Special Issue "Tree Somatic Embryogenesis and Application"

Submission Deadline: 30 June 2021 (closed)
Guest Editors
Jinhui Chen, Professor, Nanjing Forestry University, Nanjing 210037, China
Xiangyang Hu, Professor, Shanghai University, Shanghai, 200444, China


Somatic embryogenesis (SEs) technique is one of important tool to study plant embryo development and regulation, as well as the industrial breeding of rare and endogenous tree species, thus shows essential for fundamental research or production application. it allows the long-term ex situ conservation of genetic resources by cryopreservation techniques. For some woody species and in particular for some coniferous trees, somatic embryogenesis induction has become a routine procedure. For the majority, the application of this technology presents yet many limitations especially due to the genotype, the induction conditions, the number of embryos produced, maturation, and conversion, among other factors that compromise the systematic use of somatic embryogenesis for commercial purposes especially of woody species and forest trees in particular. In recent years there has been major improvement in tree SEs, this special issue will describe progress in SEs of tree SEs and its application in tree breeding and application.

This special issue is proposed to explore the most recent development and innovation occurs during recent years in the field of tree somatic embryogenesis and application. The manuscripts address the following topics:
1) the novel fundamental of tree somatic embryogenesis

2) the key regulatory factor to determine tree somatic embryogenesis

3) the omics study of tree somatic embryogenesis

3) the tree tissue culture and regeneration

4) the new or high-efficiency technique for transgenic tree

5) the genome editing for tree

6) the industrial application of tree somatic embryogenesis

Tree, Somatic Embryogenesis, Industrial Application

Published Papers
  • Vital Parameters Assessments of Starvation Tolerance of in vitro Populus alba Culture
  • Abstract Populus alba is a large woody deciduous plant. The plant has been introduced to shooting, then multiplication of rooting on Murashige and Skoog (MS) medium. This work was designed to estimate the effect of two factors (low levels of 1-Naphthaleneacetic acid NAA and sucrose) on P. alba response resulting in 6 treatments compared to the control, with twelve measured responses. There was a significant difference in some measurements in morphology, like plantlets fresh-weight, shoot-, root-length, and leaf number. In the physiological measurements, there were significant differences in all the measured parameters. The low concentrations of sucrose and media composition/power (MS… More
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  • Suspension Culture and Somatic Embryogenesis of Korean Pine
  • Abstract Korean pine is an important afforestation tree species in Northeast China, which has a high ecological and economic value. Although regeneration of somatic embryogenesis using immature zygotic embryos of Korean pine as explants has been successful, it cannot be applied to automation and large-scale production. Therefore, we urgently need a method that can increase the output of somatic embryos (SEs) to meet the needs of large-scale production. We used Korean pine 1–1 and 1–100 cell lines as research materials to evaluate the effects of inoculum-density, culture time, orbiting speed, vessel volume, plant growth regulator (PGR) concentration, and carbon source on… More
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  • Encapsulation of Immature Somatic Embryos of Coffea arabica L. for in Vitro Preservation
  • Abstract The present study aimed to develop a protocol for somatic embryogenesis and encapsulation of coffee embryos (Coffea arabica L.), for the conservation of genotypes with characteristics of commercial interest. Somatic embryos were induced from leaf explants in Murashige and Skoog medium (MS) supplemented with 1 mg · L−1 of 2,4-dichlorophenoxiacetic acid (2,4-D) combined with 2 mg · L−1 of benzyladenine (BA). Somatic embryos (SE) at the globular stage were encapsulated in a sodium alginate matrix; two treatments were tested: MS + 5 mg · L−1 BA + 1 mg · L−1 NAA + 3% (w/v) alginate, and MS + 7 mg · L−1 BA + 5.7 mg · L−1 indoleacetic acid (IAA) + 3% (w/v) alginate. Alginate was… More
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  • The Regulatory Roles of microRNAs and Associated Target Genes during Early Somatic Embryogenesis in Liriodendron Sino-Americanum
  • Abstract Somatic cells respond to considerable stress, and go through a series of phytohormone pathways, then forming an embryo. The developmental process is recorded as somatic embryogenesis (SE). One of the key components regulating SE are the microRNAs (miRNAs). Despite previous studies, it is still not clear exactly how miRNAs exert their function of regulating targets during conditionally activated early SE. Here, we use Liriodendron sino-americanum as a model system and perform a combined analysis of microfluidic chips and degradome sequencing to study this process. We identified a total of 386 conserved miRNAs and 153 novel miRNAs during early SE. According… More
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  • Efficient Evergreen Plant Regeneration of Cinnamomum japonicum Sieb. through in vitro Organogenesis
  • Abstract Cinnamomum japonicum Sieb. is an excellent roadside tree and medicinal tree species with considerable ornamental and economic value. In this study, we successfully developed a large-scale micropropagation protocol for C. japonicum for the first time. Sterilized shoots were excised and used as explants for shoot induction on several basal media, supplemented with different concentrations of plant growth regulators (PGRs), such as Thidiazuron (TDZ), N6 -Benzyladenine (6-benzylaminopurine) (BA), α-naphthaleneacetic acid (NAA) and Gibberellic acid (GA3). After comparison, the most efficient medium for shoot regeneration was 1/2 Murashige and Skoog (MS) medium containing 0.5 mg L–1 BA, 0.05 mg L–1 NAA and… More
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  • Optimization of Factors Influencing Adventitious Rooting in Hybrid Larch
  • Abstract Optimization of in vitro adventitious root induction contributes to the development of a large-scale production system of hybrid clone seedlings of larch (Larix spp.). We used orthogonal testing to investigate the factors that affect the rooting of hybrid larch shoots–activated carbon, vitamin B1, sucrose, glycine, glutamic acid (Glu), cysteine, pH, and induction time in the dark and indole-3-butyric acid (IBA). Variance analysis showed that the effects of sucrose concentration on rooting rate, survival rate, and average root number, pH on the average number of roots; and Glu on survival rate were all significant (p < 0.05) when compared to the… More
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  • An Efficient Plant Regeneration System of Hydrangea bretschneideri Dipp via Stem Segments as Explants
  • Abstract Hydrangea bretschneideri Dipp is a highly popular ornamental plant for garden decoration. Genetic engineering technology has been successfully used in many plant species, but it is limited in Hydrangea. Here we established an efficient regeneration system by using stem segments as explants for the first time. In our study, the plant growth regulators (PGRs) were evaluated at the different regeneration processes, including axillary shoots regeneration and root induction. We found that the optimal concentration for axillary buds’ induction was 2.0 mgL−1 6-BA and 0.5 mgL−1 1 IAA, its highest induction rate was 70%. Moreover, the highest axillary shoots proliferation coefficient… More
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