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  • Open Access


    Vital Parameters Assessments of Starvation Tolerance of in vitro Populus alba Culture

    Mohamed Fathy Ahmed1, Eman Zakaria Ahmed2, Salman Aloufi3, Mohammed Alqurashi3, Amal Alyamani3, Eman Fayad3, Eman Tawfik Hussien2,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.4, pp. 889-902, 2022, DOI:10.32604/phyton.2022.018600

    Abstract Populus alba is a large woody deciduous plant. The plant has been introduced to shooting, then multiplication of rooting on Murashige and Skoog (MS) medium. This work was designed to estimate the effect of two factors (low levels of 1-Naphthaleneacetic acid NAA and sucrose) on P. alba response resulting in 6 treatments compared to the control, with twelve measured responses. There was a significant difference in some measurements in morphology, like plantlets fresh-weight, shoot-, root-length, and leaf number. In the physiological measurements, there were significant differences in all the measured parameters. The low concentrations of sucrose and media composition/power (MS… More >

  • Open Access


    Genetic stability in rice micropropagation


    BIOCELL, Vol.28, No.1, pp. 13-20, 2004, DOI:10.32604/biocell.2004.28.013

    Abstract An efficient clonal propagation procedure for six rice varieties cultivated in Argentina was developed by using shoot tip cultures, and the genetic stability of the micropropagated plants was verified by isozyme analysis. One week old seedlings obtained on MS medium were sectioned and subcultured on MS medium (0.75% agar) supplemented with different combination and concentrations of cytokinins (BAP and KIN) and auxins (2,4-D and NAA). After four weeks of culture, multiple shoots were obtained. The best response was observed on MS supplemented with BAP 5 mg l-1. Shoot clumps were multiplied in MS liquid medium containing BAP 5 mg l-1.… More >

  • Open Access


    Genetic and resistance stability to Black Sigatoka disease during micropopagation of Musa CIEN BTA-03 somaclonal variant

    Giménez1 C, E de García2, O Haddad3

    Phyton-International Journal of Experimental Botany, Vol.77, pp. 65-79, 2008, DOI:10.32604/phyton.2008.77.065

    Abstract Evaluation of clonal micropropagation and phenotype stability of elite somaclones are critical steps for development of new varieties. In the present work somaclon variant CIEN BTA-03 (resistant to Black Sigatoka), obtained through in vitro process from cultivar Williams (susceptible to Black Sigatoka), was micropropagated via apical shoot culture for five multiplication cycles in 0.5 mg/l of benzyl-aminopurine (BA). To verify the genetic stability of the progeny of this elite material, random amplified polymorphic DNA (RAPD) markers were used. A total of 5,292 monomorphic bands were obtained from the amplification of fifty six DNA samples (extracted from vitroplants randomly selected) with… More >

  • Open Access


    Stability of protein patterns in coffee seedlings regenerated by somatic embryogenesis

    Menéndez-Yuffá1 A, L Ríos-Bolívar2

    Phyton-International Journal of Experimental Botany, Vol.77, pp. 49-64, 2008, DOI:10.32604/phyton.2008.77.049

    Abstract Regeneration and multiplication of coffee (Coffea arabica L.) through somatic embryogenesis can be efficiently achieved through different methods. However, it is very important to analyze the progenies obtained by these methods in order to establish their genetic stability. The objectives of this research were (1) to determine the protein electrophoretic patterns in leaves of coffee vitroplants which were regenerated through somatic embryogenesis, and (2) to compare these patterns with those of the explant donor plants. The protein patterns of some coffee genotypes were initially determined to detect possible differences among them. Each analyzed genotype showed a characteristic set of protein… More >

  • Open Access


    Genetic stability of a synthetic variety

    Rodríguez-Pérez JE, J Sahagún-Castellanos, JL Escalante-González, JJ López-Reynoso, C V illanueva-Verduzco

    Phyton-International Journal of Experimental Botany, Vol.86, pp. 224-227, 2017, DOI:10.32604/phyton.2017.86.224

    Abstract Synthetic varieties (SVs) have been assumed to be genetically stable populations through generations. However, it has been recognized that the randomness of the genetic mechanism, the presence of parents with heterozygous genotypes, and the finite sample sizes of the individuals that represent each parent of a SV may cause gene loss, which may make it difficult to obtain the expected genotypic array of a given SV. To study this issue, the number of non-identical by descent (NIBD) genes in the sample of m plants of each parental line was considered as a random variable (Ym). The objectives were: (1) to… More >

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