Mohamed Fathy Ahmed¹, Eman Zakaria Ahmed², Salman Aloufi³, Mohammed Alqurashi³, Amal Alyamani³, Eman Fayad³, Eman Tawfik Hussien^2,*

Phyton-International Journal of Experimental Botany, Vol.91, No.4, pp. 889-902, 2022, DOI:10.32604/phyton.2022.018600

Abstract Populus alba is a large woody deciduous plant. The plant has been introduced to shooting, then multiplication of rooting on Murashige and Skoog (MS) medium. This work was designed to estimate the effect of two factors (low levels of 1-Naphthaleneacetic acid NAA and sucrose) on P. alba response resulting in 6 treatments compared to the control, with twelve measured responses. There was a significant difference in some measurements in morphology, like plantlets fresh-weight, shoot-, root-length, and leaf number. In the physiological measurements, there were significant differences in all the measured parameters. The low concentrations of sucrose and media composition/power (MS… More >

R. MEDINA, M. FALOCI, M.A. MARASSI, AND L.A. MROGINSKI.

BIOCELL, Vol.28, No.1, pp. 13-20, 2004, DOI:10.32604/biocell.2004.28.013

Abstract An efficient clonal propagation procedure for six rice varieties cultivated in Argentina was developed by using shoot tip cultures, and the genetic stability of the micropropagated plants was verified by isozyme analysis. One week old seedlings obtained on MS medium were sectioned and subcultured on MS medium (0.75% agar) supplemented with different combination and concentrations of cytokinins (BAP and KIN) and auxins (2,4-D and NAA). After four weeks of culture, multiple shoots were obtained. The best response was observed on MS supplemented with BAP 5 mg l^-1. Shoot clumps were multiplied in MS liquid medium containing BAP 5 mg l^-1.… More >

Giménez¹ C, E de García², O Haddad³

Phyton-International Journal of Experimental Botany, Vol.77, pp. 65-79, 2008, DOI:10.32604/phyton.2008.77.065

Abstract Evaluation of clonal micropropagation and phenotype stability of elite somaclones are critical steps for development of new varieties. In the present work somaclon variant CIEN BTA-03 (resistant to Black Sigatoka), obtained through in vitro process from cultivar Williams (susceptible to Black Sigatoka), was micropropagated via apical shoot culture for five multiplication cycles in 0.5 mg/l of benzyl-aminopurine (BA). To verify the genetic stability of the progeny of this elite material, random amplified polymorphic DNA (RAPD) markers were used. A total of 5,292 monomorphic bands were obtained from the amplification of fifty six DNA samples (extracted from vitroplants randomly selected) with… More >

Menéndez-Yuffá¹ A, L Ríos-Bolívar²

Phyton-International Journal of Experimental Botany, Vol.77, pp. 49-64, 2008, DOI:10.32604/phyton.2008.77.049

Abstract Regeneration and multiplication of coffee (Coffea arabica L.) through somatic embryogenesis can be efficiently achieved through different methods. However, it is very important to analyze the progenies obtained by these methods in order to establish their genetic stability. The objectives of this research were (1) to determine the protein electrophoretic patterns in leaves of coffee vitroplants which were regenerated through somatic embryogenesis, and (2) to compare these patterns with those of the explant donor plants. The protein patterns of some coffee genotypes were initially determined to detect possible differences among them. Each analyzed genotype showed a characteristic set of protein… More >

Rodríguez-Pérez JE, J Sahagún-Castellanos, JL Escalante-González, JJ López-Reynoso, C V illanueva-Verduzco

Phyton-International Journal of Experimental Botany, Vol.86, pp. 224-227, 2017, DOI:10.32604/phyton.2017.86.224

Abstract Synthetic varieties (SVs) have been assumed to be genetically stable populations through generations. However, it has been recognized that the randomness of the genetic mechanism, the presence of parents with heterozygous genotypes, and the finite sample sizes of the individuals that represent each parent of a SV may cause gene loss, which may make it difficult to obtain the expected genotypic array of a given SV. To study this issue, the number of non-identical by descent (NIBD) genes in the sample of m plants of each parental line was considered as a random variable (Ym). The objectives were: (1) to… More >