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LncRNA PCGEM1 facilitates cervical cancer progression via miR-642a-5p/KIF5B axis

YUANLIN LIU1,3,#, YAN LIU2,#, YAN WANG2, QIANG WANG2, YAN YAN1, DANDAN ZHANG2,*, HUIQIN LIU2,*

1 Department of Obstetrics and Gynecology, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji University, Shanghai, 200092, China
2 Department of Obstetrics and Gynecology, The Second People’s Hospital of Nantong City, Nantong, 226002, China
3 Department of Obstetrics and Gynecology, Affiliated Hospital of Nantong University, Nantong, 226002, China

* Corresponding Authors: DANDAN ZHANG. Email: email; HUIQIN LIU. Email: email

Oncology Research 2024, 32(7), 1221-1229. https://doi.org/10.32604/or.2024.047454

Abstract

At present, the role of many long non-coding RNAs (lncRNAs) as tumor suppressors in the formation and development of cervical cancer (CC) has been studied. However, lncRNA prostate cancer gene expression marker 1 (PCGEM1), whose high expression not only aggravates ovarian cancer but also can induce tumorigenesis and endometrial cancer progression, has not been studied in CC. The objective of this study was to investigate the expression and the underlying role of PCGEM1 in CC. The relative expression of PCGEM1 in CC cells was detected by real-time PCR. After the suppression of PCGEM1 expression by shRNA, the changes in the proliferation, migration, and invasion capacities were detected via CCK-8 assay, EdU assay, and colony formation assay wound healing assay. Transwell assay and the changes in expressions of epithelial-to-mesenchymal transition (EMT) markers were determined by western blot and immunofluorescence. The interplay among PCGEM1, miR-642a-5p, and kinesin family member 5B (KIF5B) was confirmed by bioinformatics analyses and luciferase reporter assay. Results showed that PCGEM1 expressions were up-regulated within CC cells. Cell viabilities, migration, and invasion were remarkably reduced after the suppression of PCGEM1 expression by shRNA in Hela and SiHa cells. N-cadherin was silenced, but E-cadherin expression was elevated by sh-PCGEM1. Moreover, by sponging miR-642a-5p in CC, PCGEM1 was verified as a competitive endogenous RNA (ceRNA) that modulates KIF5B levels. MiR-642a-5p down-regulation partially rescued sh-PCGEM1’s inhibitory effects on cell proliferation, migration, invasion, and EMT process. In conclusion, the PCGEM1/miR-642a-5p/KIF5B signaling axis might be a novel therapeutic target in CC. This study provides a research basis and new direction for targeted therapy of CC.

Graphic Abstract

LncRNA PCGEM1 facilitates cervical cancer progression via miR-642a-5p/KIF5B axis

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APA Style
LIU, Y., LIU, Y., WANG, Y., WANG, Q., YAN, Y. et al. (2024). Lncrna PCGEM1 facilitates cervical cancer progression via mir-642a-5p/kif5b axis. Oncology Research, 32(7), 1221-1229. https://doi.org/10.32604/or.2024.047454
Vancouver Style
LIU Y, LIU Y, WANG Y, WANG Q, YAN Y, ZHANG D, et al. Lncrna PCGEM1 facilitates cervical cancer progression via mir-642a-5p/kif5b axis. Oncol Res. 2024;32(7):1221-1229 https://doi.org/10.32604/or.2024.047454
IEEE Style
Y. LIU et al., "LncRNA PCGEM1 facilitates cervical cancer progression via miR-642a-5p/KIF5B axis," Oncol. Res., vol. 32, no. 7, pp. 1221-1229. 2024. https://doi.org/10.32604/or.2024.047454



cc This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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