHANG, B., ZHAO, J., WANG, Y., XU, H., GAO, B. et al. (2023). CHRM3 is a novel prognostic factor of poor prognosis and promotes glioblastoma progression via activation of oncogenic invasive growth factors. Oncology Research, 31(6), 917-927. https://doi.org/10.32604/or.2023.030425
Vancouver Style
ZHANG B, ZHAO J, WANG Y, XU H, GAO B, ZHANG G, et al. CHRM3 is a novel prognostic factor of poor prognosis and promotes glioblastoma progression via activation of oncogenic invasive growth factors. Oncol Res. 2023;31(6):917-927 https://doi.org/10.32604/or.2023.030425
IEEE Style
B. ZHANG et al., "CHRM3 is a novel prognostic factor of poor prognosis and promotes glioblastoma progression via activation of oncogenic invasive growth factors," Oncol. Res., vol. 31, no. 6, pp. 917-927. 2023. https://doi.org/10.32604/or.2023.030425