Optimization of Factors Influencing Adventitious Rooting in Hybrid Larch
- Kuipeng Li1,2, Hua Han1, Yunhui Xie1, Xiaomei Sun1,*
1 State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of State Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, China
2 Guangxi Key Laboratory of Superior Timber Trees Resource Cultivation & Key Laboratory of Central South Fast-Growing Timber Cultivation of Forestry Ministry of China, Guangxi Forestry Research Institute, Nanning, 530002, China
* Corresponding Author: Xiaomei Sun. Email:
(This article belongs to this Special Issue: Tree Somatic Embryogenesis and Application)
2 Guangxi Key Laboratory of Superior Timber Trees Resource Cultivation & Key Laboratory of Central South Fast-Growing Timber Cultivation of Forestry Ministry of China, Guangxi Forestry Research Institute, Nanning, 530002, China
* Corresponding Author: Xiaomei Sun. Email:
(This article belongs to this Special Issue: Tree Somatic Embryogenesis and Application)
Received 26 August 2020; Accepted 21 September 2020; Issue published 07 February 2021
Abstract
Optimization of in vitro adventitious root induction contributes to the development of a large-scale production
system of hybrid clone seedlings of larch (Larix spp.). We used orthogonal testing to investigate the factors that
affect the rooting of hybrid larch shoots–activated carbon, vitamin B1, sucrose, glycine, glutamic acid (Glu),
cysteine, pH, and induction time in the dark and indole-3-butyric acid (IBA). Variance analysis showed that
the effects of sucrose concentration on rooting rate, survival rate, and average root number, pH on the average
number of roots; and Glu on survival rate were all significant (p < 0.05) when compared to the mock treatments.
Among all of the 45 treatments, the highest rooting rate was 90%, the highest survival rate was 100%, and the
highest average root number was 11.8. The theoretically optimal procedure of in vitro root induction of hybrid
larch shoots is shoot induction on L9 modified medium (pH 6.7) containing 2 mg.L–1 naphthalene acetic acid,
0.25 mg.L–1 IBA, 1/3 macroelements, 7.5 g.L–1 sucrose, and 5 mg.L–1 Glu for 8 days, and then root induction
on L9 modified medium without auxin.
Keywords
Hybrid larch; adventitious root; orthogonal test; variance analysis
Cite This Article
Li, K., Han, H., Xie, Y., Sun, X. (2021). Optimization of Factors Influencing Adventitious Rooting in Hybrid Larch. Phyton-International Journal of Experimental Botany, 90(2), 583–593.
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