Huimin Cui, Shuo Qu, Abraham Lamboro, Yaolei Jiao, Piwu Wang^*

Phyton-International Journal of Experimental Botany, Vol.91, No.7, pp. 1445-1464, 2022, DOI:10.32604/phyton.2022.020010

Abstract In agricultural production, a single insect-resistant and disease-resistant variety can no longer meet the demand. In this study, the expression vector pCAMBIA-3301-PR1 containing the disease-resistant gene PR1 was constructed by means of genetic engineering, and the PR1 gene was genetically transformed to contain the PR1 gene through the pollen tube method. In CryAb-8Like transgenic high-generation T₇ receptor soybean, a new material that is resistant to insects and diseases is obtained. For T₂ transformed plants, routine PCR detection, Southern Blot hybridization, fluorescence quantitative PCR detection, indoor and outdoor pest resistance identification and indoor disease resistance identification were performed. The results showed… More >

Yajun Li^1,#, Yumeng Cui^1,#, Bingjie Wang¹, Yue Li¹, Mengmeng Zhang², Silan Dai¹, He Huang^1,*

Phyton-International Journal of Experimental Botany, Vol.91, No.4, pp. 869-888, 2022, DOI:10.32604/phyton.2022.018659

Abstract Genetic transformation is one of the key steps in the molecular breeding of chrysanthemum, which relies on an optimal regeneration and transformation system. However, the regeneration system of different chrysanthemum cultivars varies, and the regeneration time of most cultivars is long. To screen cultivars with highly efficient regeneration, leaves and shoot tip thin cell layers (tTCL) from eight chrysanthemum cultivars with different flower colors and flower types were cultured on Murashige and Skoog media (MS) supplemented with 1.0–5.0 mg L⁻¹ 6-benzylaminopurine (6-BA) and 0.1–1.0 mg L⁻¹ α-naphthaleneacetic (NAA). The results showed that the most efficient regeneration media were MS +… More >

Yizhao Huang¹, Nannan Li², Deya Wang⁴, Jian Du¹, Weitang Liu³, Jinxin Wang^3,*, Wei Li^1,*

Phyton-International Journal of Experimental Botany, Vol.91, No.4, pp. 761-772, 2022, DOI:10.32604/phyton.2022.018462

Abstract Beckmannia syzigachne is a noxious weed for rice-wheat rotations in China. The B. syzigachne (AH-02) population evolved metabolic resistance to fenoxaprop-P-ethyl and mesosulfuron-methyl. To investigate the function of GT73C1 in this population, the GT73C1 gene was amplified by reverse transcription-polymerase chain reaction, and the sequence was 100% consistent with the transcriptome data. Its phylogenetic tree was displayed and annotated using FigTree v1.4.4. The plant overexpression vector of GT73C1 gene was constructed and used to transform Brachypodium distachyon plants. Furthermore, the expression of GT73C1 was significantly induced by fenoxaprop-P-ethyl and mesosulfuron-methyl, which was consistent with the findings from the whole plant… More >

Mengtong Liu¹, Yangyang Zhou¹, Tongyu Liu¹, Jianyu Lu¹, Jing Qu², Shuyan Guan^2,*, Yiyong Ma^2,*

Phyton-International Journal of Experimental Botany, Vol.91, No.2, pp. 363-374, 2022, DOI:10.32604/phyton.2022.016325

Abstract Immature embryos of inbred maize (Zea mays) lines (H8183, H8184, and H8185) were used for Agrobacterium infection. We used the β-glucuronidase gene (GUS) as the target gene and the glufosinate resistance gene (bar) as the selection marker. We conducted research on several aspects, such as different genotypes, coculture conditions, screening agent concentrations, and concentrations of indole-3-butytric acid (IBA), 6-benzylaminopurine (6-BA), and ascorbic acid (Vc) in the differentiation medium. We optimized the genetic transformation system, and the obtained results indicated that among the three lines studied, the induction rate of H8185 was the highest at 93.2%, followed by H8184, with H8183… More >