Home / Advanced Search

  • Title/Keywords

  • Author/Affliations

  • Journal

  • Article Type

  • Start Year

  • End Year

Update SearchingClear
  • Articles
  • Online
Search Results (7)
  • Open Access


    A New Micropropagation Technology of Tilia amurensis: In Vitro Micropropagation of Mature Zygotic Embryos and the Establishment of a Plant Regeneration System

    Shijie Lin1, Zimo Wang1, Hongbo Zhu2, Conghui Wang3, Hongfeng Wang2, Dawei Zhang1, Tianbing Gou1, Guangdao Bao1, Ye Luo1, Huaijiang He1, Zhonghui Zhang1,*

    Phyton-International Journal of Experimental Botany, Vol.93, No.2, pp. 277-289, 2024, DOI:10.32604/phyton.2024.046989

    Abstract Tilia amurensis is an economically valuable broadleaf tree species in Northeast China. The production of high-quality T. amurensis varieties at commercial scales has been greatly limited by the low germination rates. There is thus a pressing need to develop an organogenesis protocol for in vitro propagation of T. amurensis to alleviate a shortage of high-quality T. amurensis seedlings. Here, we established a rapid in vitro propagation system for T. amurensis from mature zygotic embryos and analyzed the effects of plant growth regulators and culture media in different stages. We found that Woody plant medium (WPM) was the optimal primary culture… More >

  • Open Access


    Transcriptome Analysis and Genes Function Verification of Root Development of Paeonia suffruticosa under Sandy Loam Cultivation

    Yinglong Song1,#, Wenqian Shang1,#, Zheng Wang1,*, Songlin He1,2,*, Xinya Meng3, Liyun Shi1, Yuxiao Shen1, Dan He1, Xueyuan Lou1, Yuke Sun1

    Phyton-International Journal of Experimental Botany, Vol.91, No.12, pp. 2791-2812, 2022, DOI:10.32604/phyton.2022.023572

    Abstract Relatively poor in vitro rooting has limited the large-scale commercial production of tree peony. In this study, on the basis of transcriptome sequencing, differentially expressed genes and the associated metabolic pathways were identified in tree peony roots at different stages of root formation under sandy loam cultivation. A total of 31.63 Gb raw data were generated and 120,188 unigenes (mean length of 911.98 bp) were annotated according to six databases (NR, NT, GO, KEGG, COG, and Swiss-Prot). Analyses of the ungerminated root primordium period, induced root primordium period, and root formation period detected 8,232, 6,907, and 10,687 differentially expressed genes related… More >

  • Open Access


    Optimization of Factors Influencing Adventitious Rooting in Hybrid Larch

    Kuipeng Li1,2, Hua Han1, Yunhui Xie1, Xiaomei Sun1,*

    Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 583-593, 2021, DOI:10.32604/phyton.2021.013912

    Abstract Optimization of in vitro adventitious root induction contributes to the development of a large-scale production system of hybrid clone seedlings of larch (Larix spp.). We used orthogonal testing to investigate the factors that affect the rooting of hybrid larch shoots–activated carbon, vitamin B1, sucrose, glycine, glutamic acid (Glu), cysteine, pH, and induction time in the dark and indole-3-butyric acid (IBA). Variance analysis showed that the effects of sucrose concentration on rooting rate, survival rate, and average root number, pH on the average number of roots; and Glu on survival rate were all significant (p < 0.05) when compared to the… More >

  • Open Access


    Brief Note : Plant regeneration after long term callus culture in clones of Asparagus officinalis L.*


    BIOCELL, Vol.29, No.3, pp. 313-317, 2005, DOI:10.32604/biocell.2005.29.313

    Abstract Callus growth and plant regeneration from long-term callus cultures were studied in two elite clones of Asparagus officinalis cv. Argenteuil, to establish a suitable protocol for a prospective in vitro selection program. Callus initiation and growth was evaluated on MS medium with 3% sucrose, 0.9% agar, 1 mg.l-1 kinetin, and three levels of 2,4-D. The highest callus relative growth was obtained on medium with 1.5 mg.l-1 2,4-D and 1 mg.l-1 kinetin. Shoot primordia (SP) induction from >18-months-old calluses was evaluated on several media; the highest percentage of SP induction (89%) and average number of SP per callus (8.6) were obtained… More >

  • Open Access


    Agrobacterium rhizogenes vs auxinic induction for in vitro rhizogenesis of Prosopis chilensis and Nothofagus alpina


    BIOCELL, Vol.27, No.3, pp. 311-318, 2003, DOI:10.32604/biocell.2003.27.311

    Abstract The induction and improvement of in vitro rhizogenesis of microshoots of Prosopis chilensis (Mol.) Stuntz and Nothofagus alpina (Poep. et Endl. Oerst.) were compared using Agrobacterium rhizogenes (Ar) versus indole-3-butyric acid (IBA) in the culture media. Microshoots of P. chilensis (1-2 cm length), coming from in vitro grown seedlings, were cultivated in a modified Broadleaved Tree Medium (BTMm) containing half salt concentration of macronutrients and 0.05 mg.L-1 benzilaminopurine (BAP). After 30 days, microshoots with 2-4 leaves were selected and cultured in BTMm-agar in presence or abscense of Ar and in combination with IBA. For N. alpina, the apical shoots with… More >

  • Open Access


    Indole-3-butyric acid on rooting and endogenous plant hormones in tetraploid and diploid Robinia pseudoacacia hardwood cuttings

    Wang XL1,2, Z Zhao1, JE Quan1

    Phyton-International Journal of Experimental Botany, Vol.80, pp. 93-100, 2011, DOI:10.32604/phyton.2011.80.093

    Abstract Robinia pseudoacacia (locust hereafter) is an ornamental tree with various uses. Both homologous tetraploid (tetraploid hereafter) and diploid cultivars are commercially available. The tetraploids have advantageous traits, but poor germinability, and cuttings are difficult to root. Since auxin applications can promote rooting, we evaluated the effects of dipping cuttings in various indole-3-butyric acid (IBA) concentrations for four hours on rooting parameters. IBA significantly affected all measured parameters of both tetraploid and diploids cuttings. Tetraploid cuttings produced no roots without IBA. The effects were strongest at 1000 and 800 mg/L IBA for tetraploids and diploids, respectively. Values for tetraploids and diploids… More >

  • Open Access


    Micropropagation of Prosopis chilensis (Mol.) Stuntz from young and mature plants


    BIOCELL, Vol.26, No.1, pp. 25-33, 2002, DOI:10.32604/biocell.2002.26.025

    Abstract Prosopis chilensis (Mol.) Stuntz (Algarrobo de Chile) is an important native tree species that can be grown in arid and semiarid regions for wood and forage production and environmental protection. Developing a simple and reliable in vitro protocol for cloning it would enable to improve it genetically. Explants of P.chilensis were taken from 4 months-old plants grown in the greenhouse or from adult trees grown in a natural environment. Nodal segments 1 – 2 cm long containing an axillary bud were selected from elongating shoots. These cuttings were aseptically cultured on two agar-solid basal media, MS or BTMm, and treated… More >

Displaying 1-10 on page 1 of 7. Per Page