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Cytokine production by peripheral lymphocytes in melanoma
1 Department of Dermatology, Instituto Valenciano de Oncología, Valencia
2 Department of Biochemistry and Molecular Biology, School of Medicine, University of Valencia
3 Department of Epidemiology, Hospital Universitario La Fe, Valencia
* Corresponding Author: Rafael Botella-Estrada,
European Cytokine Network 2005, 16(1), 47-55.
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Background. The differentiation of T cells towards a T helper 1 (Th1) or Th2 phenotype based on their profile of cytokine production, is of great relevance in the regulation of immune responses. We have determined by flow cytometry, the expression of selected Th1 and Th2 cytokines by activated T cells in whole blood samples (WB) from normal donors and from patients with different clinical stages of melanoma in different clinical stages. Methods. WB samples from 6 normal donors and 19 patients with melanoma were activated over 4 hours with PMA + ionomycin in presence or absence of a protein secretion inhibitor. Following surface staining (CD3-Cy5+CD8-FITC), fixation and permeabilization, cells were stained with PE-labelled antibodies against Th1 cytokines (IL-2, IFN-γ, TNF-α) and Th2 cytokines (IL-4, IL-10). Results. The most relevant results were related to IFN-γ and IL-10 production. The percentage of IFN-γ producer cells was significantly lower in melanoma patients, independent of the stage, than in controls. IL-10 production was significantly increased in melanoma patients with respect to normal donors. Conclusions. Our data support the notion that the pattern of cytokines produced by lymphocytes from melanoma patients may help to explain the impairment in their T cell immune response. More extensive studies regarding the pattern of cytokines, not only in peripheral blood, but also in tumour tissue and sentinel lymph nodes, are needed to confirm these data.Keywords
melanoma, cytokines, Th1, Th2
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APA Style
Botella-Estrada, R., Escudero, M., O’Connor, J.E., Nagore, E., Fenollosa, B. et al. (2005). Cytokine production by peripheral lymphocytes in melanoma. European Cytokine Network, 16(1), 47–55.
Vancouver Style
Botella-Estrada R, Escudero M, O’Connor JE, Nagore E, Fenollosa B, Sanmartín O, et al. Cytokine production by peripheral lymphocytes in melanoma. Eur Cytokine Network. 2005;16(1):47–55.
IEEE Style
R. Botella-Estrada et al., “Cytokine production by peripheral lymphocytes in melanoma,” Eur. Cytokine Network, vol. 16, no. 1, pp. 47–55, 2005.
Copyright © 2005 The Author(s). Published by Tech Science Press.This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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