Gang Bao1, Andrew Tsourkas2, Philip J. Santangelo2
Molecular & Cellular Biomechanics, Vol.1, No.1, pp. 23-36, 2004, DOI:10.3970/mcb.2004.001.023
Abstract The ability to detect, localize, quantify and monitor the expression of specific genes in living cells in real-time will offer unprecedented opportunities for advancement in molecular biology, disease pathophysiology, drug discovery, and medical diagnostics. However, current methods for quantifying gene expression employ either selective amplification (as in PCR) or saturation binding followed by removal of the excess probes (as in microarrays and in situ hybridization) to achieve specificity. Neither approach is applicable when detecting gene transcripts within living cells. Here we review the recent development in engineering nanostructured molecular probes for gene detection in vivo, describe More >
Login
Register
