Open Access

ARTICLE

Granulocyte colony-stimulating factor decreases tumor necrosis factor production in whole blood: role of interleukin-10 and prostaglandin E₂

Davide Agnello¹, Patrizia Mascagni³, Riccardo Bertini³, Pia Villa^1,2, Giorgio Senaldi⁴, Pietro Ghezzi¹

1 « Mario Negri » Institute for Pharmacological Research
2 CNR, Institute of Neuroscience, Cellular and Molecular Pharmacology Section, Milan, Italy
3 Dompé Research Center, L’Aquila, Italy
4 Amgen Inc., Thousand Oaks, California, USA

* Corresponding Author: P. Ghezzi,

European Cytokine Network 2004, 15(4), 323-326.

Abstract

Previous reports have indicated that the administration of granulocyte colony-stimulating factor (G-CSF) decreases ex vivo tumor necrosis factor (TNF) production in humans. In this study, we report that daily pretreatment of mice with G-CSF for three days decreases ex vivo lipopolysaccharide (LPS)-induced TNF production in whole blood. Conversely, production of interleukin-10 (IL-10) and prostaglandin E₂ (PGE₂) is increased. The inhibitory effect of G-CSF pretreatment on TNF production is partially reversed by addition of an anti-IL-10 antibody, and completely reversed by combined addition of anti-IL-10 antibody and the cyclooxyge-nase (COX) inhibitor, ketoprofen. These results suggest that G-CSF decreases TNF production in this experimental model by increasing production of IL-10 and PGE₂, which are both known inhibitors of TNF production.

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Keywords

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