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  • Open Access

    ARTICLE

    Torin 1, TOR Inhibitor Enhances Cellular Proliferation in NT-1 Tobacco Suspension Cell Cultures

    Grisel Fierros-Romero1,*, M. Elena Mellado-Rojas2, Reynaldo C. Pless3, Juan Ignacio Valiente-Banuet1, Ashutosh Sharma1 and Elda M. Beltrán-Peña2

    Phyton-International Journal of Experimental Botany, Vol.88, No.2, pp. 131-137, 2019, DOI:10.32604/phyton.2019.05036

    Abstract Torin 1 is an ATP-competitive TOR inhibitor which inhibits the signaling of TOR and S6K kinase in mammals and plants. The objective of this research is to determine the effect of Torin 1 in a relatively simple and homogeneous plant system such as the NT-1 tobacco suspension cell cultures. Cultures of NT-1 cells were tested with 5, 50, 150 and 250 nM of Torin 1. During kinetics growth of NT-1 tobacco suspension cell cultures, 150 and 250 nM Torin 1 inhibits the early growth and later enhanced the cellular proliferation during exponential growth by means More >

  • Open Access

    ARTICLE

    First report of Tobacco mosaic virus (TMV) and Poinsettia mosaic virus (PnMV) in poinsettia (Euphorbia pulcherrima Willd. Ex Klotzch) in Mexico

    Ocampo Ocampo T1, DL Ochoa Martínez1, S Ramírez Rojas2, G Valdovinos Ponce1, C Nava Díaz1

    Phyton-International Journal of Experimental Botany, Vol.82, pp. 235-241, 2013, DOI:10.32604/phyton.2013.82.235

    Abstract The Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), in Zacatepec Morelos, started a genetic improvement program of wild and semi-cultivated poinsettia plants ("nochebuena de sol"). It is important to generate a phytosanitary database that allows the release of new varieties and hybrids that provide tolerance to the major pathogens reported in cultivated poinsettia plants. The phytosanitary status of the “nochebuena de sol” plants is not available in Mexico, so the objective of this research was to generate preliminary data about viruses associated to these materials. Based on DAS-ELISA and RT-PCR results, we reported More >

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