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  • Open Access


    AFLP polymorphism in restored provenances of Ceiba aesculifolia within an urban heat island

    Olvera-Mendoza EI1, SI Lara-Cabrera1, C Sáenz-Romero2, R Lindig-Cisneros3

    Phyton-International Journal of Experimental Botany, Vol.85, pp. 169-175, 2016, DOI:10.32604/phyton.2016.85.169

    Abstract To quantify the effect on genetic diversity of restoring tree populations in an area under the influence of an urban heat island, we evaluated the genetic diversity of 72 Ceiba aesculifolia individuals, from a restoration experiment established from 2170 to 2260 m a.s.l. Reintroduced individuals were compared with the provenance from which the seeds were obtained, and two external provenances, for a total of 123 individuals. Samples were analyzed with three AFLP primer combinations. Polymorphisms of 38.4 to 62.5% were obtained. Genetic diversity estimated with the Simpson index ranged from 0.14 to 0.2. The provenances in the restoration site had… More >

  • Open Access


    Cladistics of Mexican nopal genotypes (Opuntia spp.) based on AFLP markers and fruit characters

    Espinoza Sánchez EA1, H Silos Espino1, S Flores Benitez1, LL Valera Montero1, E Rodríguez Salazar2, C Gallegos Vázquez3, F Guevara Lara4, M González Chavira5, HS Guzmán Maldonado5

    Phyton-International Journal of Experimental Botany, Vol.83, pp. 299-306, 2014, DOI:10.32604/phyton.2014.83.299

    Abstract Dendrograms were constructed based on AFLP markers and a table was used to group fruit characters of wild and cultivated genotypes of Mexican nopal (Opuntia spp.). The AFLP dendrogram obtained using ≈57.5 markers (from primers Mse I-CAC/Eco RI-AAG) did not agree with the known taxonomic classification for this species. Additionally, a complementary table for fruit characters (morphological and °Brix) showed a wide range for wild genotypes, e.g. from 21-22 °Brix for ‘Tapón Blanco’ and ‘Guilanche’ genotypes, to 5.0-8.2 °Brix for xoconostles (acidic fruits). Even though there were some divergences with taxonomic classification, it is expected that both genetic and physicochemical… More >

  • Open Access


    AFLP marker application to explore heterosis in Capsicum spp.

    Castañón-Najera G1, M Ramírez-Meraz3, R Ruiz-Salazar4, N Mayek-Pérez2

    Phyton-International Journal of Experimental Botany, Vol.80, pp. 53-58, 2011, DOI:10.32604/phyton.2011.80.053

    Abstract The chili varieties: (1) Mirador, (2) Piquín huasteco, (3) Pico paloma, (4) Ozulamero and (5) Chilpaya Tabasco were crossed in a diallel without reciprocal crosses to produce 10 F1 hybrid combinations. While the previous varieties (1), (2) and (4) belong to Capsicum annuum varieties, (3) and (5) pertain to Caspicum frutescens. The four study AFLP markers amplified 282 bands or fragments, and of these, 264 were polymorphic. The estimated Genetic Parameters showed that there was dominance based on the genetic distances in the study DNA fragments. Heritability, which was estimated in a narrow sense, was moderate (0.53). This indicated the… More >

  • Open Access


    Estimation of the genetic diversity of Opuntia spp. using molecular markers AFLP

    García-Zambrano2 EA, F Zavala-García2, A Gutiérrez-Diez, MC Ojeda-Zacarías, I Cerda-Hurtado3

    Phyton-International Journal of Experimental Botany, Vol.78, pp. 117-120, 2009, DOI:10.32604/phyton.2009.78.117

    Abstract The objective of this study was to apply the AFLP-type molecular marker technique to estimate genetic diversity in cactus pear within the Germplasm Bank of FAUANL. Twelve accessions have been reported as possible duplicates through type RAPD- molecular markers in such Bank. DNA was extracted using the cellular ruptor technique, and then digested and bound. A preamplification and subsequent selective amplification were conducted. Amplified fragments were then separated and analyzed. It was concluded that none of the accessions was duplicated. This was because 8 groups were formed in the dendogram obtained after applying the UPGMA method. There were 3 accessions… More >

  • Open Access


    Molecular differentiation of Xoconostle (Opuntia spp.) using AFLP molecular markers

    García-Zambrano EA1, MC Ojeda-Zacarías1, JG Gutiérrez-Soto1, SR Sinagawa-García1, A Gutiérrez-Díez1, J Martínez-de la Cerda1, JA Pedroza-Flores1, C Gallegos-Vázquez2, JI García-Zambrano3

    Phyton-International Journal of Experimental Botany, Vol.87, pp. 87-93, 2018, DOI:10.32604/phyton.2018.87.087

    Abstract The technologies based on molecular markers of DNA are the most advanced and possibly the most effective and accurate means to identify genetic variation. The objective of this study was to use molecular genetic markers namely AFLP as a tool for the molecular differentiation of 36 accessions of xoconostle collected in the Centro Regional Universitario Centro Norte of the Universidad Autónoma de Chapingo (CRUCEN-UACh.). DNA extraction was performed by the method of cellular ruptor using FastDNA® Kit, and quantification of purity and yield of DNA was carried out through the Nanodrop 2000. It was observed that the DNA concentrations ranged… More >

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