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    In vitro propagation of Opuntia ellisiana Griff. and acclimatization to field conditions


    BIOCELL, Vol.26, No.3, pp. 319-324, 2002, DOI:10.32604/biocell.2002.26.319

    Abstract The genus Opuntia is a valuable forage resource in arid and semiarid lands during periods of drought and shortage of herbaceous plants. However, absolute minimum temperatures in the plains of Mendoza represent a limiting factor to cultivate several species.
    Opuntia ellisiana is a cold hardy species, so the goals of this study were to massively propagate it using in vitro culture techniques, and then to acclimatize plantlets obtained to field conditions.
    Different sterilization protocols were tested. Areoles were isolated in laminar airflow cabinet, and cultured on Murashige-Skoog medium, supplemented with sucrose and different BAP and IBA combinations.… More >

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