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Cladistics of Mexican nopal genotypes (Opuntia spp.) based on AFLP markers and fruit characters

Espinoza Sánchez EA1, H Silos Espino1, S Flores Benitez1, LL Valera Montero1, E Rodríguez Salazar2, C Gallegos Vázquez3, F Guevara Lara4, M González Chavira5, HS Guzmán Maldonado5
1 Laboratorio de Biotecnología Aplicada, Instituto Tecnológico El Llano, Aguascalientes, Km 18 carretera Aguascalientes-San Luis Potosí, México, C.P. 20330.
2 Centro de Bachillerato Tecnológico Agropecuario No. 138, Km 1, carretera Villa Hidalgo-Pinos, Zacatecas, México, C.P. 98980.
3 Depositario Nacional de Opuntia, Centro Regional Universitario Centro Norte de la Universidad Autónoma de Chapingo. Cruz del Sur # 100, Colonia Constelación, El Orito, Zacatecas, México C.P. 98085.
4 Laboratorio de Biotecnología y Funcionalidad de Alimentos, Universidad Autónoma de Aguascalientes, Av. Universidad No. 940, Aguascalientes, Aguascalientes., México, C.P. 20100.
5 Campo Experimental Bajío, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Km 6.5, Carretera Celaya-San Miguel de Allende, Roque, Celaya Guanajuato. México, C.P. 38110.
* Corresponding Author:Address Correspondence to: Héctor Silos Espino, e-mail:

Phyton-International Journal of Experimental Botany 2014, 83(all), 299-306. https://doi.org/10.32604/phyton.2014.83.299

Abstract

Dendrograms were constructed based on AFLP markers and a table was used to group fruit characters of wild and cultivated genotypes of Mexican nopal (Opuntia spp.). The AFLP dendrogram obtained using ≈57.5 markers (from primers Mse I-CAC/Eco RI-AAG) did not agree with the known taxonomic classification for this species. Additionally, a complementary table for fruit characters (morphological and °Brix) showed a wide range for wild genotypes, e.g. from 21-22 °Brix for ‘Tapón Blanco’ and ‘Guilanche’ genotypes, to 5.0-8.2 °Brix for xoconostles (acidic fruits). Even though there were some divergences with taxonomic classification, it is expected that both genetic and physicochemical information would contribute to a more appropriate identification of Opuntia genotypes that, in turn, may be used in breeding programs of this genus.

Keywords

Molecular Characterization, Opuntia, AFLP, Dendrogram, Fruit morphology.

Cite This Article

EA, E. S., Espino, H. S., Benitez, S. F., Montero, L. V., Salazar, E. R. et al. (2014). Cladistics of Mexican nopal genotypes (Opuntia spp.) based on AFLP markers and fruit characters. Phyton-International Journal of Experimental Botany, 83(all), 299–306.



This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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