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  • Open Access


    Osmotic adjustment in Leymus cinereus cv. “Trailhead” under field conditions

    Torres1 YA, CA Busso1, OA Montenegro2, HD Giorgetti2, HD Rodríguez2, D Bentivegna3

    Phyton-International Journal of Experimental Botany, Vol.79, pp. 195-198, 2010, DOI:10.32604/phyton.2010.79.195

    Abstract Osmotic adjustment has been recognized to be an important adaptive response to water stress in higher plants. Although Leymus cinereus cv. “Trailhead” is reputedly a very drought-tolerant rangeland plant, its ability to adjust osmotically in the field has not previously been reported. This study examined the occurrence or not of osmotic adjustment in field-grown plants of Leymus cinereus cv. “Trailhead” during late spring in an arid zone of Patagonia. Plants of this species had been established in the field three years before. With this purpose, leaf laminae of this species were collected and pressure-volume curves made. Results showed turgor maintenance… More >

  • Open Access


    Use of the pressure chamber and thermocouple psychrometers to determine the water relations of plant tissues

    Busso CA

    Phyton-International Journal of Experimental Botany, Vol.77, pp. 327-350, 2008, DOI:10.32604/phyton.2008.77.327

    Abstract Advantages, disadvantages and precautions of using the pressure chamber and thermocouple psychrometers for determinations of plant water potential, and osmotic and turgor pressures are discussed. Comparison of results obtained with both instruments will be influenced by the sampling technique, consistency in both sampling and measurement procedures, and errors inherent to the method itself. In-situ readings of either plant or soil water potential with thermocouple psychrometers are perhaps worthless, or worse (they may even be misleading). Use of thermocouple psychrometers is strictly limited to the laboratory where isothermal conditions can be obtained. The method to determine the water relations of plant… More >

  • Open Access


    Effect of PEG8000 and NaCl on germination and seedling traits of tropical maize (Zea mays L.)

    Castañón-Nájera G1, L Latorunerie-Moreno2, YA Gálvez Muñoz3

    Phyton-International Journal of Experimental Botany, Vol.86, pp. 290-295, 2017, DOI:10.32604/phyton.2017.86.290

    Abstract Among the different types of abiotic stresses, water is the most damaging for crops. In the current research, the effect of water stress, induced with Polyethylene Glycol 8000 (PEG8000) and Sodium chloride (NaCl) solutions, was studied in maize genotypes. A randomized block design (RBD) with a factorial arrangement and four replications of 25 seeds each was used. The studied maize genotypes were RC2C39, RC2C51 (wild varieties of the State of Tabasco backcrossed with the endogamic line CML247 of the International Center for the Improvement of Maize and Wheat), wild Tamulte, VS536, and Asgrow 7573 hybrid, study osmotic pressures (OP) were… More >

  • Open Access


    Osmotic Loading of in Situ Chondrocytes in Their Native Environment

    Rami K Korhonen∗,†, Sang-Kuy Han, Walter Herzog

    Molecular & Cellular Biomechanics, Vol.7, No.3, pp. 125-134, 2010, DOI:10.3970/mcb.2010.007.125

    Abstract Changes in the osmotic environment cause changes in volume of isolated cells and cells in tissue explants, and the osmotic environment becomes hypotonic in cartilage diseases such as osteoarthritis (OA). However, it is not known how cells respond to a hypotonic osmotic challenge when situated in the fully intact articular cartilage.
    A confocal laser scanning microscope was used to image chondrocytes of intact rabbit patellae in an isotonic (300 mOsm) and hypotonic (172 mOsm) immersion medium. Cell volumes were calculated before and 5, 15, 60, 120 and 240 minutes after the change in saline concentration. Local tissue strains and swelling… More >

  • Open Access


    Changes in Triphasic Mechanical Properties of Proteoglycan-Depleted Articular Cartilage Extracted from Osmotic Swelling Behavior Monitored Using High-Frequency Ultrasound

    Q Wang*, YP Zheng∗,†, HJ Niu∗,‡

    Molecular & Cellular Biomechanics, Vol.7, No.1, pp. 45-58, 2010, DOI:10.3970/mcb.2010.007.045

    Abstract This study aims to obtain osmosis-induced swelling strains of normal and proteoglycan (PG) depleted articular cartilage using an ultrasound system and to investigate the changes in its mechanical properties due to the PG depletion using a layered triphasic model. The swelling strains of 20 cylindrical cartilage-bone samples collected from different bovine patellae were induced by decreasing the concentration of bath saline and monitored by the ultrasound system. The samples were subsequently digested by a trypsin solution for approximately 20 min to deplete proteoglycans, and the swelling behaviors of the digested samples were measured again. The bi-layered triphasic model proposed in… More >

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